Distribution and Transfer of Antibiotic Resistance Genes in Coastal Aquatic Ecosystems of Bohai Bay

Jia, Lei; Líu, Hao; Zhao, Naiqin; Deng, Qiuxia; Zhu, Chunhua; Zhang, Bo

doi:10.3390/w14060938

Cited by 4 publications

(2 citation statements)

References 46 publications

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“…Indeed, studies have observed that the level of resistance genes, e.g. the resistome, is increased in animals treated with antibiotics [ 22 , 37 ], and also that early antibiotic treatment can have lasting effects on the resistome in weaned pigs [ 17 ]. The most commonly observed resistance genes in European pigs, including Denmark, are genes encoding resistance towards tetracycline, macrolides, β-lactams and aminoglycosides [ 37 ].…”

Section: Introductionmentioning

confidence: 99%

The effects of antibiotic use on the dynamics of the microbiome and resistome in pigs

Tams,

Larsen,

Hansen

et al. 2023

anim microbiome

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Antibiotics are widely used in pig farming across the world which has led to concerns about the potential impact on human health through the selection of antibiotic resistant pathogenic bacteria. This worry has resulted in the development of a production scheme known as pigs Raised Without Antibiotics (RWA), in which pigs are produced in commercial farms, but are ear-tagged as RWA until slaughter unless they receive treatment, thus allowing the farmer to sell the pigs either as premium priced RWA or as conventional meat. Development of antibiotic resistance in pig farming has been studied in national surveys of antibiotic usage and resistance, as well as in experimental studies of groups of pigs, but not in individual pigs followed longitudinally in a commercial pig farm. In this study, a cohort of RWA designated pigs were sampled at 10 time points from birth until slaughter along with pen-mates treated with antibiotics at the same farm. From these samples, the microbiome, determined using 16S sequencing, and the resistome, as determined using qPCR for 82 resistance genes, was investigated, allowing us to examine the difference between RWA pigs and antibiotic treated pigs. We furthermore included 176 additional pigs from six different RWA farms which were sampled at the slaughterhouse as an endpoint to substantiate the cohort as well as for evaluation of intra-farm variability. The results showed a clear effect of age in both the microbiome and resistome composition from early life up until slaughter. As a function of antibiotic treatment, however, we observed a small but significant divergence between treated and untreated animals in their microbiome composition immediately following treatment, which disappeared before 8 weeks of age. The effect on the resistome was evident and an effect of treatment could still be detected at week 8. In animals sampled at the slaughterhouse, we observed no difference in the microbiome or the resistome as a result of treatment status but did see a strong effect of farm origin. Network analysis of co-occurrence of microbiome and resistome data suggested that some resistance genes may be transferred through mobile genetic elements, so we used Hi-C metagenomics on a subset of samples to investigate this. We conclude that antibiotic treatment has a differential effect on the microbiome vs. the resistome and that although resistance gene load is increased by antibiotic treatment load, this effect disappears before slaughter. More studies are needed to elucidate the optimal way to rear pigs without antibiotics.

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Section: Introductionmentioning

confidence: 99%

The effects of antibiotic use on the dynamics of the microbiome and resistome in pigs

Tams,

Larsen,

Hansen

et al. 2023

anim microbiome

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show abstract

“…The ARB, with their resistance genes, can be transferred into adjoining water bodies through erosion and runoff. ARGs in aquatic ecosystems and agricultural waters have been shown to be more easily picked up by bacteria in aquatic environments than those found in the soil [ 151 ]. Mobile genetic elements and plasmids harbouring ARGs have also been found in aquaculture and other aquatic environments.…”

Section: Antibiotic Resistance Genes and One Healthmentioning

confidence: 99%

Review of antibiotic-resistant bacteria and antibiotic resistance genes within the one health framework

Ajayi,

Odeyemi,

Akinjogunla

et al. 2024

Infection Ecology & Epidemiology

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Background: The interdisciplinary One Health (OH) approach recognizes that human, animal, and environmental health are all interconnected. Its ultimate goal is to promote optimal health for all through the exploration of these relationships. Antibiotic resistance (AR) is a public health challenge that has been primarily addressed within the context of human health and clinical settings. However, it has become increasingly evident that antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) that confer resistance are transmitted and circulated within humans, animals, and the environment. Therefore, to effectively address this issue, antibiotic resistance must also be considered an environmental and livestock/wildlife problem. Objective: This review was carried out to provide a broad overview of the existence of ARB and ARGs in One Health settings. Methods: Relevant studies that placed emphasis on ARB and ARGs were reviewed and key findings were accessed that illustrate the importance of One Health as a measure to tackle growing public and environmental threats. Results: In this review, we delve into the complex interplay of the three components of OH in relation to ARB and ARGs. Antibiotics used in animal husbandry and plants to promote growth, treat, and prevent infectious diseases lead to the development of antibiotic-resistant bacteria in animals. These bacteria are transmitted from animals to humans through food and environmental exposure. The environment plays a critical role in the circulation and persistence of antibiotic-resistant bacteria and genes, posing a significant threat to human and animal health. This article also highlights how ARGs are spread in the environment through the transfer of genetic material between bacteria. This transfer can occur naturally or through human activities such as the use of antibiotics in agriculture and waste management practices. Conclusion: It is important to integrate the One Health approach into the public health system to effectively tackle the emergence and spread of ARB and genes that code for resistance to different antibiotics.

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Urbanization influences the indoor transfer of airborne antibiotic resistance genes, which has a seasonally dependent pattern

Zhao,

Liu,

Tan

et al. 2024

Environment International

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Distribution and Transfer of Antibiotic Resistance Genes in Coastal Aquatic Ecosystems of Bohai Bay

Cited by 4 publications

References 46 publications

The effects of antibiotic use on the dynamics of the microbiome and resistome in pigs

The effects of antibiotic use on the dynamics of the microbiome and resistome in pigs

Review of antibiotic-resistant bacteria and antibiotic resistance genes within the one health framework

Urbanization influences the indoor transfer of airborne antibiotic resistance genes, which has a seasonally dependent pattern

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