Distribution of cadmium, zinc and copper in the musselMytilus edulis. Existence of cadmium-binding proteins similar to metallothioneins

Noël-Lambot, F.

doi:10.1007/bf01940817

Cited by 148 publications

(31 citation statements)

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“…Metallothioneins have been found in some invertebrates too (Noel-Lambot, 1976, 1979Howard & Nickless, 1977;Noel-Lambot et al, 1978). However, their significance for Cd detoxification in these animals is unknown.…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, metallothioneins are responsible for primary accumulation of the metal in kidney and liver of the test animals (Tanaka et al, 1975;Overnell et al, 1977). Olafson & Thompson (1974) assumed a global presence of metallothioneins and in recent years their occurrence in some marine invertebrates has been established too (Noel-Lambot, 1976;Howard & Nickless, 1977). Nevertheless, little is known about their function in invertebrate metabolism.…”

Section: Introductionmentioning

confidence: 99%

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Accumulation, loss and molecular distribution of cadmium inMytilus edulis

Scholz

1980

Helgolander Meeresunters

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In Mytilus edulis, accumulation and loss of Cd were analyzed under experimental conditions. Cd uptake by the whole soft body is linear, increasing significantly with increasing Cd concentrations in the uptake medium. Until 100 #g Cd 1-1, neither limitation of uptake nor any saturation process can be observed. Loss of Cd, measured after transfer of experimentally contaminated mussels to natural sea water, is exponential; biological half lives vary between 14 and 29 days. Gills are the primary sites of Cd uptake from the water, whereas in mid-gut gland, kidney, and mantle the uptake is retarded during the first few days. The mid-gut gland not only bears the main body load of Cd, but also shows the highest Cd concentrations. Gel chromatographic studies of midgut gland proteins reveal that Cd is eluated over the whole molecular weight range. Three metallothionein-like proteins with molecular weights of 6,600, 13,200, and 21,000 Dalton could be established. However, they cannot be taken as effective detoxification proteins, because more than 50 % of the accumulated metal is bound to high molecular weight proteins.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Accumulation, loss and molecular distribution of cadmium inMytilus edulis

Scholz

1980

Helgolander Meeresunters

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“…Different metal cation detoxification systems have therefore been developed in organisms (Viarengo and Nott, 1993). A common detoxification system is based upon soluble ligands, i.e., metal-binding proteins such as metallothioneins or phosphoproteins (Margoshes and Vallee, 1957;Noel-Lambot, 1976;Marsh and Sass, 1985). These proteins may become part of the EPF.…”

Section: Chemical Composition Of the Insoluble Organic Matrixmentioning

confidence: 99%

Effect of organic matrices on the determination of the trace element chemistry (Mg, Sr, Mg/Ca, Sr/Ca) of aragonitic bivalve shells (<i>Arctica islandica</i>)—Comparison of ICP-OES and LA-ICP-MS data

Schöne¹,

Zhang²,

Jacob³

et al. 2010

Geochem. J.

106

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The element chemistry of biogenic carbonates can provide important data on past environments. However, the Sr/Ca and Mg/Ca ratios as well as the Mg and Sr concentrations of biological carbonates, especially aragonitic bivalves often depart from apparent thermodynamic equilibrium. When measured in situ by means of LA-ICP-MS, the Mg concentration is often substantially enriched (two-to threefold) near the organic-rich, annual growth lines. To test the hypothesis that some organic components exert a major influence on the skeletal metal content, the element chemistry of different shell components (insoluble organic matrix, IOM; dissolved CaCO 3 and soluble organics, SOM) of Arctica islandica was measured by means of ICP-OES and LA-ICP-MS. The ICP-OES data indicate that the IOM is strongly enriched in Mg (130 ppm) and depleted in Sr and Ca (10 ppm and 0.22 wt%, respectively) when compared to the whole biomineral (Mg: 68 to 99 ppm, Sr: 860 to 1,060 ppm, Ca: ~35.72 wt%). Although the average relative abundance of the IOM barely exceeds 0.46 wt%, its chemical composition in combination with its heterogeneous distribution across the shell can significantly increase estimates of the Mg concentration if measured in situ by LA-ICP-MS. Depending on the distribution of the IOM, the Ca concentration may be significantly lower locally than the average Ca concentration of the whole shell (35.72 wt%). If this remains undetected, the Mg concentration of shell portions with higher than average IOM content is overestimated by LA-ICP-MS and, conversely, the Mg concentration is underestimated in shell portions with lower than average IOM content. Removal of the IOM prior to the chemical analysis by LA-ICP-MS or mathematical correction for the IOMderived magnesium concentrations is therefore strongly advised. The different chemistry of the IOM may also exert a major control on the trace element to calcium ratios. Shell portions enriched in IOM will show up to 200 times higher Mg/ Ca and up to two times higher Sr/Ca ratios than the average shell of A. islandica. Without removal of the IOM prior to the analysis, Mg/Ca and Sr/Ca ratios of shell portions with higher IOM content cannot be used as paleothermometers. Because it is currently impossible to remove the IOM prior to chemical analyses by LA-ICP-MS, we recommend the use of wet chemical techniques (= possibility to separate and measure individual shell components) such as ICP-OES at the expense of lower sampling resolution. The results of this study will significantly improve our understanding of shellbased climate and environmental proxies.

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“…Metallothioneins are induced by heavy metals in the livers of many species, including rat [4,5], rabbit [6], chicken [7] and fish [8]. Metallothioneins are also induced by metals in crustacea [9], mollusks [9,10], yeast [I 11, and Neurospora [12]. Metallothioneins can also be induced by heavy metals in a variety of cultured cells, including primary rat hepatocytes [I3 -151, pig kidney cells [16], human skin epithelial cells [17], Chinese hamster ovary cells [18], clonal rat hepatoma cells [19], and HeLa cells [20].…”

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confidence: 99%

Induction of Metallothionein mRNA in HeLa Cells by Dexamethasone and by Heavy Metals

1981

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Synthesis of metallothionein, a cysteine-rich heavy metal binding protein, is induced in cultured HeLa cells both by the heavy metals CdZ+, Zn2+ and Cu2+, and by the glucocorticoid hormone dexamethasone. The accumulation of [35S]cysteine-labeled metallothionein and the amount of translatable metallothionein mRNA show identical concentration dependences in response to dexamethasone treatment and in response to zinc exposure. Induction of translatable metallothionein mRNA is rapid in response to both the metal and glucocorticoid inducers. Increased synthesis and accumulation of metallothionein in response to either metal or glucocorticoid exposure is regulated by the level of translatable metallothionein mRNA in HeLa cells.Metallothioneins are low-molecular-weight proteins which bind the heavy metals ZnZf, CdZ+, Hg", Ag2+ and Cu2+. [20].Hepatic metallothioneins are also induced by various types of stress in rats [21]. The stress-related induction of metallothioneins in vivo suggested that this protein might be regulated by hormonal mechanisms. We have recently demonstrated the induction of metallothionein by the synthetic glucocorticoid dexamethasone, both in HeLa cells [22] and in primary hepatocytes [I 51. The induction of metallothionein by glucocorticoids in HeLa cells is mediated through the cbassic glucocorticoid receptor [23]. The metallothioneins induced in HeLa cells by Zn2+ and glucocorticoids are interia [24], although Zn2+ and glucocorticoid hormones appear to induce metallothioneins by mechanisms independent of one another in these cells [25].In rat liver, metallothionein induction by heavy metals is mediated through an increase in translatable metallothionein mRNA [26,27]. Experiments using actinomycin D and cordycepin suggest that metal regulation of metallothionein synthesis in rat liver is probably at the level of available MRNA [28]. Enger et al. [29] MATERIALS AND METHODS ChemicalsTranslation grade [35S]cysteine (> 600 Ci/mmol) was obtained from New England Nuclear; [3H]cysteine (0.5-2 Ci/ mmol) and [3H]leucine (60 Ci/mmol) were from Amersham. Phenol was distilled under N2 and stored at -20°C. lodoacetic acid was recrystallized from light petroleum (b.p. 30-60 "C). Dexamethasone and dithiothreitol were from Sigma.All other chemicals were reagent grade. Guanidine . HCI (ultrapure) was from Schwartz-Mann. Nonidet P40 was from Bethesda Research Labs.Cell Culture in a serum-free hormone-supplemented medium.HeLa cells were grown as described previously [20.22,30] RNA Extraction HeLa cells from three confluent 100-mm plates were scraped into 1.4 ml of ice-cold phosphate-buffered saline and collected by centrifugation in a Beckman microfuge B for 45 s. The pellet was resuspended in 0.35 ml Tris/saline buffer (0.01 M Tris/HCl, pH 7.0; 0.15 M NaCI, 2 mM MgC12) containing 0.5 "/, Nonidet P40 and lysed for 5 min on ice. Nuclei were pelleted by centrifugation for 2 min in a Beckman microfuge and RNA was extracted from the supernatant by one of two different extraction procedures. In the case of the p...

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Distribution of cadmium, zinc and copper in the musselMytilus edulis. Existence of cadmium-binding proteins similar to metallothioneins

Cited by 148 publications

References 10 publications

Accumulation, loss and molecular distribution of cadmium inMytilus edulis

Accumulation, loss and molecular distribution of cadmium inMytilus edulis

Effect of organic matrices on the determination of the trace element chemistry (Mg, Sr, Mg/Ca, Sr/Ca) of aragonitic bivalve shells (<i>Arctica islandica</i>)—Comparison of ICP-OES and LA-ICP-MS data

Induction of Metallothionein mRNA in HeLa Cells by Dexamethasone and by Heavy Metals

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Distribution of cadmium, zinc and copper in the musselMytilus edulis. Existence of cadmium-binding proteins similar to metallothioneins

Cited by 148 publications

References 10 publications

Accumulation, loss and molecular distribution of cadmium inMytilus edulis

Accumulation, loss and molecular distribution of cadmium inMytilus edulis

Effect of organic matrices on the determination of the trace element chemistry (Mg, Sr, Mg/Ca, Sr/Ca) of aragonitic bivalve shells (<i>Arctica islandica</i>)&mdash;Comparison of ICP-OES and LA-ICP-MS data

Induction of Metallothionein mRNA in HeLa Cells by Dexamethasone and by Heavy Metals

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Effect of organic matrices on the determination of the trace element chemistry (Mg, Sr, Mg/Ca, Sr/Ca) of aragonitic bivalve shells (<i>Arctica islandica</i>)—Comparison of ICP-OES and LA-ICP-MS data