Distribution of bacteria and nitrogen-cycling microbial communities along constructed Technosol depth-profiles

Hafeez, Farhan; Spor, Aymé; Breuil, Marie-Christine; Schwartz, Christophe; Martin-Laurent, Fabrice; Philippot, Laurent

doi:10.1016/j.jhazmat.2012.06.041

Cited by 31 publications

(18 citation statements)

References 63 publications

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“…Although they are intensively managed, and despite their artificial nature, we have shown that the soils of the Eden Project house communities of invertebrates and microbes that contribute to organic matter decomposition. This strengthens evidence that technosols are capable of supporting a functioning microbial community (Hafeez et al ). Our results support the view that a wide range of ecosystem processes depend upon communities across multiple trophic levels (Soliveres et al ).…”

Section: Discussionsupporting

confidence: 88%

The relative importance of invertebrate and microbial decomposition in a rainforest restoration project

Donald

Weir

Bonnett

et al. 2017

Restoration Ecology

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Tropical rainforests are increasingly disturbed by human activities. While restoration projects often succeed in replacing tree cover, they rarely manage to restore soil function. Consequently, there is an urgent need to understand the changes that occur during soil restoration. Model ecosystems such as the Eden Project present an ideal opportunity to investigate these changes. The Eden Project was built 15 years ago, and its plants grown from seedlings, or sown directly into a soil made up of standardized mixtures of recycled organic material. Today, the Eden Project's rainforest biome consists of a diverse community of plants, invertebrates, and microorganisms. Different areas within the biome are managed differently, allowing us to separate the relative contributions of decomposers under differing physical conditions. Litterbag experiments revealed significant differences in decomposition rates in bags of different mesh sizes. Phospholipid fatty acid analysis revealed that microbial biomass and community structure varied under different management regimes. Soil enzyme assays revealed that glucosidase activity increased in soils with more organic matter, whereas phenol oxidase activity increased in more alkaline soils. Our study takes a step toward understanding the interactions between invertebrates and microbes, and the way in which soils function during restoration.

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Section: Discussionsupporting

confidence: 88%

The relative importance of invertebrate and microbial decomposition in a rainforest restoration project

Donald

Weir

Bonnett

et al. 2017

Restoration Ecology

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“…Recently, it was suggested that following ex situ treatments, treated soil can be used to construct a new soil profile at the polluted site, named Technosol, made of treated soil, recycled waste, and industrial by-products (Sere et al 2008;WRB IWG 2006). Mainly applied to treat PAHs and heavy metal contamination, it was shown that the abundance and the activity of N-cycling microbial guilds in Technosols were in the same range as in the other terrestrial ecosystems suggesting that construction of Technosols is a promising technology for the restoration of degraded lands and recycling of industrial wastes (Hafeez et al 2012). It has to be noticed that during the past two decades, on-farm bioremediation devices designed to effluents of pesticides have been developed and are now on the market being recognized by the authorities as one of the solution to treat pesticides effluents (for review, see De Wilde et al 2007).…”

Section: Pesticide Bioremediationmentioning

confidence: 99%

Applied Microbial Ecology and Bioremediation

Bertrand

Doumenq²,

Guyoneaud

et al. 2014

Environmental Microbiology: Fundamentals and Applications

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The large diversity of metabolic capacities and the high genetic plasticity of microorganisms allow them to degrade virtually all organic compounds of natural or anthropogenic (xenobiotics) origin including those that are sources of environmental pollution. Thus microorganisms are major actors to eliminate or alleviate pollutions in the environment. The natural attenuation processes due to microbial activities (biodegradation and/or biotransformation) as well as the possibilities of using microorganisms in preventive treatments and bioremediation -biostimulation, bioaugmentation, rhizostimulation, bioleaching, and bioimmobilization -are presented. The main methods for microbial treatment of pollution, the chemical structure and the origin of the major pollutants, as well as the mechanisms of degradation by microorganisms -on the basis of physiological, biochemical, and genetic approaches -are described. Examples of treatments are presented for urban wastewater (activated sludge, lagoons, and planted beds), solid wastes (aerobic treatment or composting, anaerobic treatment and methanization, discharges), gaseous effluents, pesticides, polychlorobiphenyls, and finally hydrocarbons and petroleum products in the marine environment.

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“…The method has been unanimously approved by the ISO as an international standard method (ISO standard 11063) [80]. The method has also been used to extract DNA from river sediments and agricultural soil, waters, biofilms and glacier soils [10,11], polychlorinated biphenyls-contaminated sites [79], constructed wetlands [21], and technosols [40]. Essentially, the method is as follows.…”

Section: Initial Attempts For Dna Isolationmentioning

confidence: 99%

“…Primers used for amplification are presented in Table 1. Those primers have been used for amplification of the 16S rRNA and denitrification genes from agricultural soils [1,5,23,62,72], forest soils [4], containers filled with wood byproducts [128], rice paddy field soils [134], grassland pasture soils [82], soils from the Burgundy region [10], soil-feeding mounds of nematodes [28], technosols [40], and constructed wetlands [21].…”

Section: Preparation Of Standard Dnas For Qpcrmentioning

confidence: 99%

Determination of Denitrification Genes Abundance in Environmental Samples

Correa‐Galeote¹,

Tortosa²,

Bedmar³

2013

Metagenomics

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Diversity of microorganisms involved in the biogeochemical N cycle is of fundamental interest in microbial ecology. Denitrification is a key step in the cycle by which nitrate is reduced to dinitrogen gas via the soluble nitrite and the gaseous compounds nitric oxide and nitrous oxide. The process is carried out by the sequential activity of the nitrate, nitrite, nitric oxide, and nitrous oxide reductase enzyme, respectively. The fluorescence-based quantitative real-time polymerase chain reaction (qPCR) is widely used for quantification of nucleic acids in samples obtained from numerous, diverse sources. Here, we provide a well-proven methodology for isolation of DNA from environmental samples and describe relevant experimental conditions for utilization of qPCR to assay the 16S rRNA and nar/nap, nirK/nirS, c-nor/qnor, and nos denitrification genes that encode synthesis of denitrifying enzymes. The ISO 11063 standard method and MIQUE guidelines are considered with the aim to increase experimental transparency.

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Distribution of bacteria and nitrogen-cycling microbial communities along constructed Technosol depth-profiles

Cited by 31 publications

References 63 publications

The relative importance of invertebrate and microbial decomposition in a rainforest restoration project

The relative importance of invertebrate and microbial decomposition in a rainforest restoration project

Applied Microbial Ecology and Bioremediation

Determination of Denitrification Genes Abundance in Environmental Samples

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