Distribution and relationship between antimicrobial resistance genes and heavy metals in surface sediments of Taihu Lake, China

Ohore, Okugbe Ebiotubo; Addo, Felix Gyawu; Zhang, Songhe; Han, Nini; Anim-Larbi, Kwaku

doi:10.1016/j.jes.2018.09.004

Cited by 62 publications

(12 citation statements)

References 77 publications

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“…sul3 -integrons are widely distributed within commensal and pathogenic Enterobacteriaceae from humans [ 54 ], a range of food animals including cattle [ 55 ], swine [ 8 ] and poultry [ 56 ] and white stork [ 46 ]. The sul3 gene was reported not only from specimens collected in animal farms, but also from surface sediment in lakes where Cu and Zn resistance genes [ 57 ] were present, and wetlands constructed to treat pig manure [ 58 ]. Notably, sul3 has been detected in tap water, highlighting its importance as an emerging environmental pollutant [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

Genomic Characterisation of a Multiple Drug Resistant IncHI2 ST4 Plasmid in Escherichia coli ST744 in Australia

et al. 2020

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Antibiotic resistance genes (ARGs) including those from the blaCTX-M family and mcr-1 that encode resistance to extended spectrum β–lactams and colistin, respectively, have been linked with IncHI2 plasmids isolated from swine production facilities globally but not in IncHI2 plasmids from Australia. Here we describe the first complete sequence of a multiple drug resistance Australian IncHI2-ST4 plasmid, pTZ41_1P, from a commensal E. coli from a healthy piglet. pTZ41_1P carries genes conferring resistance to heavy-metals (copper, silver, tellurium and arsenic), β-lactams, aminoglycosides and sulphonamides. The ARGs reside within a complex resistance locus (CRL) that shows considerable sequence identity to a CRL in pSDE_SvHI2, an IncHI2:ST3 plasmid from an enterotoxigenic E. coli with serotype O157:H19 of porcine origin that caused substantial losses to swine production operations in Australia in 2007. pTZ41_1P is closely related to IncHI2 plasmids found in E. coli and Salmonella enterica from porcine, avian and human sources in Europe and China but it does not carry genes encoding resistance to clinically-important antibiotics. We identified regions of IncHI2 plasmids that contribute to the genetic plasticity of this group of plasmids and highlight how they may readily acquire new resistance gene cargo. Genomic surveillance should be improved to monitor IncHI2 plasmids.

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Section: Discussionmentioning

confidence: 99%

Genomic Characterisation of a Multiple Drug Resistant IncHI2 ST4 Plasmid in Escherichia coli ST744 in Australia

et al. 2020

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“…And in our study, the stability test showed that the transferred sul3 wild plasmid could inheritance in bacteria for a long time, it also indirectly re ects that sul3 has the potential for long-term transmission. In addition, studies have shown that [27,28], sul3, tetQ, tetO and other sulfonamides and tetracycline resistance genes are signi cantly positively correlated with the content of Cu, Zn and other heavy metals (P < 0.05 or P < 0.01, r = 0.882-0.992), which indicates that heavy metals can help sul3 appear and there may be some heavy metal pollution in the Nanning's farms.…”

Section: Discussionmentioning

confidence: 98%

Antimicrobial and Transconjugants Characteristics of Sul3 Positive Escherichia Coli Isolated from Animals in Nanning, Guangxi Province

Chen

Zhang

et al. 2020

Preprint

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Background: Sulfonamides is the second most popular antibiotic in many countries, which leads to the widespread emergence of sulfonamides resistance. Sul3 is a late sulfanilamide resistance gene, whose research is relatively little. Result: 46 sul3 positive E. coli strains were separated. A total of 12 ST types were observed, and 1 of those was previously unknown type. The ST350 is the most numerous type. All isolates were multidrug-resistant E. coli, with high antimicrobial rates to penicillin, ceftriaxone sodium, streptomycin, tetracycline, ciprofloxacin, gatifloxacin and chloramphenicol (100%, 73.9%, 82.6%, 100%, 80.4%, 71.7% and 97.8%), and with at least 3 resistance genes in addition to sul3. The plasmids transfered from 3 sul3-positive isolates to C600, the most of which brought 7 antibiotic resistance and increased resistance genes to C600. The transferred sul3 gene and the plasmid that carries it could be stably inherited in the recipient bacteria for at least 20 days. Those plasmids had no effect on the growth of the recipient bacteria, but it would greatly reduce (at least 60 time) the in vitro competitiveness of the strains. Conclusions: In Nanning, these sul3-positive Escherichia coli have strong antimicrobial resistance, and the plasmid carrying sul3 has the ability to transfer multiple resistance genes, so long-term monitoring is necessary. Since the transferred plasmid will greatly reduce the in vitro competitiveness of the strain, we can consider limiting the spread of antimicrobial in this respect.

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“…Furthermore, it has been postulated that human multidrug use aggravates co-selective pressure for antibiotic resistance (Xu et al 2016). The occurrence of ARGs also correlates to heavy metals, implying that ARG proliferation is not only dependent on the presence of antibiotics, but also on the presence of heavy metals (Ohore et al 2019).…”

Section: Correlation Between Args and Antibioticsmentioning

confidence: 99%

Monitoring the exposure and emissions of antibiotic resistance: Co-occurrence of antibiotics and resistance genes in wastewater treatment plants

Steenbeek

Timmers

Linde

et al. 2022

Journal of Water and Health

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The COVID-19 pandemic has brought new momentum to ‘wastewater-based epidemiology’ (WBE). This approach can be applied to monitor the levels of antibiotic-resistant genes (ARGs), which in terms are used to make inferences about the burden of antimicrobial resistance (AMR) in human settlements. However, there is still little information about temporal variability in ARG levels measured in wastewater streams and how these influence the inferences made about the occurrence of AMR in communities. The goal of this study was hence to gain insights into the variability in ARG levels measured in the influent and effluent of two wastewater treatment plants in The Netherlands and link these to levels of antibiotic residues measured in the same samples. Eleven antibiotics were detected, together with all selected ARGs, except for VanB. Among the measured antibiotics, significant positive correlations (p>0.70) with the corresponding resistance genes and some non-corresponding ARGs were found. Mass loads varied up to a factor of 35 between days and in concomitance with rainfall. Adequate sampling schemes need to be designed to ensure that conclusions are drawn from valid and representative data. Additionally, we advocate for the use of mass loads to interpret levels of AMR measured in wastewater.

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Distribution and relationship between antimicrobial resistance genes and heavy metals in surface sediments of Taihu Lake, China

Cited by 62 publications

References 77 publications

Genomic Characterisation of a Multiple Drug Resistant IncHI2 ST4 Plasmid in Escherichia coli ST744 in Australia

Genomic Characterisation of a Multiple Drug Resistant IncHI2 ST4 Plasmid in Escherichia coli ST744 in Australia

Antimicrobial and Transconjugants Characteristics of Sul3 Positive Escherichia Coli Isolated from Animals in Nanning, Guangxi Province

Monitoring the exposure and emissions of antibiotic resistance: Co-occurrence of antibiotics and resistance genes in wastewater treatment plants

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