DISTRIBUTION OF ALLOGENEIC 51Cr-LABELLED LYMPH NODE CELLS IN MICE

Assay for the Immunosuppressive Capacity of Antilymphocyte Serum Based on its Action on Thymus-Derived Cells

“…c) The 51Cr label technique provides a sensitive and highly repro ducible measure of the localization pattern of injected lymphoid cells [Bainbridge et al, 1966].…”

An Enterovirus-Induced Murine Model of an Acute Dilated-Type Cardiomyopathy

“…After transfusion of s,Cr-labelled lymph node cells into healthy subjects, the major organs of distribution over 48 h are the liver and spleen [Bainbridge et al, 1966]. Within lymph nodes, 65-85% of the lymphocytes are of thymus descent [Par rott and DeSousa, 1971].…”

Elimination of Allogeneic Lymphocytes by Mice

“…For many years it has been known that lymphocytes, infused intravenously, pass through the lungs and after a brief period accumulate in lymphoid organs with particular efficiency (Osogoe 1950, Farr 1951, Weisberger et al 1951, McCall et al 1955, Shorter & Bollman 1960, Bunting et al 1963, Murray & Murray 1964, Bainbridge et al 1966, whence many of them recirculate (Gowans 1967, Gowans & Knight 1964, Ford & Gowans 1969. Labelling the cells with chromium-5I (McCalletal.…”

“…1955, Shorter & Bollman I960, Bunting et al 1963) is a convenient way of quantitating their distribution in the short term. It has been shown to give reliable estimates of the proportions of injected ceils remaining viable in lymphoid tissues, and, under certain conditions, of indicating the numbers present in the liver; little label is lost from resting lymphocytes except by cell death and this is not reutilised: a little radioactivity left in debris persists in lymphoid organs when cells have died there; when cells die on entering the circulation only a small part of their radioactivity, attributable to debris, remains in the liver (Bainbridge et al 1966). Generally speaking, low levels of 5 lCr activity in the liver (less than 15 % of the injected dose) signify good viability and a high proportion of recirculating cells in the inoculum, intermediate levels (25-35%) reduced viability and cell death; high levels in the liver (40-60%) mean that large numbers of living cells have been trapped there.…”

“…After intravenous injection in mice the distribution of viable 51-chromium labelled allogeneic and syngeneic lymphocytes in lymph nodes and spleen is broadly similar over the first 48 h (Bainbridge et al 1966, Bainbridge & Gowland 1966a, although xenogeneic (rat ^ mouse, human -mouse) and syngeneic transfers show marked differences (Bainbridge, unpublished). Nevertheless mice discriminate to a limited extent against aliogeneic lymphocytes: lymph node radioactivity 18-24 h after injection ofallogeneic lymph node lymphocytes is generally lower than with syngeneic cells; an effect given the noncommittal name "natural immunity" (Bainbridge & Gowland 1966a, Pincott & Bainbridge 1980.…”