Distribution of c-Fos in guinea-pig brain following morphine withdrawal

Chahl, Loris A.; Leah, J.D.; Herdegen, Thomas; Trueman, L.; Lynch-Frame, Ann

doi:10.1016/0006-8993(96)00041-8

Cited by 17 publications

(9 citation statements)

References 21 publications

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“…The Fos‐related antigen expression in control animals in the present experiments was higher than seen in previous experiments in which repeated subcutaneous injections of drugs were used (Chahl et al ., 1996). This might have been due to the stressful effect of repeated i.m.…”

Section: Discussioncontrasting

confidence: 80%

The anabolic androgenic steroid, nandrolone decanoate, increases the density of Fos‐like immunoreactive neurons in limbic regions of guinea‐pig brain

Johansson-Steensland

Nyberg

Chahl

2002

Eur J of Neuroscience

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The increased abuse of anabolic androgenic steroids is a major concern because of physiological and psychological side-effects. In some individuals they induce dramatic behavioural changes such as increased aggression, anxiety and depression. The mechanisms behind these behavioural changes are still poorly understood. In order to obtain information on the brain regions affected by anabolic androgenic steroids, the distribution of neurons containing c-Fos, the protein product of the immediate early gene c-fos, and Fos-related antigens was studied following chronic treatment of guinea-pigs with a high dose of nandrolone decanoate (15 mg/kg i.m. daily for 14 days). The behaviour of the guinea-pigs was monitored for 1 h each day. Animals treated with nandrolone exhibited a significantly greater incidence of biting behaviour during the 14 day treatment period than vehicle-treated animals. A significantly greater density of c-Fos and Fos-related antigen-positive neurons was found in the central nucleus of the amygdala, and of Fos-related antigen-positive neurons in the frontal cortex, the shell of the nucleus accumbens and the supraoptic nucleus in nandrolone-treated animals than in vehicle controls. Therefore, nandrolone induced Fos in brain regions involved in stress, behavioural responses and reward. The increased Fos expression in these limbic brain regions is of particular interest in relation to the behavioural changes reported in humans who abuse anabolic androgenic steroids and the abuse potential of these drugs.

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Section: Discussioncontrasting

confidence: 80%

The anabolic androgenic steroid, nandrolone decanoate, increases the density of Fos‐like immunoreactive neurons in limbic regions of guinea‐pig brain

Johansson-Steensland

Nyberg

Chahl

2002

Eur J of Neuroscience

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“…For example, this activating effect would possibly explain the c‐fos induction observed in the LC. Indeed, an increased expression of c‐fos mRNA during morphine withdrawal has been described in this structure which showed an intense expression of µ opiate receptors (Hayward et al ., 1990; Couceyro & Douglass, 1995; Chahl et al ., 1996). Alternatively, it is possible that other neurotransmitters, such as glutamate, mediate a part of the c‐fos mRNA induction in the LC, as previously shown in electrophysiological and behavioural studies (Rasmussen, 1995; Rasmussen et al ., 1996; Vandergriff & Rasmussen, 1999).…”

Section: Discussionmentioning

confidence: 99%

Mapping of c‐fos gene expression in the brain during morphine dependence and precipitated withdrawal, and phenotypic identification of the striatal neurons involved

Georges

Stinus

Moine

2000

Eur J of Neuroscience

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The c-fos gene is expressed in the central nervous system in response to various neuronal stimuli. Using in situ hybridization, we examined the effects of chronic morphine treatment and withdrawal on c-fos mRNA in the rat brain, and particularly within identified striatal neurons. Morphine dependence was induced by subcutaneous implantation of two pellets of morphine for 6 days and withdrawal was precipitated by administration of naltrexone. Placebo animals and morphine-dependent rats showed a very weak c-fos mRNA expression in all the structures studied. Our study emphasized the spatial variations in c-fos mRNA expression, and also revealed a peak expression of c-fos mRNA at 1 h after naltrexone-precipitated withdrawal in the projection areas of dopaminergic neurons, noradrenergic neurons and in several regions expressing opiate receptors. Interestingly, morphine withdrawal induces c-fos mRNA expression in the two efferent populations of the striatum (i.e. striatonigral and striatopallidal neurons) both in the caudate putamen and nucleus accumbens. Moreover, the proportions of activated neurons during morphine withdrawal are different in the caudate putamen (mostly in striatopallidal neurons) and in the shell and core parts of the nucleus accumbens (mostly in striatonigral neurons). The activation of striatopallidal neurons suggests a predominant dopaminergic regulation on c-fos gene expression in the striatum during withdrawal. On the contrary, c-fos induction in striatonigral neurons during withdrawal seems to involve a more complex regulation like opioid-dopamine interactions via the mu opioid receptor and the D1 dopamine receptor coexpressed on this neuronal population or the implication of other neurotransmitter systems.

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“…Investigation of the effects of stress‐related stimuli on the expression of the protein products of immediate‐early genes such as c‐fos is an effective means of evaluating the effects of a temporally defined stimulus on large populations of cells, with resolution of functional responses at the single‐cell level. Paradigms associated with increased anxiety or conditioned fear,30‐41 opiate withdrawal,42,43 and intracerebroventricular infusion of CRF or similar anxiogenic neuropeptides44,45 are effective at increasing immediate‐early gene expression within caudal regions of the DR. When these data are taken together with those indicating increased serotonergic neurotransmission in the DR in response to stressful stimuli,46,47 especially when the stressor is intense, uncontrollable, or unpredictable,48‐51 they suggest a mechanism whereby anxiogenic or fear‐inducing stimuli increase serotonergic neurotransmission via actions on subsets of serotonergic neurons within the caudal regions of the DR that give rise to a mesolimbocortical serotonergic innervation of the forebrain.…”

Section: Immediate‐early Gene Expression Studiesmentioning

confidence: 99%

Anatomic and Functional Topography of the Dorsal Raphe Nucleus

Abrams

Johnson

Hollis

et al. 2004

Annals of the New York Academy of Sciences

261

192

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A BSTRACT : Serotonergic systems play an important and generalized role in regulation of sleep-wake states and behavioral arousal. Recent in vivo electrophysiologic recording studies in animals suggest that several different subtypes of serotonergic neurons with unique behavioral correlates exist within the brainstem raphe nuclei, raising the possibility that topographically organized subpopulations of serotonergic neurons may have unique behavioral or physiologic correlates and unique functional properties. We have shown that the stress-related and anxiogenic neuropeptide corticotropin-releasing factor can stimulate the in vitro neuronal firing rates of topographically organized subpopulations of serotonergic neurons within the dorsal raphe nucleus (DR). These findings are consistent with a wealth of behavioral studies suggesting that serotonergic systems within the DR are involved in the modulation of ongoing anxiety-related behavior and in behavioral sensitization, a process whereby anxiety-and fear-related behavioral responses are sensitized for a period of up to 24 to 48 h. The dorsomedial subdivision of the DR, particularly its middle and caudal aspects, has attracted considerable attention as a region that may play a critical role in the regulation of acute and chronic anxiety states. Future studies aimed at characterization of the molecular and cellular properties of topographically organized subpopulations of serotonergic neurons are likely to lead to major advances in our understanding of the role of serotonergic systems in stress-related physiology and behavior.

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Distribution of c-Fos in guinea-pig brain following morphine withdrawal

Cited by 17 publications

References 21 publications

The anabolic androgenic steroid, nandrolone decanoate, increases the density of Fos‐like immunoreactive neurons in limbic regions of guinea‐pig brain

The anabolic androgenic steroid, nandrolone decanoate, increases the density of Fos‐like immunoreactive neurons in limbic regions of guinea‐pig brain

Mapping of c‐fos gene expression in the brain during morphine dependence and precipitated withdrawal, and phenotypic identification of the striatal neurons involved

Anatomic and Functional Topography of the Dorsal Raphe Nucleus

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