Distribution of apolipoprotein E in the plasma of insulin-dependent and noninsulin-dependent diabetics and its relation to cholesterol net transport.

Fielding, C J; Castro, Graciela; Donner, C. C.; Fielding, Phoebe E.; Reaven, Gerald M.

doi:10.1016/s0022-2275(20)38764-2

Cited by 25 publications

(2 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As far as the HDL-sized RLP are concerned, we have previously proposed that these particles represent apoEcontaining HDL-sized lipoproteins that do not contain apoB or apoA-I (9). The presence in plasma of apoEcontaining HDL devoid of apoA-I has been described by a number of authors (26,(47)(48)(49). Direct evidence for the presence of these lipoproteins in the RLP fraction of normolipidemic subjects has been provided in the present study by results of two-dimensional gel electrophoretic analysis of plasma RLP fractions (Fig.…”

Section: Discussionsupporting

confidence: 82%

Characterization of remnant-like particles isolated by immunoaffinity gel from the plasma of type III and type IV hyperlipoproteinemic patients

Marcoux

Tremblay

Nakajima

et al. 1999

Journal of Lipid Research

View full text Add to dashboard Cite

Previous studies have investigated the potential atherogenicity and thrombogenicity of triglyceride-rich lipoprotein (TRL) remnants by isolating them from plasma within a remnant-like particle (RLP) fraction, using an immunoaffinity gel containing specific anti-apoB-100 and anti-apoA-I antibodies. In order to characterize lipoproteins in this RLP fraction and to determine to what extent their composition varies from one individual to another, we have used automated gel filtration chromatography to determine the size heterogeneity of RLP isolated from normolipidemic control subjects (n ‫؍‬ 8), and from type III (n ‫؍‬ 6) and type IV (n ‫؍‬ 9) hyperlipoproteinemic patients, who by selection had similarly elevated levels of plasma triglyceride (406 ؎ 43 and 397 ؎ 35 mg/dl, respectively). Plasma RLP triglyceride, cholesterol, apoB, apoC-III, and apoE concentrations were elevated 2-to 6-fold ( P Ͻ 0.05) in hyperlipoproteinemic patients compared to controls. RLP fractions of type III patients were enriched in cholesterol and apoE compared to those of type IV patients, and RLP of type IV patients were enriched in triglyceride and apoC-III relative to those of normolipidemic subjects. In normolipidemic subjects, the majority of RLP had a size similar to LDL or HDL. The RLP of hyperlipoproteinemic patients were, however, larger and were similar in size to TRL, or were intermediate in size (i.e., ISL) between that of TRL and LDL. Compared to controls, ISL in the RLP fraction of type III patients were enriched in apoE relative to apoC-III, whereas in type IV patients they were enriched in apoC-III relative to apoE. These results demonstrate that: 1 ) RLP are heterogeneous in size and composition in both normolipidemic and hypertriglyceridemic subjects, and 2 ) the apoE and apoC-III composition of RLP is different in type III compared to type IV hyperlipoproteinemic patients. -Marcoux, C., M. Tremblay, K. Nakajima, J. Davignon, and J. S. Cohn. Characterization of remnant-like particles isolated by immunoaffinity gel from the plasma of type III and type IV hyperlipoproteinemic patients.

show abstract

Section: Discussionsupporting

confidence: 82%

Characterization of remnant-like particles isolated by immunoaffinity gel from the plasma of type III and type IV hyperlipoproteinemic patients

Marcoux

Tremblay

Nakajima

et al. 1999

Journal of Lipid Research

View full text Add to dashboard Cite

show abstract

“…Fielding and associates have reported elevated concentrations of apoE in a lipoprotein with an estimated density resembling LDL in plasma of patients with type II diabetes mellitus (36). This lipoprotein, which also contained apoB-100, was enriched in TG as compared with total LDL, thus resembling JI-H unbound IDL/LDL.…”

Section: Discussionmentioning

confidence: 93%

Properties of triglyceride-rich and cholesterol-rich lipoproteins in the remnant-like particle fraction of human blood plasma

Campos

Kotite

Blanche

et al. 2002

Journal of Lipid Research

View full text Add to dashboard Cite

An immunoassay procedure that quantifies remnant-like particle (RLP) cholesterol in human blood plasma has shown considerable promise as a clinically applicable risk marker for atherosclerotic disease. The lipoproteins included in this assay include not only certain TG-rich lipoproteins [all particles containing apolipoprotein B-48 (apoB-48) and a fraction of those containing apoB-100] but also a very small proportion of plasma cholesterol-rich lipoproteins. The TG-rich lipoprotein component of RLP has been partially characterized, but relatively little is known about the component cholesterol-rich lipoproteins. We have further characterized the properties of the TG-rich component that is included in RLP in which about 25% of the particles contain apoB-48 and the remainder apoB-100. We show that the cholesterol-rich component is comprised mainly of ␤ -migrating LDLs that contain predominantly apoB-100. ApoE found in the LDL fraction of RLP resides on pre-␤ lipoproteins that lack apoA-I as well as apoB. The TG-rich component of RLP is responsible for increased RLP-cholesterol concentrations associated with hypertriglyceridemia. By contrast, the cholesterol-rich component is a major contributor to plasma RLP-cholesterol in individuals with low plasma TG. Our results suggest that particle heterogeneity in the RLP fraction is likely to affect the ability of RLPcholesterol concentration to predict atherosclerotic risk. RLP-cholesterol concentrations in individuals with low plasma TG may not have the same clinical significance as they do in those with hypertriglyceridemia. -Campos, E.

show abstract

A Prospective Study of Egg Consumption and Risk of Cardiovascular Disease in Men and Women

Stampfer²,

Rimm³

et al. 1999

JAMA

405

284

View full text Add to dashboard Cite

show abstract

Distribution of apolipoprotein E in the plasma of insulin-dependent and noninsulin-dependent diabetics and its relation to cholesterol net transport.

Cited by 25 publications

References 24 publications

Characterization of remnant-like particles isolated by immunoaffinity gel from the plasma of type III and type IV hyperlipoproteinemic patients

Characterization of remnant-like particles isolated by immunoaffinity gel from the plasma of type III and type IV hyperlipoproteinemic patients

Properties of triglyceride-rich and cholesterol-rich lipoproteins in the remnant-like particle fraction of human blood plasma

A Prospective Study of Egg Consumption and Risk of Cardiovascular Disease in Men and Women

Contact Info

Product

Resources

About