Distribution of biglycan and decorin in rat dental tissue

Tenório, Davi Freitas; Santos, Marinilce Fagundes dos; Zorn, Telma Maria Tenório

doi:10.1590/s0100-879x2003000800012

Cited by 25 publications

(34 citation statements)

References 17 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This suggests that biglycan was involved in odontoblast differentiation, 16 but that its expression decreases in mature odontoblasts. 4 Consistent with these observations, our results demonstrate a weak expression of this proteoglycan in the odontoblastic layer.…”

Section: Biglycansupporting

confidence: 91%

“…The presence of these proteoglycans was detected immunohistochemically in human and animal pulp, and in dental germs under development. 4,6,16,17 The specificities of these antibodies were previously described 18 and the use of chondroitinase ABC was imperative to remove the chondroitin sulfate chains and expose the epitopes for antibodies linkage.…”

Section: B a C E F Dmentioning

confidence: 99%

“…3 Research involving animal dental tissues in initial stages of development has been conducted to determine the role of biglycan and decorin in dentinogenesis. 4 Recently, the histological conditions of the primary teeth pulp in physiological root resorption were analyzed, and the expression of proteins in the ECM of pulp tissue was described. 5 Nevertheless, there are currently no studies on the immunoexpression of biglycan and decorin in primary teeth.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Immunohistochemical expression of biglycan and decorin in the pulp tissue of human primary teeth during resorption

et al. 2013

View full text Add to dashboard Cite

Primary teeth are interesting models that can be used to study physiological and pathological processes involving cells and extracellular matrices in hard and soft tissues. This study investigated the expression and distribution of biglycan and decorin-the non-collagenous components of the extracellular matrix-in primary teeth tissue, during physiological root resorption. Thirty healthy human primary teeth were grouped together according to root length: Group I -two-thirds root length, Group II -one-third root length, and Group III -teeth with no root. The streptavidin-biotin-peroxidase immunohistochemical method was used with antibodies against the previously named antigens. The proteoglycans studied were found in the pulp and dentin extracellular matrix in all groups without any differences in the proteins, among the groups. Biglycan was observed mainly in predentin and in pulp connective tissue in the resorption area. In addition, decorin was observed mainly in pulp connective tissue, but near the resorption area. Biglycan and decorin were distributed differentially in the dental tissues. The present immunohistocytochemical data, combined with previously reported data, suggest that these proteoglycans could be involved in regulating the physiological resorption process in healthy primary teeth.

show abstract

Section: Biglycansupporting

confidence: 91%

Section: B a C E F Dmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Immunohistochemical expression of biglycan and decorin in the pulp tissue of human primary teeth during resorption

et al. 2013

View full text Add to dashboard Cite

show abstract

“…In the craniofacial region, Bgn and Dcn are present in teeth (22,28), periodontal tissues (21), nasal cartilage (29), eye (8), temporomandibular joint (18), the developing mandible (16,35) and the developing calvaria (35). Despite their prevalence in numerous tissues of the craniofacial complex, little is known about their function in these tissues.…”

Section: Introductionmentioning

confidence: 99%

Impaired posterior frontal sutural fusion in the biglycan/decorin double deficient mice

Wadhwa

Ortiz

et al. 2007

Bone

View full text Add to dashboard Cite

Biglycan (Bgn) and decorin (Dcn) are highly expressed in numerous tissues in the craniofacial complex. However, their expression and function in the cranial sutures is unknown. In order to study this, we first examined the expression of biglycan and decorin in the posterior frontal suture (PFS), which predictably fuses between 21-45 days post-natal and in the non-fusing sagittal (S) suture from wildtype (Wt) mice. Our data showed that Bgn and Dcn were expressed in both cranial sutures. We then characterized the cranial suture phenotype in Bgn deficient, Dcn deficient, Bgn/Dcn double deficient, and Wt mice. At embryonic day 18.5, alizarin red/ alcian blue staining showed that the Bgn/Dcn double deficient mice had hypomineralization of the frontal and parietal craniofacial bones. Histological analysis of adult mice (45-60 days post natal) showed that the Bgn or Dcn deficient mice had no cranial suture abnormalities and immunohistochemistry staining showed increased production of Dcn in the PFS from Bgn deficient mice. To test possible compensation of Dcn in the Bgn deficient sutures we examined the Bgn/Dcn double deficient mice and found they had impaired fusion of the PFS. Semi-quantitative RT-PCR analysis of RNA from 35 day-old mice revealed increased expression of Bmp-4 and Dlx-5 in the PFS compared to their non-fusing S suture in Wt tissues and decreased expression of Dlx-5 in both PF and S sutures in the Bgn/Dcn double deficient mice compared to the Wt mice. Failure of PFS fusion and hypomineralization of the calvaria in the Bgn/Dcn double deficient mice demonstrate these extracellular matrix proteoglycans could have a role in controlling the formation and growth of the cranial vault.

show abstract

“…Others have applied the roles of biglycan, and subsequent amelogenin expression, to nanofiber scaffolding to study enamel maturation [99][100][101]. Mouse knockout models have also elucidated the role of Tbx1 and enamel formation [102].…”

Section: Whole-tooth Engineeringmentioning

confidence: 99%