Distribution and properties of cardiac and pulmonary β-adrenergic receptors in corn snakes (Pantherophis guttatus) and Boa constrictor (Boa constrictor)

Abraham, Getu; Hoffmann, Sandra; Hochheim, Ina; Pees, Michael

doi:10.1016/j.cbpa.2019.03.023

Cited by 4 publications

(2 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, the KEGG pathway analysis suggests greater impact of adrenergic stimulation on the right than the left side of the ventricle. The abundant expression of the adrenergic receptor Adrb1 is consistent with a recent pharmacological investigation suggesting the β1-adrenergic receptor is the dominant subtype expressed in snake myocardium ( Abraham et al, 2019 ). Although our data suggest evolutionary conservation of adrenergic signaling, the sensitivity of the ventricular myocardium for subtypes of β-adrenergic receptors may differ across ectotherms ( Skeberdis et al, 1997 ).…”

Section: Discussionsupporting

confidence: 86%

Catecholamines are key modulators of ventricular repolarization patterns in the ball python (Python regius)

Boukens

Joyce

Kristensen

et al. 2021

Journal of General Physiology

View full text Add to dashboard Cite

Ectothermic vertebrates experience daily changes in body temperature, and anecdotal observations suggest these changes affect ventricular repolarization such that the T-wave in the ECG changes polarity. Mammals, in contrast, can maintain stable body temperatures, and their ventricular repolarization is strongly modulated by changes in heart rate and by sympathetic nervous system activity. The aim of this study was to assess the role of body temperature, heart rate, and circulating catecholamines on local repolarization gradients in the ectothermic ball python (Python regius). We recorded body-surface electrocardiograms and performed open-chest high-resolution epicardial mapping while increasing body temperature in five pythons, in all of which there was a change in T-wave polarity. However, the vector of repolarization differed between individuals, and only a subset of leads revealed T-wave polarity change. RNA sequencing revealed regional differences related to adrenergic signaling. In one denervated and Ringer’s solution–perfused heart, heating and elevated heart rates did not induce change in T-wave polarity, whereas noradrenaline did. Accordingly, electrocardiograms in eight awake pythons receiving intra-arterial infusion of the β-adrenergic receptor agonists adrenaline and isoproterenol revealed T-wave inversion in most individuals. Conversely, blocking the β-adrenergic receptors using propranolol prevented T-wave change during heating. Our findings indicate that changes in ventricular repolarization in ball pythons are caused by increased tone of the sympathetic nervous system, not by changes in temperature. Therefore, ventricular repolarization in both pythons and mammals is modulated by evolutionary conserved mechanisms involving catecholaminergic stimulation.

show abstract

Section: Discussionsupporting

confidence: 86%

Catecholamines are key modulators of ventricular repolarization patterns in the ball python (Python regius)

Boukens

Joyce

Kristensen

et al. 2021

Journal of General Physiology

View full text Add to dashboard Cite

show abstract

“…Abramochkin et al, 2022; Aho and Vornanen, 2001; Ask et al, 1980; Gamperl et al, 1994; Gesser et al, 1982; Gilbert et al, 2019; Llach et al, 2004; Lortie and Moon, 2003; Miller et al, 2011; Milligan, 1991; Nickerson et al, 2003; Petersen et al, 2013; Sandblom and Axelsson, 2006; Shiels and Farrell, 1997; Vornanen, 1998; Wood and Shelton, 1975). Amongst the most salient findings, whilst ß1-adrenergic receptors are known to be highly expressed in the hearts other vertebrates, especially tetrapods (Abraham et al, 2019; Rohrer et al, 1996), the rainbow trout heart is understood to express virtually exclusively ß2-ARs (Gamperl et al, 1994). In addition, rainbow trout red blood cells were initially believed to express a ß1-AR (Tetens et al, 1988) but later gene expression and cloning experiments identified it to be a ß3-AR (Nickerson et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Evolutionary loss of the ß1-adrenergic receptor in salmonids

Joyce

2023

Preprint

View full text Add to dashboard Cite

Whole-genome duplications (WGDs) have been at the heart of the diversification of β-adrenergic receptors (β-ARs) in vertebrates. Non-teleost jawed vertebrates typically possess three β-AR genes:adrb1(β1-AR),adrb2(β2-AR), andadrb3(β3-AR), originating from the ancient 2R (two rounds) WGDs. Teleost fishes, owing to the teleost-specific WGD, have five ancestraladrbparalogs (adrb1,adrb2a,adrb2b,adrb3aandadrb3b). Salmonids are particularly intriguing from an evolutionary perspective as they experienced an additional WGD after separating from other teleosts. Moreover, adrenergic regulation in salmonids, especially rainbow trout, has been intensively studied for decades. However, the repertoire ofadrbgenes in salmonids has not been yet characterized. An exhaustive genome survey of diverse salmonids, spanning five genera, complemented by phylogenetic sequence analysis, revealed each species has sevenadrbparalogs: twoadrb2a, twoadrb2b, twoadrb3aand oneadrb3b. Surprisingly, salmonids emerge as the first known jawed vertebrate lineage to lackadrb1.adrb1is nevertheless highly expressed in the hearts of non-salmonid teleosts, indicating that the wealth of data on adrenergic regulation in salmonids should be generalised to other teleost fishes with caution. It is hypothesised that the loss ofadrb1could have been viable because of the evolutionary radiation ofadrb2andadrb3genes attributable to the salmonid WGD.

show abstract

Evolutionary loss of the β1-adrenergic receptor in salmonids

Joyce¹

2023

General and Comparative Endocrinology

View full text Add to dashboard Cite

Distribution and properties of cardiac and pulmonary β-adrenergic receptors in corn snakes (Pantherophis guttatus) and Boa constrictor (Boa constrictor)

Cited by 4 publications

References 44 publications

Catecholamines are key modulators of ventricular repolarization patterns in the ball python (Python regius)

Catecholamines are key modulators of ventricular repolarization patterns in the ball python (Python regius)

Evolutionary loss of the ß1-adrenergic receptor in salmonids

Evolutionary loss of the β1-adrenergic receptor in salmonids

Contact Info

Product

Resources

About