Distribution of antigenic oligomannosyl side chains in the cell wall mannans of several strains of Candida tropicalis

Kobayashi, Hidemitsu; Oyamada, Hiroko; Matsuda, Kyoko; Shibata, Nobuyuki; Suzuki, Shigeo

doi:10.1007/s00203-003-0550-7

Cited by 17 publications

(19 citation statements)

References 16 publications

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“…The predominant resonances for C. glabrata mnn2 mannan support the presence of backbone mannosyl repeat units without attached side chains based upon the major anomeric proton resonance at 4.91 ppm. This singlet resonance at 4.91 ppm is assigned to an anomeric proton of the α-(1,6)-linked backbone mannosyl repeat unit (-Mα1–6Mα1–6Mα-) containing no side chains (66, 67). Also, significant side chain structural fragments containing α-(1,2)-linked and possibly α-(1,3)-linked mannosyl repeat units (60, 65) are not indicated based upon the reduced level of these resonances in the 5.0–5.3 ppm spectral region.…”

Section: Resultsmentioning

confidence: 99%

Differential Virulence of Candida glabrata Glycosylation Mutants

West

Lowman

Mora-Montes

et al. 2013

Journal of Biological Chemistry

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Background: Candida glabrata virulence is poorly understood at the molecular level.Results: Inactivation of components of the C. glabrata glycosylation machinery results in changes in fungal mannan structure and altered virulence.Conclusion: Changes in C. glabrata cell wall architecture impact the host-pathogen interactions.Significance: Greater understanding of C. glabrata virulence will provide insights that can be adopted for development of novel diagnostic and therapeutic interventions.

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Section: Resultsmentioning

confidence: 99%

Differential Virulence of Candida glabrata Glycosylation Mutants

West

Lowman

Mora-Montes

et al. 2013

Journal of Biological Chemistry

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“…Okawa et al, 1994Okawa et al, , 1996Okawa et al, , 2005. Moreover, the disappearance of signals of 4.767 and 5.165 ppm shows that Ct-0589-37-M did not contain the manno-oligosaccharides containing both the b-1,2 and a-1,2 linkages, which correspond to the specific antigenic factor 6 (Kobayashi et al, , 2003. The a-1,6-branched mannopyranose unit, at 5.232 ppm, of Candida mannan functions as the determinant of the antigenic factor 4 (Shibata et al, 1995;Kobayashi et al, 1997Kobayashi et al, , 2003.…”

Section: Nmr Patterns Of the Mannansmentioning

confidence: 93%

Antigenicity ofCandida tropicalisstrain cells cultured at 27 and 37Â°C

Okawa

Goto

2006

FEMS Immunology &Amp; Medical Microbiology

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All cells of four Candida tropicalis strains IFO 0199 (Ct-0199), IFO 0587 (Ct-0587), IFO 1400 (Ct-1400), and IFO 1647 (Ct-1647), obtained by cultivation at 27 and 37 degrees C for 48 h in yeast extract-added Sabouraud liquid medium, showed the shapes of typical budding yeast and the same agglutination patterns against factor sera 1, 4, 5 and 6 in the commercially available kit 'Candida Check'. The cells of the C. tropicalis IFO 0589 strain display the same properties at 27 degrees C but formed hyphae at 37 degrees C. The cell wall mannan (Ct-0589-37-M) obtained from the strain cells cultured at 37 degrees C had lost most of its reactivity against factor sera 4, 5 and 6 in an enzyme-linked immunosorbent assay, in contrast to the mannan (Ct-0589-27-M) at 27 degrees C. The 1H-nuclear magnetic resonance patterns of the mannans obtained from the cells of the four C. tropicalis strains IFO 0199, IFO 0587, IFO 1400, and IFO 1647, obtained by cultivation at 37 degrees C, did not change compared to those at 27 degrees C. By contrast, the Ct-0589-37-M had significantly lost the beta-1,2-linked mannopyranose units, corresponding to the serum factors 5 and 6. These results show that the IFO 0589 strain is an unusual strain among the general C. tropicalis strains studied.

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“…The cell walls of Candida species are composed of mannan with fatty acids (Kappeli and Fiechter, 1977;Kappeli et al, 1978;Kobayashi et al, 2003;Shinoda et al, 1981). Adsorption of moisture on the cell walls of Candida may be weaker than that of D. hansenii and S. cerevisiae, whose cell walls are composed of glucan and mannoprotein (Nguyen et al, 1998).…”

Section: Survival Rates Depend On Cell Wall Structurementioning

confidence: 99%

Survival of yeasts stored after freeze-drying or liquid-drying

Miyamoto‐Shinohara¹,

Nozawa²,

Sukenobe³

et al. 2010

J. Gen. Appl. Microbiol.

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IntroductionFreeze-drying and L-drying are popular methods of preserving microorganisms, because long-term viability is excellent in most cases and the storage and distribution requirements are simple. By plotting the survival curves of freeze-dried species sealed under high vacuum ( 1 Pa) and stored at 5 C in the dark, we previously found that the survival rates of Gram-positive bacteria immediately after freeze-drying and during storage were higher than those of Gram-negative bacteria (Miyamoto-Shinohara et al., 2008). In addition, the survival rate of the yeast Saccharomyces cerevisiae immediately after freeze-drying was lower than those of bacterial species, although S. cerevisiae survival rates were stable during storage (Miyamoto-Shinohara et al., 2000.To increase the survival rates of S. cerevisiae strains, we tested the applicability of L-drying, in which cells are desiccated by vacuum evaporation at room temperature. In contrast, during freeze-drying, cells are desiccated by sublimation. L-drying is effective for the long-term preservation of cultures sensitive to freezedrying (Malik, 1990(Malik, , 1999. In this research, yeast strains were L-dried according to the method of the Institute for Fermentation, Osaka (Banno et al., 1979), in which the cell suspension was dispensed into a glass ampoule (in the same way as for freeze-drying) and a cotton wool plug was inserted to prevent the temperature in the ampoule from falling to the freezing point (Iijima and Sakane, 1973).To clarify the mechanisms underlying tolerance to L-drying and freeze-drying, we investigated the survival of type strains of various yeast species. The survival J. Gen. Appl. Microbiol., 56, 107 119 (2010) We investigated

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Distribution of antigenic oligomannosyl side chains in the cell wall mannans of several strains of Candida tropicalis

Cited by 17 publications

References 16 publications

Differential Virulence of Candida glabrata Glycosylation Mutants

Differential Virulence of Candida glabrata Glycosylation Mutants

Antigenicity ofCandida tropicalisstrain cells cultured at 27 and 37Â°C

Survival of yeasts stored after freeze-drying or liquid-drying

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