Distribution of Acid Phosphatase in Paramecium caudatum: Its Relations with the Process of Digestion

Esteve, Jean-Claude

doi:10.1111/j.1550-7408.1970.tb05155.x

Cited by 47 publications

(11 citation statements)

References 33 publications

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“…Hypothetical fusion sequence commencing in J with a dense, partially coated vesicle (d) in contact with the pre-trichocyst membrane (arrow), followed by fusion of the pre-trichocyst and dense vesicie membranes in the region indicated by arrowheads in K, and finally, entry of the electron-dense arm precursor material (a') into the pre-trichocyst in L. Arrows in L indicate possible shedding of dense vesicle coat material. M, nae [10] located at the trans face of the Golgi stack [7]. Thus, by analogy with what is known about lysosome formation in other cells [27], these cisternae are the functional equivalent of the TGN in P caudatum.…”

Section: The Golgi Systemmentioning

confidence: 86%

“…Thus, it will be necessary to learn where and how these lysosomes are formed before the TGN can be accurately identified in Tetrahymena. Acid phosphatase-positive vesicles, probably primary lysosomes, have been identified in the Golgi region of Paramecium caudatum [7,10] and they appear to form as bristle-coated buds by pinching off from flattened cister- The earliest pre-trichocysts identified by immunolabeling in B correspond in shape and size to the clear vesicles (c) in A. g = Golgi stack; asterisk = trans-Golgi network. C, D. The early pre-trichocyst, which in C is forming adjacent to a TGN (lower left of image) is composed of an electron-translucent vesicle containing several electron-dense arm precursors (a').…”

Section: The Golgi Systemmentioning

confidence: 99%

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Secretory organelle biogenesis: Trichocyst formation in a ciliated protozoan

Peck

Swiderski

Tourmel

1993

Biology of the Cell

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By classical electron microscopy and immunoelectron microscopy, the biogenesis of trichocyst secretory granules has been followed in the ciliated protozoan Pseudomicrothorax dubius. The very early pre-trichocysts form by fusion of bristle-coated, electron-dense vesicles (dense vesicles) with electron-translucent vesicles (clear vesicles), both of which originate in a well-developed trans-Golgi network (TGN). The pre-trichocyst grows by further fusion with dense and clear vesicles as well as with other pre-trichocysts until it reaches its maximum diameter of about 2 μm. Dense and clear vesicle formation from the TGN has been followed, and the fusion sequence of dense vesicles with the pre-trichocyst has been documented. The contents of the dense vesicles are the precursors of the trichocyst tip, which is composed of four arm-like rods, whereas the shaft precursors are supplied by the clear vesicles. The first evidence of trichocyst shaft formation is the appearance of a paracrystalline, dense core condensation center in the pre-trichocyst. Following shaft formation, the trichocyst tip forms by fusion and condensation of the dense arm precursors along each of the four sides of the shaft. Docking of the fully formed trichocyst in the cell cortex is described. Pre-trichocyst biogenesis in cells grown with and without Se is compared

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Section: The Golgi Systemmentioning

confidence: 86%

Section: The Golgi Systemmentioning

confidence: 99%

Secretory organelle biogenesis: Trichocyst formation in a ciliated protozoan

Peck

Swiderski

Tourmel

1993

Biology of the Cell

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“…1) (10,17). A DV-I is 5 6 min old, is acid phosphatase negative (9, 12,22), is surrounded initially by nonlysosomal vesicles (acidosomes) which are also devoid of acid phosphatase (8), and undergoes rapid condensation and acidification. A DV-I1 is from 4 to 10 min old, is surrounded by vesicles (lysosomes) which are acid phosphatase positive and is the most condensed and most acidic of all vacuoles.…”

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confidence: 99%

Paramecium Phagosome Membrane: From Oral Region to Cytoproct and Back Again1

Allen

1984

The Journal of Protozoology

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Ten years of research on digestive vacuoles (phagosomes) of Paramecium caudatum have revealed sequential changes both within the vacuole lumen as well as within the surrounding membrane. Four vacuole stages can be recognized by a combination of thin section and freeze-fracture ultrastructural features. Three sets of vesicles (discoidal vesicles, acidosomes, and lysosomes) fuse with the vacuole, each at a predetermined stage, to bring about these membrane and physiological changes. At various times membrane is removed as vesicles from the vacuole surface, which has the effect of regulating vacuole size. Membrane recycling, membrane replacement, and specific membrane to membrane recognition all appear to be operating during the digestive cycle. Details of these events are summarized in this address and a number of unanswered questions suggest areas for future research.HE digestive system of Paramecium has captured the in-

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“…Acid phosphatase activity was neither reported in the alveolar sacs of free living ciliates [6,121 nor detected in the alveolar sacs of I. rnultifiliis during the free living phase o f the life cycle. The vacuoles containing acid phosphatase observed in theronts must be related to the food vacuoles.…”

Section: Discussionmentioning

confidence: 88%

Enzyme Cytochemistry of the Alveolar Sacs and Golgian‐Like Cisternae in the Ciliate Ichthyophthirius multifiliis

Lobo‐da‐Cunha

Azevedo

1990

The Journal of Protozoology

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In the cell cortex of the parasitic ciliate Ichthyophthirius multifiliis different kinds of cisternae were observed: the alveolar sacs, thick membrane cisternae and the endoplasmic reticulum. The thick membrane cisternae possess coated dilated rims and sometimes could be observed close to the endoplasmic reticulum. Using cytochemical techniques acid phosphatase, thiamine pyrophosphatase and nucleoside diphosphatase activities were detected in the thick membrane cisternae and in the alveolar sacs of trophozoites. In the endoplasmic reticulum acid phosphatase activity was not detected and only very small amounts of thiamine pyrophosphatase and nucleoside diphosphatase reaction product were observed. After exit from the host, a reduction in acid phosphatase activity was evident in the alveolar sacs. At theront stage acid phosphatase activity is absent from these structures. However, high thiamine pyrophosphatase and nucleoside diphosphatase activities remain in the alveolar sacs during the whole life cycle. On the other hand, acid phosphatase, thiamine pyrophosphatase and nucleoside diphosphatase activities were detected in thick membrane cisternae of theronts. Based on the morphological aspects and enzymatic content the thick membrane cisternae of the cell cortex are designated as golgian-like cisternae. The cytochemical results point out a relationship between the alveolar sacs and the Golgi complex.

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Distribution of Acid Phosphatase in Paramecium caudatum: Its Relations with the Process of Digestion

Cited by 47 publications

References 33 publications

Secretory organelle biogenesis: Trichocyst formation in a ciliated protozoan

Secretory organelle biogenesis: Trichocyst formation in a ciliated protozoan

Paramecium Phagosome Membrane: From Oral Region to Cytoproct and Back Again1

Enzyme Cytochemistry of the Alveolar Sacs and Golgian‐Like Cisternae in the Ciliate Ichthyophthirius multifiliis

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