Distribution of atrial natriuretic factor receptors in dog kidney fractions

Léan, André De; Vinay, Patrick; Cantin, M.

doi:10.1016/0014-5793(85)80160-5

Cited by 47 publications

(11 citation statements)

References 21 publications

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“…The difference between WKY and SHR preceded the phase of established hypertension. Thus, the fold increase of glomerular cGMP levels by ANP was 8 and 33 for WKY and SHR, respectively, at 6 wk ofage, 11 (Fig. 3) bound to the ANP C-receptor (Fig.…”

Section: Resultsmentioning

confidence: 89%

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Increased cyclic guanosine monophosphate production and overexpression of atrial natriuretic peptide A-receptor mRNA in spontaneously hypertensive rats.

Tremblay

Huot²,

Willenbrock³

et al. 1993

J. Clin. Invest.

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Atrial natriuretic peptide (ANP) specifically stimulates particulate guanylate cyclase, and cyclic guanosine monophosphate (cGMP) has been recognized as its second messenger. Spontaneously hypertensive rats (SHR) have elevated plasma ANP levels, but manifest an exaggerated natriuretic and diuretic response to exogenous ANP when compared to normotensive strains. In isolated glomeruli, the maximal cGMP response to ANP corresponds to a 12-to 14-fold increase over basal levels in normotensive strains (Wistar 13±2; Wistar-Kyoto 12+2;Sprague-Dawley 14±2) while a maximal 33±3-fold elevation occurs in SHR (P < 0.001). This hyperresponsiveness of cGMP is reproducible in intact glomeruli from SHR from various commercial sources. Furthermore, this abnormality develops early in life, even before hypertension is clearly established, and persists despite pharmacological modulation of blood pressure, indicating that it is a primary event in hypertension.In vitro studies have revealed a higher particulate guanylate cyclase activity in membranes from glomeruli and other tissues from SHR. This increase is not accounted for by different patterns of ANP binding to its receptor subtypes between normotensive and hypertensive strains, as assessed by competitive displacement with C-ANP'02-121, an analog which selectively binds to one ANP receptor subtype. The hyperactivity of particulate guanylate cyclase in SHR and its behavior under basal, ligand (ANP), and detergent-enhanced conditions could be attributed either to increased expression or augmented sensitivity of the enzyme. Radiation-inactivation analysis does not evoke a disturbance in the size of regulatory elements normally repressing enzymatic activity, while the expression of particulate guanylate cyclase gene using mutated standard of A-and B-receptors partial cDNAs, quantified by polymerase chain reaction (PCR) transcript titration assay, manifests a selective increase of one guanylate cyclase subtype. Our data suggest that in hypertension, genetic overexpression of the ANP A-receptor subtype is related to the exaggerated biological response to ANP in this disease. (J. Clin. Invest. 1993. 92:2499-2508

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Section: Resultsmentioning

confidence: 89%

“…Despite numerous investigations of its renal mechanisms of action, the exact processes involved are still not fully understood. High concentrations of particulate guanylate cyclase and cGMP levels elevated by ANP have been noted in glomeruli (6)(7)(8)(9), which possess an abundance of specific ANP receptors (6,(10)(11)(12)(13)(14).…”

Section: Introductionmentioning

confidence: 99%

Increased cyclic guanosine monophosphate production and overexpression of atrial natriuretic peptide A-receptor mRNA in spontaneously hypertensive rats.

Tremblay

Huot²,

Willenbrock³

et al. 1993

J. Clin. Invest.

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“…This component of ANF's diuretic activity could be mediated via an inhibition of ADH secretion, which has been reported (45)(46)(47)(48), or via its tubular effect (49). Receptors for ANF have been described in various sections of the medulla (12,50). Our results indicate that such an anti-ADH action of ANF, whether or not related to the stimulation of particulate guanylate cyclase, is not reflected by urinary or plasma cGMP levels.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, ANF apparently inhibits tubular sodium reabsorption, especially at the level of the medullary collecting duct (9-1 1). Although no receptors have been found on proximal tubules (12,13), lithium excretion is reported to be increased, suggesting either decreased reabsorption in the proximal tubules (14) or an effect secondary to hemodynamic changes.…”

Section: Introductionmentioning

confidence: 99%

Dissociation of natriuresis and diuresis and heterogeneity of the effector system of atrial natriuretic factor in rats.

Willenbrock

Tremblay²,

García³

et al. 1989

J. Clin. Invest.

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The hypotensive, natriuretic, and diuretic actions of human atrial natriuretic factor499-126) (hANF) Met-O-ANF doses, which led to a significant increase in diuresis, were neither natriuretic nor accompanied by an increase of urinary cGMP. We were thus able to dissociate the diuretic and natriuretic effects of ANF. High doses of the oxidized analogue were required to elevate cGMP levels in plasma and urine. In isolated kidney fractions, Met-O-ANF's action on cGMP was significantly lower in glomeruli (fivefold less), virtually absent in the collecting duct, yet only slightly different (20% less) in thick ascending limb. Our results indicate that the diuretic and natriuretic effects are exerted at distinct sites, with only the natriuresis being related to an increase of extracellular cGMP. The variability of differential potency of biological and biochemical effects from tissue to tissue of these two forms of human ANF support the notion of the heterogeneity of the ANF effector system.

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“…Its natriuretic and diuretic activities are mediated by interaction with specific renal receptors. Previous studies demonstrated that two distinct receptor types are present in kidney, a high-affinity binding site (B recep tor) and a low-affinity binding site (C receptor) (7,21). It has been suggested that the Β receptor, which is tightly coupled to particulate guanylate cyclase, acts as the biologically active receptor (5), whereas C receptors seem to function as a hormonal buffer system by modulating plasma concentrations of ANF (2).…”

Section: Mcenroe and Alexander L Gerbesmentioning

confidence: 99%

Dehydration increases the density of C receptors for ANF on rat glomerular membranes

Kollenda¹,

Vollmar²,

McEnroe³

et al. 1990

American Journal of Physiology-Regulatory, Integrative and Comp

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The present study determined the presence of two types of binding sites for atrial natriuretic factor (ANF), the B and C receptor, on rat glomerular membranes. The effect of short-term salt loading and dehydration on these two receptor populations was investigated consecutively. Salt-loaded rats did not show significant changes in plasma ANF concentrations or in the number of ANF binding sites. Water-deprived rats presented significantly lower plasma ANF concentrations (22.0 +/- 1.9 vs. 34.4 +/- 3.8 fmol/ml, P less than 0.01) and an increase in total receptor density (1,860 +/- 398 vs. 987 +/- 143 fmol/mg protein) as compared with the control group. Differentiation of both receptor populations showed that it was the C receptors that accounted for this increase (1,772 +/- 369 vs. 901 +/- 151 fmol/mg protein, P less than 0.05), whereas B-receptor density was unchanged (89 +/- 31 vs. 87 +/- 44 fmol/mg protein). These data suggest that C receptors for ANF are affected by changes of body fluid volume.

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Distribution of atrial natriuretic factor receptors in dog kidney fractions

Cited by 47 publications

References 21 publications

Increased cyclic guanosine monophosphate production and overexpression of atrial natriuretic peptide A-receptor mRNA in spontaneously hypertensive rats.

Increased cyclic guanosine monophosphate production and overexpression of atrial natriuretic peptide A-receptor mRNA in spontaneously hypertensive rats.

Dissociation of natriuresis and diuresis and heterogeneity of the effector system of atrial natriuretic factor in rats.

Dehydration increases the density of C receptors for ANF on rat glomerular membranes

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