Distribution and quantitative analysis of<i>CIDEa</i>and<i>CIDEc</i>in broiler chickens: accounting for differential fat deposition between strains

Huang, Jingjing; Huang, Liumei; Zeng, Qiaoying; Huang, E. F.; Liang, Haiping; Wei, Qing; Xie, Xianhua; Ruan, Jiming

doi:10.1080/00071668.2017.1415426

Cited by 4 publications

(3 citation statements)

References 31 publications

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“…This result was consistent with the fact that in ammatory cell in ltration has been documented to commonly occur in adipose tissue and stimulate the activation of the immune defense system [50]. In addition, we also found that many pathways related to lipid metabolism (Cholesterol metabolism and Arachidonic acid metabolism) were signi cantly enriched for many DEMs, such as ADIPOQ, CIDEC, SOCS2, SCD and ACAA2, that have been reported to participate in lipid metabolism [51][52][53][54][55]. Many DEMs, such as C1QC, AGPAT4, SNCA and CAV3, were found to be related to lipid deposition for the rst time [56][57][58].…”

Section: Discussionsupporting

confidence: 89%

Identification and co-expression analysis of long noncoding RNAs and mRNAs involved in the deposition of intramuscular fat in Aohan fine-wool sheep

Han

Liu

et al. 2020

Preprint

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Background: Intramuscular fat (IMF) content has become one of the most important indicators for measuring meat quality, and levels of IMF are affected by various genes. Long non-coding RNAs (lncRNAs) are widely expressed non-coding RNAs that play an important regulatory role in a variety of biological processes; however, research on the lncRNAs involved in sheep IMF deposition is still in its infancy. Aohan fine-wool sheep (AFWS), one of China's most important meat-hair, dual-purpose sheep breed, provides a great model for studying the role of lncRNAs in the regulation of IMF deposition. We identified lncRNAs by RNA sequencing in longissimus dorsi muscle (LDM) samples of sheep at two ages: 2 months (Mth-2) and 12 months (Mth-12). Results: We identified a total of 26,247 genes and 6,935 novel lncRNAs in LDM samples of sheep. Among these, 606 mRNAs and 408 lncRNAs were differentially expressed. We then compared the structural characteristics of lncRNAs and mRNAs. We obtained target genes of differentially expressed lncRNAs (DELs) and performed enrichment analyses using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG). We found that target mRNAs were primarily enriched in lipid metabolism, lipid transport, regulation of primary metabolic processes and developmental pathways. Based on the results of important KEGG pathways, we obtained six candidate lncRNAs that potentially regulate lipid deposition and constructed an lncRNA-mRNA co-expression network that included MSTRG.792.1- SCD , MSTRG.8227.1- ACAA2 , MSTRG.10679.1- FADS2 , MSTRG.21942.1- PLA2G4E , MSTRG.21380.1- FZD4 and MSTRG.9270.1- ULK1 . We speculated that these candidate lncRNAs might play a role by regulating the expression of target genes. We randomly selected five mRNAs and five lncRNAs to verify the accuracy of the sequencing data by qRT-PCR. Conclusions: Our study provided a list of the lncRNAs and mRNAs related to intramuscular lipid deposition in sheep and laid a foundation for future research on regulatory mechanisms.

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Section: Discussionsupporting

confidence: 89%

Identification and co-expression analysis of long noncoding RNAs and mRNAs involved in the deposition of intramuscular fat in Aohan fine-wool sheep

Han

Liu

et al. 2020

Preprint

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“…Cell death‐inducing DFF45‐like effector C (CIDEC) may be a key molecule in regulating renal apoptosis and autophagy deficiency induced by insulin resistance. Studie has shown the importance of CIDEC in regulating apoptosis 11 , 12 , 13 , 14 . Under the condition of high glucose, early growth response factor (EGR)1 can induce DN by enhancing extracellular matrix to deposit and mesangial cells to proliferate, partly due to TGF‐β1 upregulation mediated by EGR1 under high glucose conditions 15 .…”

Section: Introductionmentioning

confidence: 99%

CIDEC silencing attenuates diabetic nephropathy via inhibiting apoptosis and promoting autophagy

Zheng

Tan

Shang

et al. 2021

J of Diabetes Invest

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Objective The role of cell death‐inducing DFF45‐like effector C (CIDEC) in insulin resistance has been established, and it is considered to be an important trigger factor for the progression of diabetic nephropathy (DN). We intend to explore whether CIDEC plays an important role in the regulation of DN and its potential mechanism. Methods High‐fat diet and low dose streptozotocin were used to establish type 2 diabetic rat model. We investigate the role of CIDEC in the pathogenesis and process of DN through histopathological analysis, western blot and gene silencing. Meanwhile, the effect of CIDEC on renal tubular epithelial cells stimulated by high glucose was also verified. Results DM group exhibited glucose and lipid metabolic disturbance, with hypertrophy of kidneys, damaged renal function, increased apoptosis, decreased autophagy, glomerulosclerosis and interstitial fibrosis. CIDEC gene silencing improved metabolic disorder and insulin resistance, alleviated renal hypertrophy and renal function damage, decreased glomerular and tubular apoptosis, increased autophagy and inhibited renal fibrosis. At the cellular level, high glucose stimulation increased CIDEC expression in renal tubular epithelial cells, accompanied by increased apoptosis and decreased autophagy. CIDEC gene silencing can improve autophagy and reduce apoptosis. At the molecular level, CIDEC gene silencing also decreased the expression of early growth response factor (EGR)1 and increased the expression of adipose triglyceride lipase (ATGL). Conclusion CIDEC gene silencing may delay the progression of DN by restoring autophagy activity and inhibiting apoptosis with the participation of EGR1and ATGL.

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“…Most studies of Cidea and Cidec in fatty liver focused on mice and human. In poultry, our group's previous study showed that both Cidea and Cidec mRNA transcripts were predominantly but variably expressed in white adipose tissue (WAT), and Cidea and Cidec mRNA levels in white adipose and liver tissues were significantly higher in fat‐type broiler lines than in their lean counterparts (Huang et al., ). Our observations suggest that the functions of Cidea and Cidec reflect inherent characteristics of lipid metabolism that contribute to the differences in fat deposition between strains.…”

Section: Introductionmentioning

confidence: 99%

Effects of a high energy and low protein diet on hepatic and plasma characteristics and Cidea and Cidec mRNA expression in liver and adipose tissue of laying hens with fatty liver hemorrhagic syndrome

Peng

Huang²,

Ruan

et al. 2018

Animal Science Journal

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Cidea and Cidec are two members of Cell death-inducing DNA fragmentation factoralpha-like effector family proteins, which could be involved in lipid or fat metabolism.To better understand the roles of Cidea and Cidec in fatty liver hemorrhagic syndrome (FLHS), 150 healthy 155-day-old Hyline Brown laying hens were randomly divided into control group (fed with basic diet) and experimental group (fed with high-energy low-protein [HELP] diet). Analysis of the liver by tissue sectioning and hematoxylin and eosin staining showed that the HELP diet induced micro-vesicular steatosis in laying hens. Subsequently, based on the liver color scores and the range of lipid accumulation observed in histological examination, we classified livers with <50% vacuolization as mild FLHS and >50% as severe FLHS. The results showed that the levels of Cidea and Cidec mRNA expression were markedly elevated in the liver and adipose tissues with FLHS and the levels of Cidea and Cidec mRNA expression in the liver with severe FLHS were significantly higher than that in the liver with mild FLHS. Thus, the present study revealed that the Cidea and Cidec genes may be involved in pathways of FLHS formation. K E Y W O R D SCidea, Cidec, fat metabolism, fatty liver hemorrhagic syndrome, laying hen

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Distribution and quantitative analysis ofCIDEaandCIDEcin broiler chickens: accounting for differential fat deposition between strains

Cited by 4 publications

References 31 publications

Identification and co-expression analysis of long noncoding RNAs and mRNAs involved in the deposition of intramuscular fat in Aohan fine-wool sheep

Identification and co-expression analysis of long noncoding RNAs and mRNAs involved in the deposition of intramuscular fat in Aohan fine-wool sheep

CIDEC silencing attenuates diabetic nephropathy via inhibiting apoptosis and promoting autophagy

Effects of a high energy and low protein diet on hepatic and plasma characteristics and Cidea and Cidec mRNA expression in liver and adipose tissue of laying hens with fatty liver hemorrhagic syndrome

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