Distribution of Alleles of β-Carotene Hydroxylase 1 Gene in Modern Genotypes of Zea mays L.

SNP analysis of Ukrainian maize inbreds with alternative state of molecular carotenogenesis marker crtRB1-3’TE

“…Research material consisted of 45 maize (Zea mays L.) inbreds with perspective for maize breeding developed at the State Enterprise the Institute of Grain Crops of NAAS of Ukraine, city Dnipro (with the index DK) and three (MS252, MS381, and PHT60), by the private breeding company, the Scientifi c and Production Farming Enterprise "Company Mais" (Dnipropetrovsk region). The inbreds used by us represent common types The inbreds under investigation were divided into two groups by the allelic state of the β-carotene hydroxylase 1 gene according to the results of our previous research (Satarova et al, 2023b). The fi rst group, marked as Inb 296,296+875 , included thirty-fi ve inbreds, characterized by the allelic state of the crtRB1 gene by the crtRB1-3ʹTE marker (296 bp or 296 + 875 bp), unfavorable for the accumulation of β-carotene in the mature grain, viz: DK2, DK5, DK216, DK231, DK232, DK236/680, DK239, DK247MV, DK272, DK272/273MV, DK276, DK296, DK301, DK305, DK365, DK367, DK370, DK377, DK-517MV, DK680MV, DK742, DK744, DK777, DK959, DK2323, DK2442, DK3044, DK4173, DK4538, DKK1874, DKV3151, DKV3451, MS252, MS381and PHT60.…”

“…Very favorable for the accumulation of β-carotene in mature maize grain is a chromosomal rearrangement, which leads to blocking the enzymatic transformation of β-carotene into β-cryptoxanthin and results in the occurrence of the amplifi cation fragment of 543 bp (Yan et al, 2010;Duo et al, 2021). Among the investigated inbreds of Ukrainian selection, the ratio of genotypes with favorable (543 bp) and unfavorable (296 bp, 296 + 875 bp) allelic state of the β-carotene hydroxylase 1 gene by the crtRB1-3ʹTE marker is about 1 : 3 (Satarova et al, 2023b).…”