Distribution of blaTEM, blaSHV and blaCTX-M Genes Among Escherichia coli Isolates Causing Urinary Tract Infection in Children

Abstract

Background: Emerging antibacterial resistance rates and extended-spectrum-β-lactamase producing Escherichia coli recovered from urinary tract infections (UTI) is an increasing problem in different regions, limiting therapeutic options. Objectives: The aim of this study was the detection of bla TEM , bla SHV and bla CTX-M genes among Escherichia coli strains causing Urinary Tract Infection in children from Iran. Materials and Methods: A total of 100 Escherichia coli isolates were collected from urine specimens of patients with UTI who had referred to the Children Medical Center, Tehran, Iran between November 2012 and July 2013. Antibiotic susceptibility test was performed by Kirby-Bauer disc diffusion method according to CLSI guidelines. The bla CTX-M , bla TEM and bla SHV genes were detected by PCR and sequencing methods. Results: The resistance rate of isolates to cefotaxime, ceftazidime, imipenem, gentamicin, amikacin, cefepime, piperacillin/tazobactam, co-trimoxazole, nitrofurantoin, cefixime and cephalothin, were 51 (51%), 24 (24%), 0 (0%), 25 (25%), 2 (2%), 6 (6%), 2 (2%), 79 (79%), 7 (7%), 36 (36%) and 43 (43%), respectively. In this study, imipenem was more active than other antibiotics. The existence of bla TEM-1 and bla CTX-M-15 was detected in 69 (69%) and 74 (74%) isolates respectively, while bla SHV gene was not detected. Conclusions: The prevalence of β-lactamase-producing genes in E. coli detected by this study is of great concern and highlights the need of infection control measures including antibacterial management and prompt identification of isolates with beta-lactamase-producing genes.

Detection of pap, sfa, afa, foc, and fim Adhesin-Encoding Operons in Uropathogenic Escherichia coli Isolates Collected From Patients With Urinary Tract Infection

“…Several virulence determinants contribute to the pathogenicity of E. coli in UTI ( 17 , 19 , 27 ). They are the product of different genes, which can be detected by PCR method ( 9 , 22 ). However, there is always the possibility of mutation at the level of the corresponding gene, leading to the absence of its detection.…”

“…One hundred non-duplicate E. coli isolates were recovered by urine culture from 250 consecutive adult patients with community-acquired pyelonephritis or cystitis over a period ranging from April to September 2013 in Zabol, Iran. The isolation and identification of E. coli isolates were performed by standard bacteriological and biochemical tests using Gram staining, catalase test, indole, methyl red, Voges-Proskauer test, nitrate reduction, urease production, Simmons citrate agar, and various sugar fermentation tests ( 20 - 22 ). The bacteria were maintained in Tryptic Soy Broth (TSB) (Sigma; The USA) with Glycerol at -70°C.…”

Detection ofβ-Lactamases and Outer Membrane Porins amongKlebsiella pneumoniaeStrains Isolated in Iran

“…Feizabadi et al in Iran showed that 46.51% ( n = 40) of K. pneumoniae isolates were bla CTX-M positive [ 24 ]. Also, Goudarzi et al reported that 74 (74%) of Escherichia coli isolates were bla CTX-M-15 positive [ 25 ]. Reports of OXA-48-producing Enterobacteriaceae have recently dramatically increased worldwide, particularly in the Middle East and Europe.…”

Laboratory Detection of Carbapenemases in Gram-Negative Bacteria

“…Therefore, multidrug-resistant Gram-negative rods will be a serious problem if no adequate action is taken (4,5). Unfortunately, the issue of antibiotic resistant bacteria in Iran is increasing, following the rising trend of its global counterpart (6). One of the most common reasons for this trend is represented by the widespread overuse and incorrect prescribing practices.…”