Distribution and Phenotypes of Duodenal Intraepithelial γ/δ T Cells in Patients with Various Types of Primary B-Cell Deficiency

Nilssen, D E; Halstensen, Trond S.; Frøland, Stig S.; Fausa, O; Brandtzæg, Per

doi:10.1006/clin.1993.1131

Cited by 17 publications

(25 citation statements)

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“…Previous studies ofylS cells in primary immunodeficiency have mostly been performed in children and almost exclusively in peripheral blood [17][18][19]. bul we recently found [21] a raised fraction of TCRy/^ ^ duodenal IELs in hypogammaglobulinaemic patients with mild lo moderate inleslinal villous atrophy. We also related the distribution of TCRyM' IELs in IgAD lo the number of eirculaling CD3'.…”

Section: Introductionmentioning

confidence: 64%

“…range 0 3-38':^i) for hisiologically normal conlrols [21]. but IgAD subjects without frequent infections tended to be higher (median 13 6'''i>, rangc2 4 41"/.,) ihan ihc infection-prone ones (median 3 2%) and those in the combined IgAD and IgGSD subgroup (median 4 5''''o) (Fig.…”

Section: Tcryfs In Relation To Cd3 and Cdsmentioning

confidence: 87%

“…Only one specimen showed overt chronic inflammation. In addition to histology, stereomicroscopical and scanning electron-microscopical evaluations were performed to classify villous changes as previously described [21]. This combined evaluation showed normal villi (/i= 13) or partial villous atrophy (H = 4), of which three eases represented borderline changes (see above).…”

Section: Morphological Evaluationmentioning

confidence: 99%

“…Dyspepsia, growth retardation, irritable bowel syndrome, and hypolhyroid disease were ihe main reason.s Ibr endoscopic investigations in these individuals. Also, the numbers of CD3' and TCRylS' IELs per length unit muscularis mucosae were determined for the same material [21]. Referenee data were available in our laboratory for ihe distribution of IgA-.…”

Section: Control Rejerence Datamentioning

confidence: 99%

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Duodenal intraepithelial γ/δ T cells and soluble CD8, neopterin, and β2-microglobulin in serum of IgA-deficient subjects with or without IgG subclass deficiency

Nilssen¹,

Aukrust

et al. 1993

Clinical and Experimental Immunology

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SUMMARYExpression of the ;/^ Tcell receptor (TCR) on CD3' intraepithelial lymphocytes (lELs) was studied by two-eolour immunofluorescence in duodenal tissue sections from healthy (n = 6) or infeetionprone{/) = 7) subjects wilh seleetive IgA deficiency (IgAD), and subjects (« = 4) with combined IgAD and IgG subclass deficiency, TCRy/VJ* IEL proportions in selective IgAD subjects (median 6 .3"'n, range I 0-41'%.) and in those wilh combined deficiency (median 4-5"A>, range l'2-33%) were well within the range (0 3 38'^ii) for hisiologically normal controls (n=II), but ihe healthy IgAD subgroup tended to show raised TCR)7'(>' IEL proportions (median 13-6'M.) compared with the other two subgroups. Also the number of TCRy/Vi^ lELs per intestinal length iinii was relatively high (median I3-9/mm) in ihe healthy IgAD subjects, and significantly raised (/'<0 ()3) compared with controls (median 3-2 'mm). Paired staining revealed that most TCRy/i'J' lELs in bolh seleetive igAD (98%) and combined deficiency (99';..) were CDH . and a large fraction (median ^4"/.! and 63"^, respectively) expressed the V(5l,/J(5l-encoded epitope. The total number of CD3^ IELs (mostly CD8 ') was simitar lo controls. IgAD subject.s. and especially the healthy subgroup, had significanlly increased serum eoncenlraiions of soluble CD8 (P<0 0002), ncoplerin (/'<() ()05), and 2-microglobulin (/'<0007), which was similar to our previous observations in conmion variable immunodeficiency, and probably reflected stimulation of cell-media ted immunity. In addition, the inereased TCRylS^ IELs might reflect a component of compensatory surface proteelion in the healthy IgAD subgroup.

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Section: Introductionmentioning

confidence: 64%

Section: Tcryfs In Relation To Cd3 and Cdsmentioning

confidence: 87%

Section: Morphological Evaluationmentioning

confidence: 99%

Section: Control Rejerence Datamentioning

confidence: 99%

See 2 more Smart Citations

Duodenal intraepithelial γ/δ T cells and soluble CD8, neopterin, and β2-microglobulin in serum of IgA-deficient subjects with or without IgG subclass deficiency

Nilssen¹,

Aukrust

et al. 1993

Clinical and Experimental Immunology

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“…Gamma-delta T cells are often expanded in biopsies from patients with active celiac disease, 46 autoimmune diseases, 47 and primary B-cell immunodeficiency diseases. 55 The source of duodenal intraepithelial lymphocytes in H. pylori gastritis is not known. Lymphoid aggregates were only seen in a minority (14%) of cases and thus an origin of intraepithelial lymphocytes from duodenal mucosa-associated lymphoid tissue (MALT) cannot be proposed with certainty.…”

Section: Discussionmentioning

confidence: 99%

Duodenal intraepithelial lymphocytosis with normal villous architecture: common occurrence in H. pylori gastritis

et al. 2005

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We have observed expansions of intraepithelial lymphocytes in duodenal biopsies from patients with Helicobacter pylori gastritis. This study was undertaken to prospectively evaluate, unselected, paired gastric and duodenal biopsies from 50 patients with H. pylori gastritis and a comparison group of 30 patients with other types of gastritis (10 autoimmune and 20 reactive) to: (1) quantify duodenal intraepithelial lymphocytes, determine their distribution patterns, epithelial location, and phenotype, and (2) correlate the intraepithelial lymphocyte elevations with various features of gastric and duodenal pathology. Intraepithelial lymphocytes were analyzed with antibodies including CD3, CD8, and TIA-1. A stain for H. pylori was performed on all gastric and duodenal biopsies. Duodenal intraepithelial lymphocytes from patients with H. pylori gastritis (using CD3) ranged from 3 to 42 lymphocytes/100 epithelial cells (mean 18.5) compared to 3 to 18 lymphocytes/100 epithelial cells (mean 6.6) in the comparison group. Intraepithelial lymphocyte elevations were seen in 44% of the duodenal biopsies from patients with H. pylori gastritis (using CD3). Significant differences in the intraepithelial lymphocyte counts between patients with H. pylori gastritis and the comparison group were seen for all three T-cell antigens (Po0.001 for CD3 and CD8 and Po0.002 for TIA-1). Duodenal intraepithelial lymphocytes in the H. pylori þ cases had a latent cytotoxic phenotype, H. pylori was not visualized in any of the duodenal biopsies from patients with H. pylori gastritis, and no patient had clinical evidence of celiac disease. Our study highlights frequent duodenal intraepithelial lymphocytosis in individuals with H. pylori gastritis and the lymphocyte distribution patterns (and numbers) overlapped with those described for celiac disease patients. H. pylori gastritis must be considered as a possible explanation for duodenal intraepithelial lymphocytosis with normal villous architecture, especially when lymphocytosis is patchy, intraepithelial lymphocytes display a 'latent' cytotoxic phenotype, and the clinical findings and serologic profile does not fit celiac disease.

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Mucosal Immunity in Immunodeficiency

Brandtzæg¹,

Nilssen²

1994

Symposium in Immunology III

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Distribution and Phenotypes of Duodenal Intraepithelial γ/δ T Cells in Patients with Various Types of Primary B-Cell Deficiency

Cited by 17 publications

References 0 publications

Duodenal intraepithelial γ/δ T cells and soluble CD8, neopterin, and β2-microglobulin in serum of IgA-deficient subjects with or without IgG subclass deficiency

Duodenal intraepithelial γ/δ T cells and soluble CD8, neopterin, and β2-microglobulin in serum of IgA-deficient subjects with or without IgG subclass deficiency

Duodenal intraepithelial lymphocytosis with normal villous architecture: common occurrence in H. pylori gastritis

Mucosal Immunity in Immunodeficiency

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