Distribution, genetic diversity and antimicrobial resistance of clinically important bacteria from the environment of a tertiary hospital in Malaysia

Phoon, Hannah Y P; Hussin, Hazilawati; Hussain, Baizurah Mohd; Lim, Shu Yong; Woon, Jia Jie; Er, Yi Xian; Thong, Kwai Lin

doi:10.1016/j.jgar.2018.02.022

Cited by 21 publications

(17 citation statements)

References 34 publications

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“…The results obtained indicated that the prevalence of P. aeruginosa isolated from environmental samples was 20 (18.5%) which is less than that reported in other studies; (39.1%) (Okon et al, 2010) and 28% (Ndip et al, 2005). While in the study of Phoon et al, the incidence of P. aeruginosa in the environmental isolates was 5.1% (Phoon et al, 2018). This difference in prevalence rate among several studies can be attributed to differences in hygienic strategies and geographical location.…”

Section: Discussionmentioning

confidence: 91%

Molecular characterization of clinical and environmental Pseudomonas aeruginosa isolated in a burn center

Karami

Mohajeri

Mashouf

et al. 2019

Saudi Journal of Biological Sciences

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In burn centers, Pseudomonas aeruginosa acts as a major cause of nosocomial infections. Therefore, this study aimed to characterize molecularly P. aeruginosa isolates collected from environmental samples and burn patients. A total of 78 strains (including 58 clinical and 20 environmental isolates) of the P. aeruginosa were collected from Beasat hospital of Hamadan, west of Iran, and was identified using API 20NE. The disk diffusion method according to the CLSI was applied for determination of the antimicrobial resistance. Moreover, the microtiter plate test was used for the quantification of Biofilm formation. The genomic features of the isolated strains was evaluated using Pulsed Field Gel Electrophoresis (PFGE). We found that 94.8% of clinical and 80% environmental isolates were capable of forming biofilm. The rate of MDR in clinical and environmental isolates was 51.7% and 40%, respectively. A significant relationship was observed between biofilm formation capability and multiple drug resistance (p < 0.05). PFGE typing showed 11 different clusters with two major clusters A with 30 (38.5%) and B with 14 (17.9%) members, containing up to 56.4% of all isolates. There was no relationship between biofilm formation ability and antibiotic resistance patterns with PFGE patterns. According to the results, the clonal spread of environmental P. aeruginosa isolates is associated with clinical isolates, and both environmental and clinical isolates are attributed to a high prevalence of the antibiotic resistance and biofilm formation ability. This study highlighted that the prevention programs should be implemented in the hospital environment to control the spread of P. aeruginosa in burn units.

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Section: Discussionmentioning

confidence: 91%

Molecular characterization of clinical and environmental Pseudomonas aeruginosa isolated in a burn center

Karami

Mohajeri

Mashouf

et al. 2019

Saudi Journal of Biological Sciences

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“…There is a paucity of genomic resources for Enterobacteriaceae pathogens in Malaysia [1], [2], [3] despite the increasing prevalence of antibiotic resistance among members of this microbial family in the country and more broadly the Southeast Asia [4], [5]. In this data article, we generated sequencing data and assembled the draft genomes of eight extended-spectrum beta-lactamases (ESBL) producing Enterobacteriaceae bacteria isolated from a hospital in southern Malaysia.…”

Section: Datamentioning

confidence: 99%

Data on whole-genome sequencing of extended-spectrum beta-lactamases producing Enterobacteriaceae isolates from Malaysia

2019

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We report the whole genome sequencing data and de novo genome assemblies for eight extended-spectrum beta-lactamases (ESBL) producing Enterobacteriaceae isolates from Malaysia consisting of four Klebsiella pneumoniae , two Enterobacter harmaechei , one Citrobacter freundii and one Escherichia coli . We identified at least one ESBL gene in each genome, with bla CTX-M-15 being the most prevalent ESBL gene in the current genomic sampling.

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“…Therefore, the treatment of resistant bacteria is associated with hepatic and renal toxicity, long-term hospitalization of patients and significant costs incurred by the European Union’s health care system [ 85 ]. The cost associated with hospitalization and treatment of a patient infected with antibiotic-resistant bacteria is higher than 10.000 to 30.000 USD, compared to lesser costs incurred for patients treated for infection caused by β-lactams-sensitive bacteria [ 86 ]. This emerging problem is recognized by the North American and EU disease control agencies.…”

Section: The Use Of Ionic Liquids In Microbiology and Medicinementioning

confidence: 99%

“…The described mechanisms lead to antibiotic resistance to penicillins, cephalosporins, monobactams, and carbapenems, and there is an additional phenomenon of cross-resistance to macrolides and fluoroquinolones. ILs used in the treatment of general and local infections are not susceptible to either a antibiotic-resistant mechanism, which was proven in the studies on the SA1199 A and B staphylococcal strains (see Table 2 ) [ 86 , 91 , 92 , 93 ].…”

Section: The Use Of Ionic Liquids In Microbiology and Medicinementioning

confidence: 99%

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The Use of Liquids Ionic Fluids as Pharmaceutically Active Substances Helpful in Combating Nosocomial Infections Induced by Klebsiella Pneumoniae New Delhi Strain, Acinetobacter Baumannii and Enterococcus Species

Miskiewicz

Ceranowicz

Szymczak

et al. 2018

IJMS

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This review deals with various microbiological activities of ionic liquids, which constitute the first anti-infective defense against multi-drug-resistant bacteria—with a particular emphasis placed on medicine and pharmacology. The quoted data on the biological activity of ionic liquids including their antimicrobial properties (depending on the type of a cation or an anion) and are discussed in view of possible applications in nosocomial infections. Dedicated attention is given to finding infections with the Klebsiella pneumoniae New Delhi strain, Acinetobacter baumannii, and Enterococcus species, which are responsible for the induction of antibiotic resistance in intensive care units. Diagnosis and treatment using current antibiotics is a significant problem in hospital care, and the relevant burden on the health systems of the European Union member states induces the search for new, effective methods of treatment. Ionic liquids, due to their antibacterial effect, can be considered topical and general medications and may provide the basis for treatment to eliminate the antibiotic resistance phenomenon in the future. At present, the number of infections with resistant pathogens in hospitals and outpatient clinics in the European Union is growing. In 2015–2017, a significant incidence of respiratory and bloodstream infections with bacteria resistant to antibiotics from the 3rd generation group of cephalosporins, glycopeptides, and carbapenems were observed. The paper presents examples of synthesized bifunctional salts with at least one pharmaceutically active ion in obtaining a controlled release, controlled delivery, and biological impact on the pathogenic bacteria, viruses and fungi. The ionic liquids obtained in the presented way may find applications in the treatment of wounds and infections.

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Distribution, genetic diversity and antimicrobial resistance of clinically important bacteria from the environment of a tertiary hospital in Malaysia

Cited by 21 publications

References 34 publications

Molecular characterization of clinical and environmental Pseudomonas aeruginosa isolated in a burn center

Molecular characterization of clinical and environmental Pseudomonas aeruginosa isolated in a burn center

Data on whole-genome sequencing of extended-spectrum beta-lactamases producing Enterobacteriaceae isolates from Malaysia

The Use of Liquids Ionic Fluids as Pharmaceutically Active Substances Helpful in Combating Nosocomial Infections Induced by Klebsiella Pneumoniae New Delhi Strain, Acinetobacter Baumannii and Enterococcus Species

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