Distribution and morphometric characteristics of oxytocin‐ and vasopressin‐immunoreactive neurons in the rabbit hypothalamus

Schimchowitsch, Sarah; Moreau, Catherine; Laurent, F.M.; Stoeckel, M. E.

doi:10.1002/cne.902850303

Cited by 27 publications

(25 citation statements)

References 27 publications

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“…This is in agreement with the observations in rats [27]. In contrast, in rabbits [16], guinea pigs [21] and cats [5], AVPand OXY-IR cells are concentrated in the lateral part and in the medial part of the nucleus, respectively. In monkeys [11], AVP-IR cells are distributed more ventrally and medially than OXY-IR cells.…”

Section: Discussionsupporting

confidence: 92%

“…AVP-and OXY-IR cells were scattered in the LHA, and AVP-IR cells were also found in the SCN, as observed in some species [14,16,20]. In the sagittal view, some AVP-and OXY-IR cells and fibers were found in the area between the PVN and the SON.…”

Section: Discussionsupporting

confidence: 51%

“…On the other hand, OXY-IR cells mostly occurred in the dorsal part of the SON. As in cats [5] and rabbits [16], AVP-IR cells were mainly located in the rostral part of the SON while OXY-IR cells in the caudal part.…”

Section: Discussionmentioning

confidence: 61%

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Immunohistochemical Localization of Corticotropin-Releasing Factor, (Arginine8)-Vasopressin and Oxytocin Neurons in the Goat Hypothalamus.

Kikusui

Takeuchi

Mori

1997

The Journal of Veterinary Medical Science

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ABSTRACT. Distribution patterns of corticotropin-releasing factor (CRF), [arginine 8 ]-vasopressin (AVP) and oxytocin (OXY) neurons were examined immunohistochemically in the female goat hypothalamus. The majority of the CRF immunoreactive (-IR) cells were located in the parvocellular part of the paraventricular nucleus (PVN) with smaller population found in the magnocellular part of the PVN. CRF-IR cells were also found in the suprachiasmatic nucleus, the preoptic area and around the fornix in the caudal part of the hypothalamus. AVP-and OXY-IR cells were similarly distributed in the hypothalamus. The majority of AVP-and OXY-IR cells were observed in the magnocellular part of PVN and the supraoptic nucleus. Smaller numbers of AVP-and OXY-IR cells were found in the parvocellular part of the PVN and lateral hypothalamic area. AVP-IR but not OXY-IR cells were located in the suprachiasmatic nucleus. CRF-IR fibers were concentrated in the external palisade zone of the median eminence (ME) with a few fibers found in the internal palisade zone of the ME, whereas AVP-and OXY-IR fibers were concentrated in the internal palisade zone of the ME with a few fibers found in the external zone. These results support the view that not only CRF but also AVP and OXY are released into the hypophysial portal blood and involved in the control of pituitary endocrine function in ruminant species. -KEY WORDS: [arginine 8 ]-vasopressin, corticotropin-releasing factor, oxytocin, paraventricular hypothalamic nucleus, supraoptic nucleus.J. Vet. Med. Sci. 59(8): 621-628, 1997 neuroendocrinological studies to reveal the intra-brain function of three neuropeptides in the manifestation of stress-related behavior. The Shiba goat, which is a Japanese indigenous breed of goat and its closed colony has been established [10], was used in this study because basic information necessary for neurophysiological studies is available [13,25,26,31,32]. MATERIALS AND METHODSSpecimen: Brain samples were obtained from four adult female Shiba goats (Capra hirucus). They were deeply anesthetized with 30 mg/kg sodium pentobarbital following systemic heparinization (5,000 units/head, i.v.), and then perfused bilaterally via the carotid arteries with 3 l of 0.9% NaCl (the first 1 l of saline contained 10 units/ml heparin), followed by 2.5 l of Zamboni's fixative (4% paraformaldehyde, 7% saturated picric acid in 0.1 M phosphate buffer, pH 7.4) [30].Following the perfusion, the head, from which the parietal bone was carefully removed, was put on the stereotaxic instrument for the goat [13]. The method for the stereotaxic approach was described in detail elsewhere [32]. After taking lateral X-ray photographs, reference needles were inserted into the brain vertically until they reached the basis cranii interna with predetermined distances horizontally from the inter-aural (IA) line according to the brain atlas for the Shiba goat [31]. The brain was carefully removed from the cranium and trimmed down to a block with sections parallel to the reference needles. The ...

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 51%

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Immunohistochemical Localization of Corticotropin-Releasing Factor, (Arginine8)-Vasopressin and Oxytocin Neurons in the Goat Hypothalamus.

Kikusui

Takeuchi

Mori

1997

The Journal of Veterinary Medical Science

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“…On the other hand, NPH + and OX + neurons in the monotreme hypothalamus were much more loosely distributed than in therian hypothalamus Kawata and Sano, 1982;Schimchowitsch et al, 1983Schimchowitsch et al, , 1989Iqbal and Jacobson, 1995;Mai et al, 1997;Cheng et al, 2003]. This was particularly apparent in the monotreme supraoptic nuclei and is consistent with the diffuse nature of SOA and SOR as seen in Nissl-stained sections.…”

Section: Distribution and Morphology Of Neurophysin And Oxytocin Immusupporting

confidence: 67%

The Hypothalamic Supraoptic and Paraventricular Nuclei of the Echidna and Platypus

Ashwell

Lajevardi²,

Cheng³

et al. 2006

Brain Behav Evol

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The monotremes are an intriguing group of mammals that have major differences in their reproductive physiology and lactation from therian mammals. Monotreme young hatch from leathery skinned eggs and are nourished by milk secreted onto areolae rather than through nipples. Parturition and lactation are in part controlled through the paraventricular and supraoptic nuclei of the hypothalamus. We have used Nissl staining, enzyme histochemistry, immunohistochemistry for tyrosine hydroxylase, calbindin, oxytocin, neurophysin and non-phosphorylated neurofilament protein, and carbocyanine dye tracing techniques to examine the supraoptic and paraventricular nuclei and the course of the hypothalamo-neurohypophysial tract in two monotremes: the short-beaked echidna (Tachyglossus aculeatus) and the platypus (Ornithorhynchus anatinus). In both monotremes, the supraoptic nucleus consisted of loosely packed neurons, mainly in the retrochiasmatic position. In the echidna, the paraventricular nucleus was quite small, but had similar chemoarchitectural features to therians. In the platypus, the paraventricular nucleus was larger and appeared to be part of a stream of magnocellular neurons extending from the paraventricular nucleus to the retrochiasmatic supraoptic nucleus. Immunohistochemistry for non-phosphorylated neurofilament protein and carbocyanine dye tracing suggested that hypothalamo-neurohypophysial tract neurons in the echidna lie mainly in the retrochiasmatic supraoptic and lateral hypothalamic regions, but most neurophysin and oxytocin immunoreactive neurons in the echidna were found in the paraventricular, lateral hypothalamus and supraoptic nuclei and most oxytocinergic neurons in the platypus were distributed in a band from the paraventricular nucleus to the retrochiasmatic supraoptic nucleus. The small size of the supraoptic nucleus in the two monotremes might reflect functional aspects of monotreme lactation.

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“…The choice of mice and rabbits was based on the differences in entrainment to the LD-cycle and the number of AVP-ir neurons in the SCN between the two species. Whereas mice are exclusively nocturnal, rabbits are only predominantly nocturnal under undisturbed conditions [12][13][14][15], and AVP-ir cells in the mouse SCN seem to outnumber those in the rabbit SCN [4,37].…”

Section: Introductionmentioning

confidence: 99%

Distribution of AVP and Ca2+-dependent PKC-isozymes in the suprachiasmatic nucleus of the mouse and rabbit

Zee

Bult

1995

Brain Research

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Citation for published version (APA): Zee, E. A. V. D., & Bult, A. (1995). Distribution of AVP and Ca2+-dependent PKC-isozymes in the suprachiasmatic nucleus of the mouse and rabbit. Brain Research, 701(1), 99-107. https://doi.org/10.1016/0006-8993(95)00968-1 Copyright Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons).Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum. AbstractThe suprachiasmatic nucleus (SCN) is the circadian pacemaker in mammals and contains a network of argimne-vasopressin-immunoreactive (AVP-ir) neurons. AVP-recipient cells contain the Vla class of receptors linked to phosphoinositol turnover and protein kinase C (PKC). The present study describes the localization of AVP and the four Ca2+-dependent PKC-isoforms in the mouse and rabbit SCN. An estimate of the numerical density of AVP-ir neurons at the rostral, medial, and caudal level of the SCN revealed that the mouse SCN contains more than twice the number of AVP-ir neurons than the rabbit SCN. Neurons immunostained for AVP or PKC dominated in the dorsomedial and ventrolateral aspects of the mouse SCN, while the central area of the SCN revealed only weakly stained neurons. The rabbit SCN was characterized by a more homogeneous distribution of AVP-ir and PKC-ir neurons. PKCa was the most abundantly expressed isozyme in both species, whereas the presence of the other isoforms differed (mouse: PKC a > PKC/31 > > PKC/3 II > PKCT: rabbit: PKC~ > PKC/3II > PKC7 > PKC/3I). Clear PKCy-positive neurons were only observed in the rabbit SCN, while the mouse SCN predominantly contained immunolabeled fiber tracts for this PKC isozyme. Astrocytes immunoreactive for each PKC isoform were frequently encot, ntered in the rabbit SCN, but were absent in mice. Immunofluorescence double labeling showed that numerous AVP-recipient cells in the mouse SCN were immunopositive for PKCo~, and that nearly all AVP-ir neurons express PKCo~ abundantly. These results substantiate the putative role for PKC a in vasopressinergic signal transduction in the SCN. The differential expression in degree and cell lype of the CaZ+-dependent PKC-isoforms in the mouse and rabbit SCN may be related to the differences observed in circadian timekeeping between the two species.

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Distribution and morphometric characteristics of oxytocin‐ and vasopressin‐immunoreactive neurons in the rabbit hypothalamus

Cited by 27 publications

References 27 publications

Immunohistochemical Localization of Corticotropin-Releasing Factor, (Arginine8)-Vasopressin and Oxytocin Neurons in the Goat Hypothalamus.

Immunohistochemical Localization of Corticotropin-Releasing Factor, (Arginine8)-Vasopressin and Oxytocin Neurons in the Goat Hypothalamus.

The Hypothalamic Supraoptic and Paraventricular Nuclei of the Echidna and Platypus

Distribution of AVP and Ca2+-dependent PKC-isozymes in the suprachiasmatic nucleus of the mouse and rabbit

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