Distribution of calcium binding protein mRNAs in rat cerebellar cortex

Kadowaki, Koji; McGowan, Eileen; Mock, Graham A.; Chandler, Stephen F.; Emson, Piers C.

doi:10.1016/0304-3940(93)90082-v

Cited by 31 publications

(11 citation statements)

References 17 publications

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“…Purkinje cells show a strong PV-and CB-immunoreactivity, as previously reported (Rogers et al, 1990;Heizmann and Braun, 1990;review: Andressen et al, 1993). Calretinin is strongly expressed by cerebellar granule cells (Rogers, 1989;Heizmann and Hunziker, 1991;Kadowaki et al, 1993). The same cell expression patterns were found in the cerebellum of Meriones and Monodelphis, serving as positive control (not shown).…”

Section: Positive Control For Cabp-immunolabelingsupporting

confidence: 57%

Characterization of cochlear nucleus principal cells of Meriones unguiculatus and Monodelphis domestica by use of calcium-binding protein immunolabeling

Bazwinsky

Härtig²,

Rübsamen³

2008

Journal of Chemical Neuroanatomy

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Section: Positive Control For Cabp-immunolabelingsupporting

confidence: 57%

Characterization of cochlear nucleus principal cells of Meriones unguiculatus and Monodelphis domestica by use of calcium-binding protein immunolabeling

Bazwinsky

Härtig²,

Rübsamen³

2008

Journal of Chemical Neuroanatomy

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“…Previous reports using ISHH demonstrated that cerebellar calretinin mRNA was localized in granule cells (Kadowaki et al, 1993). Studies using immunohistochemistry also located cerebellar calretinin immunoreactivity mainly in granule cells and their parallel fibers in the molecular layer (Rogers, 1989;Arai et al, 1991;Résibois and Rogers, 1992).…”

Section: Resultsmentioning

confidence: 93%

Decreased calretinin expression in cerebellar granule cells in the leaner mouse

2002

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We investigated calretinin expression in cerebellar granule cells of 30-day-old leaner mice to understand possible changes in calcium homeostasis due to the calcium channel mutation that these mice carry. Quantitative in situ hybridization histochemistry showed decreased calretinin mRNA expression in the leaner cerebellum. Immunohistochemical staining also revealed decreased calretinin immunoreactivity in the leaner cerebellum. To exclude the effect of granule cell loss that occurs in the leaner mouse when comparing cerebellar calretinin expression, the number of granule cells per unit area in the cerebellum was compared to the wild-type cerebellum. Granule cell counts per unit area of cerebellum revealed similar numbers of granule cells present in wild-type and leaner mice. Laser capture microdissection (LCM) was employed to obtain an equal number of granule cells from wild-type and leaner mice. Western blot analysis with LCM-procured cerebellar granule cells showed decreased calretinin expression in leaner granule cells. These results indicate that there is an absolute decrease in calretinin expression in leaner granule cells even when granule cell loss is taken into account. Decreased calretinin expression in leaner granule cells may contribute to altered calcium buffering capacity. This alteration could be an adaptive change due to the calcium channel dysfunction, and may result in abnormal neuronal excitability and gene expression.

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“…As the neuron type with the largest somata in the cerebellum, PCs can be identified by soma size and several molecular markers such as calbindin (Calb1) ; [25] and the T-type calcium channel (Cacna1g) [26,27]. Moreover, PCs can be identified by spatial localization as these cells are found forming a distinct lamina (Purkinje cell layer; PCL) that is one PC thick, separating the molecular layer from the granule cell layer.…”

Section: Resultsmentioning

confidence: 99%

Neuronal Migration Defect of the Developing Cerebellar Vermis in Substrains of C57BL/6 Mice: Cytoarchitecture and Prevalence of Molecular Layer Heterotopia

Mangaru

Salem²,

Sherman³

et al. 2013

Dev Neurosci

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Abnormal development of the cerebellum is often associated with disorders of movement, postural control, and motor learning. Rodent models are widely used to study normal and abnormal cerebellar development and have revealed the roles of many important genetic and environmental factors. In the present report we describe the prevalence and cytoarchitecture of molecular-layer heterotopia, a malformation of neuronal migration, in the cerebellar vermis of C57BL/6 mice and closely-related strains. In particular, we found a diverse number of cell-types affected by these malformations including Purkinje cells, granule cells, inhibitory interneurons (GABAergic and glycinergic), and glia. Heterotopia were not observed in a sample of wild-derived mice, outbred mice, or inbred mice not closely related to C57BL/6 mice. These data are relevant to the use of C57BL/6 mice as models in the study of brain and behavior relationships and provide greater understanding of human cerebellar dysplasia.

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Distribution of calcium binding protein mRNAs in rat cerebellar cortex

Cited by 31 publications

References 17 publications

Characterization of cochlear nucleus principal cells of Meriones unguiculatus and Monodelphis domestica by use of calcium-binding protein immunolabeling

Characterization of cochlear nucleus principal cells of Meriones unguiculatus and Monodelphis domestica by use of calcium-binding protein immunolabeling

Decreased calretinin expression in cerebellar granule cells in the leaner mouse

Neuronal Migration Defect of the Developing Cerebellar Vermis in Substrains of C57BL/6 Mice: Cytoarchitecture and Prevalence of Molecular Layer Heterotopia

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