Distribution of antigen epitopes shared by nerves and the myocardium of the embryonic chick heart using different neuronal markers

Verberne, Marlies E.; Groot, Adriana C. Gittenberger‐de; Poelmann, Robert E.

doi:10.1002/1097-0185(200012)260:4<334::aid-ar20>3.0.co;2-y

Cited by 15 publications

(15 citation statements)

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“…We hypothesize that more efficient coupling of the receptors to their downstream effectors in the later developmental stages may well explain the increased sensitivity to ␤-mimetics, and we plan to perform systematic evaluation of downstream effectors (adenylate cyclase activity measurements) in future research focused on this experimental model. Moreover, it has been reported that sympathetic autonomic innervation reaches the developing chick heart after the parasympathetic (vagal) nerves at ED9 (11,28). We also observed that long-lasting effect of ␤-blocking agents lead to the downregulation of ␤-ARs specifically in early embryonic stages.…”

Section: Discussionmentioning

confidence: 99%

Heart rate changes mediate the embryotoxic effect of antiarrhythmic drugs in the chick embryo

Kočková

Svatunkova

Novotný

et al. 2013

American Journal of Physiology-Heart and Circulatory Physiology

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Kockova R, Svatunkova J, Novotny J, Hejnova L, Ostadal B, Sedmera D. Heart rate changes mediate the embryotoxic effect of antiarrhythmic drugs in the chick embryo. Am J Physiol Heart Circ Physiol 304: H895-H902, 2013. First published January 11, 2013; doi:10.1152/ajpheart.00679.2012.-A significant increase in cardiovascular medication use during pregnancy occurred in recent years. Only limited evidence on safety profiles is available, and little is known about the mechanisms of adverse effect on the fetus. We hypothesized that drug-induced bradycardia is the leading mechanism of developmental toxicity. Embryotoxicity was tested in ovo after administration of various doses of metoprolol, carvedilol, or ivabradine. Embryonic day (ED) 4 and 8 chick embryos were studied by video microscopy and ultrasound biomicroscopy ex ovo after intraamniotic injection of the drug for a period of 30 min. Stroke volume was calculated by the Simpson method and prolate ellipsoid formula. Significant dose-dependent mortality was achieved in embryos injected with carvedilol and ivabradine. In ED4 embryos, metoprolol, carvedilol, and ivabradine reduced the heart rate by 33%, 27%, and 55%, respectively, compared with controls (6%). In ED8 embryos this effect was more pronounced with a heart rate reduction by 71%, 54%, and 53%, respectively (controls, 36%). Cardiac output decreased in all tested groups but only proved significant in the metoprolol group in ED8 embryos. The number of ␤-adrenergic receptors showed a downward tendency during embryonic development. A negative chronotropic effect of metoprolol, carvedilol, and ivabradine was increasingly pronounced with embryonic maturity despite a downward trend in the number of ␤-adrenergic receptors. This effect was associated with reduced cardiac output in chick embryos, probably leading to premature death. Although standard doses of these drugs appear relatively safe, high doses have a potentially adverse effect on the fetus through reduced heart rate.

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Section: Discussionmentioning

confidence: 99%

Heart rate changes mediate the embryotoxic effect of antiarrhythmic drugs in the chick embryo

Kočková

Svatunkova

Novotný

et al. 2013

American Journal of Physiology-Heart and Circulatory Physiology

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“…Hirakow (1987), Ogawa (1952), and Shinozaki (1929-1930 also described this feature and suggested a relationship with the shift in the left ventricle from a left-lateral to ventral position. In addition, a series of embryological researches by Verberne et al (1998Verberne et al ( , 2000aVerberne et al ( , 2000b) revealed much about autonomic nerve differentiations from the neural crest using the chick embryo. In their papers, they showed a difference in distribution between the rightand left-sided cardiac neural crest cells at the arterial pole and ventral cardiac plexus, whereas the right and left cardiac neural crest cells mixed in the dorsal cardiac plexus.…”

Section: Parasympathetic Cardiac Branchesmentioning

confidence: 98%

The autonomic nervous system of the human heart with special reference to its origin, course, and peripheral distribution

2005

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A submacroscopic anatomical investigation of the entire autonomic cardiac nervous system, from origin to peripheral distribution, was performed by examining 36 sides of 18 adult human cadavers under a stereomicroscope. The following new results and points of discussion were obtained: (1) The superior cervical, the middle cervical, the vertebral, and the cervicothoracic (stellate) ganglia, composed of the inferior cervical and 1st thoracic ganglia, were mostly consistent among the specimens. (2) The superior, middle, and inferior cardiac nerves innervated the heart by simply following the descent of the great arteries. In contrast, the thoracic cardiac nerve in the posterior mediastinum followed a complex course because of the long distance to the middle mediastinum. (3) The actual course of the right thoracic cardiac nerve differed from that of the previous descriptions in that it ascended obliquely or ran transversely to the vertebrae, regardless of the intercostal vessels. Regarding the right thoracic cardiac nerve, two descending courses were observed: the descent of the right thoracic cardiac nerve via the azygos vein and right venous porta, and the descent of the recurrent right thoracic cardiac nerve via the aorta. (4) The cranial cardiac nerve and branch tended to distribute into the heart medially, and the caudal cardiac nerve and branch tended to distribute into the heart laterally. (5) The mixing positions (cardiac plexus) of the sympathetic cardiac nerve and the vagal cardiac branch, as well as the definitive morphology of brachial arteries with the recurrent laryngeal nerves, tended to differ on both sides. These new and detailed anatomical descriptions of the human autonomic cardiac nervous system may provide important clues regarding the morphogenesis of autonomic cardiac nerves in addition to contributing to the improvement of cardiac surgery.

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“…1C). HNK-1 antibody recognizes a complex carbohydrate moiety on the cell surface and has been used extensively as a marker for the ventricular conduction system in many species including chick, rat, rabbit and human (Aoyama et al, 1993;Aoyama et al, 1995;Chuck and Watanabe, 1997;Gorza et al, 1988;Ikeda et al, 1990;Luider et al, 1993;Nakagawa et al, 1993;Nakamura et al, 1994;Sakai et al, 1994;Verberne et al, 2000), and overlaps with another conduction system marker, Cx40 ( Supplementary Fig. 1S).…”

Section: Resultsmentioning

confidence: 97%

“…Antibodies to Leu-7 (HNK-1) have been used widely to delineate the developing conduction system in mammals and in chicken (Chuck and Watanabe, 1997;Gorza et al, 1988;Moorman et al, 1998;Nakagawa et al, 1993;Verberne et al, 2000). The HNK-1 antibody recognizes a complex sulfate-3-glucuronyl carbohydrate moiety, which is present on a series of molecules involved in cell adhesion and extracellular matrix interaction.…”

Section: Introductionmentioning

confidence: 99%

“…The HNK-1 antibody recognizes a complex sulfate-3-glucuronyl carbohydrate moiety, which is present on a series of molecules involved in cell adhesion and extracellular matrix interaction. The antibody to SNAP-25 protein, a component of the SNARE complex, has also been used to mark the elements of the ventricular conduction system in chick (Verberne et al, 2000). Among the gap junction protein connexins, Connexin40 (Cx40) or the chicken homolog Cx42, has been used as a marker for the conduction system in many species (Bastide et al, 1993;Delorme et al, 1997;Gourdie et al, 1993b;Gros et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Notch signaling plays a key role in cardiac cell differentiation

Chau

Tuft

Fogarty

et al. 2006

Mechanisms of Development

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Results from lineage tracing studies indicate that precursor cells in the ventricles give rise to both cardiac muscle and conduction cells. Cardiac conduction cells are specialized cells responsible for orchestrating the rhythmic contractions of the heart. Here, we show that Notch signaling plays an important role in the differentiation of cardiac muscle and conduction cell lineages in the ventricles. Notch1 expression coincides with a conduction marker, HNK-1, at early stages. Misexpression of constitutively active Notch1 (NIC) in early heart tubes in chick exhibited multiple effects on cardiac cell differentiation. Cells expressing NIC had a significant decrease in expression of cardiac muscle markers, but an increase in expression of conduction cell markers, HNK-1, and SNAP-25. However, the expression of the conduction marker connexin 40 was inhibited. Loss-of-function study, using a dominant-negative form of Suppressor-of-Hairless, further supports that Notch1 signaling is important for the differentiation of these cardiac cell types. Functional studies show that the expression of constitutively active Notch1 resulted in abnormalities in ventricular conduction pathway patterns.

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Distribution of antigen epitopes shared by nerves and the myocardium of the embryonic chick heart using different neuronal markers

Cited by 15 publications

References 70 publications

Heart rate changes mediate the embryotoxic effect of antiarrhythmic drugs in the chick embryo

Heart rate changes mediate the embryotoxic effect of antiarrhythmic drugs in the chick embryo

The autonomic nervous system of the human heart with special reference to its origin, course, and peripheral distribution

Notch signaling plays a key role in cardiac cell differentiation

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