Distribution and Variation of Bacterial Endosymbiont and “Candidatus Liberibacter asiaticus” Titer in the Huanglongbing Insect Vector, Diaphorina citri Kuwayama

Hosseinzadeh, Saeed; Shams-Bakhsh, Masoud; Mann, Marina; Fattah-Hosseini, Somayeh; Bagheri, Abdoolnabi; Mehrabadi, Mohammad; Heck, Michelle

doi:10.1007/s00248-018-1290-1

Cited by 49 publications

(49 citation statements)

References 60 publications

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“…Our study specifically considered the microbiota composition in females partly because it has been reported that "Candidatus Profftella" and "Candidatus Carsonella" show an increase in the female ovaries in the presence of Las and thus a potentially greater effect on the reproductive ability of D. citri. Also, higher densities of "Candidatus Profftella" and "Candidatus Carsonella" in the bacteriome of Las-infected females as compared to Las-infected males have been reported [10]. This could account for the higher density of the two endosymbionts seen in the current study.…”

Section: Plos Onesupporting

confidence: 46%

“…identified "Candidatus Profftella", "Candidatus Carsonella" and Wolbachia as the endosymbionts with the highest composition [10,[34][35][36].…”

Section: Plos Onementioning

confidence: 99%

“…This has been shown to be effective in mosquitoes where Wolbachia-infected Aedes aegypti produce smaller volumes of saliva [39]. Furthermore, as Wolbachia has been shown to display an increased density in Las-exposed males, the possibility of vector manipulation using Wolbachia that may affect the ability of D. citri to transmit Las has been hypothesized [10].…”

Section: Plos Onementioning

confidence: 99%

“…The presence of the key endosymbionts and their locations within the insect tissue gives insight into their probable effects on the acquisition and transmission of Las by D. citri for example, "Candidatus Profftella" and "Candidatus Carsonella" which are vertically transferred from mother to offspring, have been shown to be localized in the bacteriome, with "Candidatus Carsonella" localizing to the outer layer in the cytoplasm of the bacteriocytes, while "Candidatus Profftella" localizes to the inner syncytial cytoplasm while Wolbachia pipientis wDi is localized the outside layer of the bacteriome [10]. Furthermore, Wolbachia pipientis is present in the Malpighian tubules and within midgut epithelial cells, the outer membrane cells of the scrotal capsule, testes, and accessory glands [10]. Bacterial symbionts have been considered as a potential tool to manipulate reproduction of D. citri or suppress transmission of Las to citrus plants [11].…”

Section: Introductionmentioning

confidence: 99%

“…Bacterial symbionts have been considered as a potential tool to manipulate reproduction of D. citri or suppress transmission of Las to citrus plants [11]. Studies on the endosymbiont densities in different tissues and Las in healthy and Las-exposed, male and female D. citri individuals showed variation among the bacterial densities, for example, and Las is known to have different effects on male and female D. citri endosymbionts [10]. Key endosymbionts of D. citri such as Wolbachia pipientis wDi, "Candidatus Profftella" and "Candidatus Carsonella" have diverse roles within the insect.…”

Section: Introductionmentioning

confidence: 99%

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Microbiome diversity in Diaphorina citri populations from Kenya and Tanzania shows links to China

et al. 2020

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The Asian citrus psyllid (Diaphorina citri) is a key pest of Citrus spp. worldwide, as it acts as a vector for "Candidatus Liberibacter asiaticus (Las)", the bacterial pathogen associated with the destructive Huanglongbing (HLB) disease. Recent detection of D. citri in Africa and reports of Las-associated HLB in Ethiopia suggest that the citrus industry on the continent is under imminent threat. Endosymbionts and gut bacteria play key roles in the biology of arthropods, especially with regards to vector-pathogen interactions and resistance to antibiotics. Thus, we aim to profile the bacterial genera and to identify antibiotic resistance genes within the microbiome of different populations worldwide of D. citri. The metagenome of D. citri was sequenced using the Oxford Nanopore full-length 16S metagenomics protocol, and the "What's in my pot" (WIMP) analysis pipeline. Microbial diversity within and between D. citri populations was assessed, and antibiotic resistance genes were identified using the WIMP-ARMA workflow. The most abundant genera were key endosymbionts of D. citri ("Candidatus Carsonella", "Candidatus Profftella", and Wolbachia). The Shannon diversity index showed that D. citri from Tanzania had the highest diversity of bacterial genera (1.92), and D. citri from China had the lowest (1.34). The Bray-Curtis dissimilarity showed that China and Kenya represented the most diverged populations, while the populations from Kenya and Tanzania were the least diverged. The WIMP-ARMA analyses generated 48 CARD genes from 13 bacterial species in each of the populations. Spectinomycin resistance genes were the most frequently found, with an average of 65.98% in all the populations. These findings add to the knowledge on the diversity of the African D. citri populations and the probable introduction source of the psyllid in these African countries.

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Section: Plos Onesupporting

confidence: 46%

“…identified "Candidatus Profftella", "Candidatus Carsonella" and Wolbachia as the endosymbionts with the highest composition [10,[34][35][36].…”

Section: Plos Onementioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Microbiome diversity in Diaphorina citri populations from Kenya and Tanzania shows links to China

et al. 2020

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Wolbachia promotes successful sex with siblings in the parasitoid Habrobracon hebetor

Bagheri

Talebi

Asgari

et al. 2021

Pest Management Science

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BACKGROUND Wolbachia are intracellular α‐proteobacteria that have a wide distribution among various arthropods and nematodes. They affect the host reproduction favoring their maternal transmission, which sets up a potential conflict in inbreeding situations when the host avoids sexual reproduction preventing inbreeding depression, while Wolbachia pushes it. We used the wasp Habrobracon hebetor to test the hypothesis that Wolbachia modulates inbreeding avoidance behavior and promotes sib mating. RESULTS Our results showed no obvious pre‐copulatory inbreeding avoidance in this wasp. However, H. hebetor showed a strong post‐copulatory inbreeding avoidance behavior that resulted in a low fertilization rate of uninfected siblings and therefore high rate of production of male progeny was obtained. We observed higher rates of fertilization success in the Wolbachia‐infected lines that resulted in significantly higher female progeny production compared to the uninfected sib mates. Since diploid females are the result of successful fertilization due to haplodiploidy sex determination system in this insect, our results indicate that Wolbachia promoted fertile sib mating in H. hebetor. Interestingly, the rate of adult emergence in the progeny of Wolbachia‐infected sib mates were almost similar to the non‐sib mate crosses and significantly more than those observed in the uninfected sib mate crosses. CONCLUSION Our results support the idea that Wolbachia modulates inbreeding avoidance and promotes sib mating and also mitigates inbreeding depression. By promoting successful sex with siblings and increasing the probability of female progeny, Wolbachia enhances its transmission to the next generation. This is an undescribed effect of Wolbachia on the host reproduction. © 2021 Society of Chemical Industry.

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Insect Pathogenic Viruses, Microsporidians and Endosymbionts

Mehrabadi

Darsouei

Karimi

2021

Progress in Biological Control

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Distribution and Variation of Bacterial Endosymbiont and “Candidatus Liberibacter asiaticus” Titer in the Huanglongbing Insect Vector, Diaphorina citri Kuwayama

Cited by 49 publications

References 60 publications

Microbiome diversity in Diaphorina citri populations from Kenya and Tanzania shows links to China

Microbiome diversity in Diaphorina citri populations from Kenya and Tanzania shows links to China

Wolbachia promotes successful sex with siblings in the parasitoid Habrobracon hebetor

Insect Pathogenic Viruses, Microsporidians and Endosymbionts

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