Distribution of 37 mucosotropic HPV types in women with cytologically normal cervical smears: The age-related patterns for high-risk and low-risk types

A Multi-Type HPV Transmission Model

“…For example, the sum of the number of individuals of a given gender, age, and sexual activity group in each compartment is verified to be equal to the total number of people N kli at each point in time (see section 3.9 on balancing population). Finally, the predictive validity of the model was evaluated by looking at age-specific HPV prevalence, CIN, genital warts, and cervical cancer incidence rates predicted by the model and comparing them with those reported in the literature (Giuliano et al, 2002;Jacobs et al, 2000;Sellors et al, 2000Sellors et al, , 2002Surveillance, Epidemiology, and End Results (SEER) Program, 2004;Insinga et al, 2003Insinga et al, , 2004b. The model predictions were well within the range of values found in the literature.…”

Detection of disseminated tumor cells in patients with cervical cancer

“…Digestion was performed in the presence of 100 lg/ml proteinase K (Sigma, Steinheim, Germany) and 0.1% Tween-20 at 56°C for 12 h. Of this suspension, 10 ll was used for PCR using consensus primers GP5+/bioGP6+as described by Jacobs et al (1995). These primers amplify a region of about 140 bp within the L1 open reading frame of more than 37 different HPV types (Jacobs et al 1997(Jacobs et al , 2000. The reaction mixture contained 50 mM KCl, 10 mM Tris-HCl (pH 8.3), 3.5 mM MgCl 2 , 200 lM of each dNTP, 1 U of thermostable DNA polymerase (AmpliTaq, Perkin-Elmer, Hu¨nenberg, Switzerland), and 50 pmol each of the GP5+ (5¢-TTTGTTACTGTGGTAGATATACTAC-3¢) and GP6+bio (5¢-GAAAAATAAACTGTAAATCATATT-3¢) primers (OliGold, Eurogentec, Maastricht, The Netherlands).…”

Liquid-based cervical cytology using ThinPrep technology: weighing the pros and cons in a cost-effectiveness analysis

“…The age distribution of the incidence of pre-invasive lesions that will regress before they become cancer was calibrated to the observed cervical intraepithelial neoplasia (CIN) detection rates in the Netherlands. The age distribution of the incidence of HPV infections that will clear before progressing to CIN was calibrated to the observed HPV prevalence [15]. Disease is subdivided into seven sequential stages: HPV infection, three pre-invasive stages (CIN 1, 2, and 3), and three invasive stages (international federation of gynaecology and obstetrics (FIGO) [16] stages 1A, 1B, and 2 ?).…”