Distribution of aromatase in the brain of the African cichlid fish <scp><i>Astatotilapia burtoni</i></scp>: Aromatase expression, but not estrogen receptors, varies with female reproductive‐state

Maruska, Karen P.; Butler, Julie M.; Anselmo, Chase M.; Tandukar, Ganga

doi:10.1002/cne.24908

Cited by 24 publications

(28 citation statements)

References 102 publications

(134 reference statements)

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“…Distracting artifacts were removed with the Photoshop clone tool as needed. To facilitate the identification of neuroanatomical structures and brain nuclei, we used a cresyl violet‐stained A. burtoni reference brain and annotated atlas, as well as other brain atlases from this and other teleost species (Burmeister et al., 2009; Maruska et al., 2017; Maruska, Butler, Anselmo, et al., 2020; Muñoz‐Cueto, 2001; Wullimann et al., 1996).…”

Section: Methodsmentioning

confidence: 99%

Expression and localization of neuronal nitric oxide synthase in the brain and sensory tissues of the African cichlid fish Astatotilapia burtoni

Mobley

Ray

Maruska

2022

J of Comparative Neurology

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Nitric oxide (NO) produced by the enzyme neuronal nitric oxide synthase serves as an important neurotransmitter in the central nervous system that is involved in reproductive regulation, learning, sensory processing, and other forms of neural plasticity. Here, we map the distribution of nnos-expressing cells in the brain and retina of the cichlid fish Astatotilapia burtoni using in situ hybridization. In the brain, nnos-expressing cells are found from the olfactory bulbs to the hindbrain, including within specific nuclei involved in decision-making, sensory processing, neuroendocrine regulation, and the expression of social behaviors. In the retina, nnos-expressing cells are found in the inner nuclear layer, presumably in amacrine cells. We also used quantitative PCR to test for differences in nnos expression within the eye and olfactory bulbs of males and females of different reproductive states and social statuses. In the eye, males express more nnos than females, and socially dominant males express more nnos than subordinate males, but expression did not differ among female reproductive states. In the olfactory bulbs, dominant males had greater nnos expression than subordinate males. These results suggest a status-specific function for NO signaling in the visual and olfactory systems that may be important for sensory perception related to mating or territorial interactions to maintain the social hierarchy. The widespread distribution of nnos-expressing cells throughout the cichlid brain is similar to that in other teleosts, with some conserved localization patterns across vertebrates, suggesting diverse functions for this important neurotransmitter system.

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Section: Methodsmentioning

confidence: 99%

Expression and localization of neuronal nitric oxide synthase in the brain and sensory tissues of the African cichlid fish Astatotilapia burtoni

Mobley

Ray

Maruska

2022

J of Comparative Neurology

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“…Gravid, ready‐to‐spawn A . burtoni females have higher circulating estradiol concentrations (Butler et al, 2019; Maruska et al, 2020; Maruska & Fernald, 2010) and higher expression of aromatase (Maruska et al, 2020), an estrogenic enzyme, compared to recovering and mouthbrooding females. This, combined with the expression of estrogen receptors in both the preoptic area and ventral hypothalamus (Munchrath & Hofmann, 2010), suggest that increased estrogen signaling in the brain could mediate increases in AVT observed here.…”

Section: Discussionmentioning

confidence: 99%

“…A separate set of animals was used to examine avt mRNA levels via quantitative PCR. Gravid, brooding, and recovering females were identified from community tanks as described above in Section 2.1 and as part of another study (Maruska, Butler, Anselmo, & Tandukar, 2020). Immediately after sacrifice, the brains were quickly dissected from the head, embedded in OCT media, frozen on dry ice, and stored at −80°C until processing.…”

Section: Methodsmentioning

confidence: 99%

“…Brains were sectioned in the transverse plane at 300 μm on a cryostat and collected onto charged slides, which were stored at −80°C until microdissection. Microdissections were done as described in (Maruska et al, 2020). Briefly, slides were placed on a frozen stage (BFS‐30MP, Physiotemp Instruments) and viewed through a dissection microscope.…”

Section: Methodsmentioning

confidence: 99%

“…Quantitative RT‐PCR was then used to measure mRNA levels of avt and two reference genes ( gapdh and eef1a ). qRT‐PCR was done as previously described (Butler et al, 2019; Maruska et al, 2020) with samples run in 20 μl duplicates on a CFX Connect System (BioRad). The same primer sets were used for qRT‐PCR and ISH probe generation, just without the T3 initiation sequence.…”

Section: Methodsmentioning

confidence: 99%

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Female reproductive state is associated with changes in distinct arginine vasotocin cell types in the preoptic area of Astatotilapia burtoni

Butler

Anselmo

Maruska

2020

J of Comparative Neurology

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Nonapeptides play a crucial role in mediating reproduction, aggression, and parental care across taxa. In fishes, arginine vasotocin (AVT) expression is related to social and/or reproductive status in most male fishes studied to date, and is linked to territorial defense, paternal care, and courtship. Despite a plethora of studies examining AVT in male fishes, relatively little is known about how AVT expression varies with female reproductive state or its role in female social behaviors. We used multiple methods for examining the AVT system in female African cichlid fish Astatotilapia burtoni, including immunohistochemistry for AVT, in situ hybridization for avt‐mRNA, and quantitative PCR. Ovulated and mouthbrooding females had similar numbers of parvocellular, magnocellular, and gigantocellular AVT cells in the preoptic area. However, ovulated females had larger magnocellular and gigantocellular cells compared to mouthbrooding females, and gigantocellular AVT cell size correlated with the number of days brooding, such that late‐stage brooding females had larger AVT cells than mid‐stage brooding females. In addition, we found that ventral hypothalamic cells were more prominent in females compared to males, and were larger in mouthbrooding compared to ovulated females, suggesting a role in maternal care. Together, these data indicate that AVT neurons change across the reproductive cycle in female fishes, similar to that seen in males. These data on females complement studies in male A. burtoni, providing a comprehensive picture of the regulation and potential function of different AVT cell types in reproduction and social behaviors in both sexes.

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Expression characteristics of the cyp19a1b aromatase gene and its response to 17β-estradiol treatment in largemouth bass (Micropterus salmoides)

Zhang,

Tian,

Han

et al. 2024

Fish Physiol Biochem

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To investigate the regulatory role of the Cyp19a1b aromatase gene in the sexual differentiation of largemouth bass (Micropterus salmoides, LMB), we obtained the full-length cDNA sequence of Cyp19a1b using rapid ampli cation of cDNA ends technique. Tissue expression characteristics and feedback with 17-β-estradiol (E 2 ) were determined using quantitative real-time PCR (qRT-PCR), while gonad development was assessed through histological section observations. The cDNA sequence of LMB Cyp19a1b was found to be1950 base pairs (bp) in length, including a 5' untranslated region of 145 bp, a 3' untranslated region of 278 bp, and an open reading frame encoding a protein consisting of 1527 bp that encoded 508 amino acids. The qRT-PCR results indicated that Cyp19a1b abundantly expressed in the brain, followed by the gonads, and its expression in the ovaries was signi cantly higher than that observed in the testes.(P < 0.05). After feeding sh with E 2 for 30 days, the expression of Cyp19a1b in the pseudo-females gonads (XY-F) was signi cantly higher than that in males (XY-M) (P < 0.05), whereas expression did not differ signi cantly between female (XX-F) and XY-M sh (P > 0.05). Although the expression of Cyp19a1b in XY-F and XX-F sh was not signi cantly different after 60 days (P > 0.05), both exhibited signi cantly higher levels than that of XY-M sh (P < 0.05). Histological sections analysis showed the presence of oogonia in both XY-F and XX-F sh at 30 days, while spermatogonia were observed in XY-M sh. At 60 days, primary oocytes were abundantly observed in both XY-F and XX-F sh, while a few spermatogonia were visible in XY-M sh. At 90 days, the histological sections results showed that a large number of oocytes were visible in XY-F and XX-F sh. Additionally, the gonads of XY-M sh contained numerous spermatocytes. These results suggest that Cyp19a1b plays a pivotal role in the development of ovaries and nervous system development in LMB.

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Distribution of aromatase in the brain of the African cichlid fish Astatotilapia burtoni: Aromatase expression, but not estrogen receptors, varies with female reproductive‐state

Abstract: This is the author manuscript accepted for publication and has undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as

Cited by 24 publications

References 102 publications

Expression and localization of neuronal nitric oxide synthase in the brain and sensory tissues of the African cichlid fish Astatotilapia burtoni

Expression and localization of neuronal nitric oxide synthase in the brain and sensory tissues of the African cichlid fish Astatotilapia burtoni

Female reproductive state is associated with changes in distinct arginine vasotocin cell types in the preoptic area of Astatotilapia burtoni

Expression characteristics of the cyp19a1b aromatase gene and its response to 17β-estradiol treatment in largemouth bass (Micropterus salmoides)

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