Distribution and typology of glucose oxidase activity in the genus Penicillium

Abstract

Glucose oxidase (GOD) production by 84 strains of the genus Penicillium was studied in order to define the distribution of the activity among and within the species. Plate screening and sub‐screening in shake culture showed a rather homogeneous behaviour of strains belonging to the same species. Penicillium chrysogenum, P. expansum, P. italicum and P. variabile had a concomitant good production of GOD and catalase activity; peroxidase and polyphenol oxidase were never present. The capability of the culture filtrates to oxidase substrates other than glucose was also studied.

Glucose oxidase, catalase and gluconic acid production by immobilized mycelium of Penicillum variabile P16

“…GO activity was measured spectrophotometrically (Petruccioli et al, 1993) following the enzymatic reduction of benzoquinone to hydroquinone at 290 nm (e=2.31 mM "1 cm'l). CAT was quantified spectrophotometrically, as previously described (Petruccioli et al, 1993), by following the decrease in the absorbance at 240 nm caused by the enzymatic decomposition of hydrogen peroxide (e--43.6 mM -1 cm-1). Gluconic acid content of the culture broths was measured by the D-gluconic acid/D-glucono-8-1actone test-combination kit (Boehringer, Mannheim).…”

“…Fungal GOs have been widely studied during the past two decades (Crueger and Crueger, 1990) and there is much literature on the enzyme and cell immobiliT~tion for the production of gluconic acid (Karube et al, 1977;Doppner and Hartmeier, 1984); on the contrary, only one paper has been found concerning the production of GO from immobilized growing fungal cells (Fiedurek and Ilczuk, 1991). This paper deals with the production of a new and specific GO by a selected strain of Penicillium variabile (Petruccioli et al, 1993;Garzillo et aL, 1994) immobilized in different carriers (Ca-alginate, agar, polyurethane sponge, pearlite and active carbon) and used in repeated-batch processes. Catalase activity (CAT) and gluconic acid production during GO fermentation were also investigated.…”

Synthesis of glucose oxidase and catalase by Aspergillus niger in resting cell culture system

“…Recently, several papers have described the optimal culture conditions for the production of GOD ( Petruccioli et al . 1993 ; Hatzinikolaou & Macris 1995; Hatzinikolaou et al .…”

UTILIZATION OF RAM HORN PEPTONE IN THE PRODUCTION OF GLUCOSE OXIDASE BY A LOCAL ISOLATEAspergillus nigerOC-3

“…[25] The reaction mixture consisted of 2 mL D-glucose (1 M) in sodium citrate buffer (0.1 M, pH 5.0), 1 mL benzoquinone (0.1%, w/v), and 100 ml enzyme source. The equation for calculation of activity is:…”

“…Analytical Methods GO production was determined by measuring extracellular GO activity in the supernatants spectrophotometrically using the method of Petruccioli et al (1993). [25] The reaction mixture consisted of 2 mL D-glucose (1 M) in sodium citrate buffer (0.1 M, pH 5.0), 1 mL benzoquinone (0.1%, w/v), and 100 ml enzyme source.…”