Distribution of 2,4-Dinitrophenyl Groups on the Epidermal Langerhans Cells of Guinea Pigs following Skin Painting with 2,4-Dinitrochlorobenzene

Oka, Daisuke; Nakagawa, Shojiro; Oota, Takashi; Ueki, Hiroaki

doi:10.1159/000249286

Cited by 8 publications

(4 citation statements)

References 12 publications

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“…It has been shown by our present and previous works (3,4) that DNCB applied to animal skin enters the epidermis where it binds cell membrane components, not only of keratinocytes and Langerhans cells, but also of Thy-l + ECs. One of the important questions raised by our studies is what role these haptenated ECs play in the development of CS.…”

Section: Discussionsupporting

confidence: 52%

“…Our previous investigations (3,4), in which localization of 2,4-dinitrophenyl (DNP) groups in the skin of guinea pigs following painting the skin with 2,4--dinitrochlorobenzene (DNCB) was examined by the immunofluorescent (IF) method using antibody against DNP groups in order to clarify the mechanism of contact sensitivity (CS), showed that DNP groups were distributed on/in keratinocytes and Langerhans cells. The object of the experiments reported here was to determine whether DNP groups are also localized on Thy-I+ cells in the epidermis of mice following skin painting with DNCB.…”

Section: Introductionmentioning

confidence: 99%

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The Distribution of 2,4‐Dinitrophenyl Groups on Thy‐1 Positive Cells in the Epidermis of Mouse Following Skin Painting with 2,4‐Dinitrochlorobenzene

Bang

Nakagawa

Oka

et al. 1988

The Journal of Dermatology

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The distribution of 2,4-dinitrophenyl (DNP) groups on Thy-l positive epidermal cells in vivo after 2,4-dinitrochlorobenzene (DNCB) was painted on the skin surface of C3H/He mice was examined. Epidermal cell suspensions (EC) derived from trypsin-separated mouse ear skin painted with DNCBwere double-stained for DNP groups and Thy-l protein using FITC-anti-DNP antibody, biotinylated anti-mouse Thy-l,2 monoclonal antibody, and phyco-erythrin conjugated avidin. There were on the average 1.7% Thy-I" ECs; DNP groups were detectable on 77.8% of them when the specimen was treated with anti-DNP followed by anti-Thy-l,2 incubation. The incidence of Thy-LZ" Ee with DNP groups decreased when cells were treated with these antibodies in the reverse order. The significance of these findings is discussed in relationship to the mode ofDNCB Thy-I+ EC binding.

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Section: Discussionsupporting

confidence: 52%

Section: Introductionmentioning

confidence: 99%

The Distribution of 2,4‐Dinitrophenyl Groups on Thy‐1 Positive Cells in the Epidermis of Mouse Following Skin Painting with 2,4‐Dinitrochlorobenzene

Bang

Nakagawa

Oka

et al. 1988

The Journal of Dermatology

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“…Thus presentation by acces sory cells to T lymphocytes might occur without intracellu lar processing of the antigen. Oka et al [6] also observed an equal affinity of keratinocytes and Langerhans cells for epicutaneously applied DNCB.…”

Section: Discussionmentioning

confidence: 89%

The Hapten in Contact Hypersensitivity to Dinitrochlorobenzene: Immunoelectron Microscopic and Immunofluorescent Studies

Gawkrodger

Haftek

Botham³

et al. 1989

Dermatology

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Six hours after challenge with dinitrochlorobenzene (DNCB) in sensitized volunteers, infrequent cells reacting with both OKT6 (CD 1a) and with an antibody to DNCB were demonstrated in the upper dermis in 3 out of 4 subjects, using a double fluorescence technique. Single marker studies using polyclonal and monoclonal antibodies to DNCB showed cytoplasmic reactivity with peripheral accentuation in keratinocytes throughout the epidermis, most intense in the granular and basal layers, and occasional positive cells in the papillary dermis. Immunoperoxidase electron microscopy using polyclonal and monoclonal antibodies to DNCB demonstrated reactivity on keratinocyte and Langerhans cell membranes, with intracellular deposition of reaction product on mitochondria. Reaction product was present on the external leaflets of Birbeck granules, suggesting that the penetration of DNCB was passive rather than due to an active endocytic process. The immunofluorescent studies suggest that the double-fluorescing upper dermal cells were Langerhans cells but this could not be confirmed by electron microscopy.

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“…The epicutaneous application of DNCB is a well-established model for the study of cell-mediated immunity in both humans and laboratory animals. 4,8,22,25,26,32 Contact hypersensitivity may be considered to have two stages. There is an initial stage of sensitization resulting in the production of memory T cells and then, upon reexposure to the allergen, an elicitation phase characterized by the infiltration of the epidermis and dermis at the site of contact with mononuclear cells.…”

mentioning

confidence: 99%

Prominence of γδ T Cells in the Elicitation Phase of Dinitrochlorobenzene-induced Contact Hypersensitivity in Lambs

Jörundsson

Press

Ulvund³

et al. 1999

Vet Pathol

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Immunohistochemical techniques were used to identify T-cell subpopulations in the ear skin of lambs during the elicitation phase of dinitrochlorobenzene (DCNB)-induced contact hypersensitivity. Thirty lambs (21-26 weeks of age) were divided into groups of 10. The shaved right ear of one group was treated with DNCB. Two weeks later, this group was challenged with DNCB. One group was treated with the vehicle alone, and the remaining group was left untreated. The lambs were killed 48 hours after challenge, and tissue specimens were collected from the ears of the three groups. There was an increase in T-cell populations in the skin of the DNCB-treated lambs 48 hours after challenge. The majority of the T cells were CD8+ and associated predominantly with the blood vessels and adnexa of the superficial dermis. There was also an increased presence of CD4+ cells and gammadelta T cells in the superficial dermis. In the epidermis, clusters of gammadelta T cells and CD4+ cells were associated with microlesions. Computer-assisted morphometric analysis was used to estimate the relative presence of the T-cell subpopulations in the superficial and deep dermis and the entire dermis. Statistical analysis of the relative area of immunostaining showed that the significant increases in all T-cell subpopulations (CD4+, CD8+, and gammadelta T cells) in the entire dermis were accounted for by changes in the superficial dermis. The prominence of gammadelta T cells in delayed-type hypersensitivity in the skin of domestic ruminants has been the subject of conflicting reports. In the present study, CD4+ cell and gammadelta T-cell populations were of similar size in the normal and DNCB-treated lambs, suggesting an equal participation in the elicitation phase of contact hypersensitivity.

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Distribution of 2,4-Dinitrophenyl Groups on the Epidermal Langerhans Cells of Guinea Pigs following Skin Painting with 2,4-Dinitrochlorobenzene

Cited by 8 publications

References 12 publications

The Distribution of 2,4‐Dinitrophenyl Groups on Thy‐1 Positive Cells in the Epidermis of Mouse Following Skin Painting with 2,4‐Dinitrochlorobenzene

The Distribution of 2,4‐Dinitrophenyl Groups on Thy‐1 Positive Cells in the Epidermis of Mouse Following Skin Painting with 2,4‐Dinitrochlorobenzene

The Hapten in Contact Hypersensitivity to Dinitrochlorobenzene: Immunoelectron Microscopic and Immunofluorescent Studies

Prominence of γδ T Cells in the Elicitation Phase of Dinitrochlorobenzene-induced Contact Hypersensitivity in Lambs

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