Distribution of calcium‐ATPase in developing teeth of embryonic American alligators (Alligator mississippiensis)

Abstract

Light microscopic and ultrastructural observations were carried out to evaluate the cell morphology and histochemistry (calcium-ATPase activity) of developing teeth in embryonic American alligators (Alligator mississippiensis). Ca-ATPase activity was observed in the distal and lateral cell membranes, rough endoplasmic reticulum (rER), mitochondria, vacuoles, and other organelles of the ameloblast, but only in the distal cell membrane and process of the odontoblast. Enzyme activity in the ameloblasts increased gradually during development. These sites of enzyme activity are related to mineralization of the enamel layer, similar to that in mammalian tooth development. Alligator teeth are heavily mineralized like mammalian teeth; however, alligator ameloblasts have undeveloped distal processes during mineralization in contrast to mammalian ameloblasts in which Tomes' processes are found near the distal portion of ameloblasts at maturation stage. The localization of intense enzyme activity in the distal and lateral ameloblast cell membrane suggests that these regions are the site of accumulation of calcium as enamel differentiates in the developing tooth. © 1993 Wiley-Liss, Inc.

Altered localization of Cav1.2 (L‐type) calcium channels in nerve fibers, Schwann cells, odontoblasts, and fibroblasts of tooth pulp after tooth injury

“…The odontoblasts are involved actively in the transport and accumulation of Ca 2+ ions to the mineralization region during dentin formation. It is well documented that Na + /Ca 2+ antiports and a Ca 2+ ‐ATPase are two of the Ca 2+ extrusion mechanisms present in the odontoblast membrane (Lundgren and Linde, 1988; Borke et al, 1993; Sato et al, 1993). Only recently has work been done to investigate the mechanisms of Ca 2+ entry into these cells.…”

Evolution of patterns and processes in teeth and tooth‐related tissues in non‐mammalian vertebrates

“…Enamel mineralization follows a pattern similar to that in mammals, at least in developing frog teeth (289). SATO and coworkers (281) localized intense Ca-ATPase activity in the distal and lateral cell membranes of alligator ameloblasts sugge ting that, during tooth maturation, these regions are the sit es of accumulation of calcium prior to tran ·port to the enamel layer. Trace element in the enamel (e.g.…”

Plasma Membrane Ca2+–ATPase in Rat and Human Odontoblasts Mediates Dentin Mineralization

“…Although the ablation of t in early embryonic lethality (highlighting the essential role of PM organogenesis, and housekeeping function [12]), the deletion of PM associated with reduced bone mineralization [34]. In addition, o blasts, which form hard tissues and play roles in bone mineraliza respectively, also express PMCA [16,17,35,36]. In ameloblasts, PM gradually during developmental processes, suggesting that PMCA ciated with amelogenesis and the maturation of ameloblasts [16,3 differentiation, immunohistochemical analysis using monoclon erythrocyte PMCA revealed a lack of immunoreactivity in the prethe surface of the dental papilla, while gradient increases in the im were observed in the period when the odontoblast became fully di allel to the progression of mineralization of dentin [17].…”

“…Although the ablation of the PMCA1 gene results in early embryonic lethality (highlighting the essential role of PMCA1 in development, organogenesis, and housekeeping function [12]), the deletion of PMCA1 in the intestine is associated with reduced bone mineralization [34]. In addition, osteoblasts and ameloblasts, which form hard tissues and play roles in bone mineralization and amelogenesis, respectively, also express PMCA [16,17,35,36]. In ameloblasts, PMCA activity increases gradually during developmental processes, suggesting that PMCA in ameloblasts is associated with amelogenesis and the maturation of ameloblasts [16,36].…”

“…Odontoblasts have been shown to express PMCA in the distal cell membrane and its processes [16,17]. Lundgren andLinde (1988 and previously demonstrated ATPdependent Ca 2+ extrusion, most likely by Ca 2+ -ATPase, across the plasma membrane in odontoblasts [18,19].…”