Distribution of <i>Candida</i> Species and Molecular Typing of <i>C. albicans</i> Isolates in a Mexico City Tertiary Care Hospital from 2011 to 2013

Abstract

The incidence of yeast infections has increased significantly over the past few years, constituting a leading cause of morbidity and mortality among hospitalised patients. The rapid identification of candidiasis is important for the clinical management of patients and to facilitate tracing the sources of infections in hospitalized patients. Here, we report a retrospective, single-centre study of Candida spp. distribution and antifungal susceptibility from January 2011 to May 2013 at a hospital in México City, regarding the importance of elucidating the identity of the infection-causing Candida species in order to improve prophylactic measures and treatment. Clinical data were collected from patient medical records and the laboratory database. Isolates were initially identified using standard mycology techniques, and then confirmed by PCR-based system using amplification of intergenic spacers (rDNA ITS) and restriction length polymorphism of PCR products after sequence-specific enzymatic cleavage (PCR-RFLP). We observed no shift from C. albicans to nonalbicans Candida species: Candida albicans (73.7%) was the most prevalent species isolated, while C. dubliniensis was not identified in this study. Antifungal susceptibility was determined using FUNGITEST ® ; 17.4% of C. albicans isolates were resistant to fluconazole and 21.7% to itraconazole. Multiplex PCR microsatellite analysis of the clinical C. albicans isolates using primers for the CAI, CAIII and CAVI loci identified 29 different alleles for CAI, 8 alleles for CAIII and 31 for CAVI. The combined discriminatory power of these three microsatellites was 0.98, which was considered reliable for molecular typing. Genetic analysis of these isolates revealed a clonal population with a total of 62 genotypes from the examined isolates.

Daya Hambat Virgin Coconut Oil Terhadap Pertumbuhan Jamur Candida Albicans Yang Diisolasi Dari Sampel Swab Vagina

“…Jamur kemudian berkolonisasi pada berbagai bagian tubuh terutama pada rongga mulut, usus, vagina dan menjadi komensal (Miranda et al 2009). Hasil indetifikasi molekuler menunjukkan C. albicans merupakan jenis yang ditemukan paling banyak pada tubuh manusia (73 %), diikuti C. tropicalis (15.0%), C. glabrata, (3.6%), dan C. parapsilosis (5.4% (Guerrero et al, 2016) Jamur Candida memiliki faktor adhesi yang membantu kolonisasi pada berbagai bagian tubuh tersebut dan juga berperan dalam patogenesis jamur. Berbagai molekul protein dapat disekresikan ke permukaan sel dan membantu pelekatan Candida para sel mukosa seperti protein famili Als, Hwp1, dan Eap1.…”

Emerging Pathogens of the Candida Species

“…In addition, detection of microsatellites also represents a reliable method for molecular typing and genetic analysis of Candida. A recent Candida distribution study conducted in a hospital, reported a clonal population including 62 identified genotypes among the tested isolates [25]. Beyond that, multilocus sequence typing (MLST) is another valuable method to understand the epidemiology of systemic Candida infections [2].…”

Prevalência e virulência de Candida spp. em indivíduos com tuberculose e suas relações com proteínas antifúngicas salivares

“…Embora presente de maneira comensal no trato digestivo e, sobretudo, na cavidade oral, alguns estudos mostraram que a colonização oral assintomática pode levar à lesão oral ou tornar-se uma fonte de infecções disseminadas (Lockhart et al, 1999;Vargas et al, 2002;Fidel et al, 2006). As infecções sistêmicas provocadas pela Candida possuem uma taxa de mortalidade de 40% (Guerrero et al, 2016).…”