Distribution and molecular characterization of genes encoding CTX-M and AmpC β-lactamases in Escherichia coli isolated from an Indian urban aquatic environment

Bajaj, Priyanka; Singh, Nambram Somendro; Kanaujia, Pawan Kumar; Virdi, Jugsharan Singh

doi:10.1016/j.scitotenv.2014.09.084

Cited by 69 publications

(57 citation statements)

References 49 publications

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“…In our study, bla CTX-M gene was present among 88% isolates dominating other ESBL gene i.e. bla TEM , contrary to the findings of Bajaj et al who reported TEM type to be most prevalent among E. coli isolates from river Yamuna [15]. High prevalence of CTX-M has also been reported by Diwan et al and Reinthaler et al in India and Australia [30,31].…”

Section: Discussioncontrasting

confidence: 84%

“…In the present study, 34.58% of bacterial isolates from heavily polluted Delhi stretch of river Yamuna, India were found to be ESBL producers conferring resistance to cephalosporins. This high prevalence of ESBL positive Enterobacteria-ceae was more than previous reports on E. coli and other gram negative bacteria from river Yamuna [14,15]. Moreover, studies from rivers (n = 40) and lakes (n = 18) of Switzerland and rivers (n = 15) of Taiwan reported more or less similar prevalence (36.2% Enterobacteriaceae and 30% E. coli, respectively) of ESBL producing isolates [18,19].…”

Section: Discussionsupporting

confidence: 37%

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Prevalence and Molecular Characterization of ESBL Producing Enterobacteriaceae from Highly Polluted Stretch of River Yamuna, India

Siddiqui¹,

Mondal²,

Siddiqui³

et al. 2018

Microbiology and Biotechnology Letters

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The rapid increase in number and diversity of Extended Spectrum β-Lactamases (ESBLs) producing Enterobacteriaceae in natural aquatic environment is a major health concern worldwide. This study investigates abundance and distribution of ESBL producing multidrug resistant Enterobacteriaceae and molecular characterization of ESBL genes among isolates from highly polluted stretch of river Yamuna, India. Water samples were collected from ten different sites distributed across Delhi stretch of river Yamuna, during 2014-15. A total of 506 non duplicate Enterobacteriaceae isolates were obtained. Phenotypic detection of ESBL production and antibiotic sensitivity for 15 different antibiotics were performed according to CLSI guidelines (Clinical and Laboratory Standard Institute, 2015). A subset of ESBL positive Enterobacteriaceae isolates were identified by 16S rRNA gene and screened for ESBL genes, such as bla CTX-M , bla TEM and bla OXA . Out of 506 non-duplicate bacterial isolates obtained, 175 (34.58%) were positive for ESBL production. Susceptibility pattern for fifteen antibiotics used in this study revealed higher resistance to cefazolin, rifampicin and ampicillin. A high proportion (76.57%) of ESBL positive isolates showed multidrug resistance phenotype, with MAR index of 0.39 at Buddha Vihar and Old Delhi Railway bridge sampling site. Identification and PCR based characterization of ESBL genes revealed the prevalence of bla CTX-M and bla TEM genes to be 88.33% and 61.66%, respectively. Co-occurrence of bla CTX-M and bla TEM genes was detected in 58.33% of the resistant bacteria. The bla OXA gene was not detected in any isolates. This study highlights deteriorating condition of urban aquatic environment due to rising level of ESBL producing Enterobacteriaceae with multidrug resistance phenotype.

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Section: Discussioncontrasting

confidence: 84%

Section: Discussionsupporting

confidence: 37%

Prevalence and Molecular Characterization of ESBL Producing Enterobacteriaceae from Highly Polluted Stretch of River Yamuna, India

Siddiqui¹,

Mondal²,

Siddiqui³

et al. 2018

Microbiology and Biotechnology Letters

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“…2). Most of the CTX-M-positive E. coli isolates had the bla CTX-M-15 gene, which is quite common in India (11,25,26). The bla CMY-2 , bla CMY-6 , bla CMY-42 , bla OXA-1 , bla OXA-9 , and bla SHV-12 ␤-lactamase genes, in addition to the bla CTX-M gene, were detected in the plasmid sequences (Fig.…”

Section: Discussionmentioning

confidence: 95%

“…Bajaj et al (11) reported that bla TEM was the most widespread (100%) ␤-lactamase gene, followed by bla CTX-M (16%), among 61 E. coli isolates which originated from a river in the northern part of India. They did not select E. coli isolates according to their antimicrobial resistance, while we selected E. coli isolates showing resistance to ESC and/or carbapenem for the detailed analyses.…”

Section: Discussionmentioning

confidence: 99%

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Distribution and Relationships of Antimicrobial Resistance Determinants among Extended-Spectrum-Cephalosporin-Resistant or Carbapenem-Resistant Escherichia coli Isolates from Rivers and Sewage Treatment Plants in India

Akiba

Sekizuka

Yamashita

et al. 2016

Antimicrob Agents Chemother

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kTo determine the distribution and relationship of antimicrobial resistance determinants among extended-spectrum-cephalosporin (ESC)-resistant or carbapenem-resistant Escherichia coli isolates from the aquatic environment in India, water samples were collected from rivers or sewage treatment plants in five Indian states. A total of 446 E. coli isolates were randomly obtained. Resistance to ESC and/or carbapenem was observed in 169 (37.9%) E. coli isolates, which were further analyzed. These isolates showed resistance to numerous antimicrobials; more than half of the isolates exhibited resistance to eight or more antimicrobials. The bla NDM gene was detected in 14/21 carbapenem-resistant E. coli isolates: bla NDM-1 in 2 isolates, bla NDM-5 in 7 isolates, and bla NDM-7 in 5 isolates. The bla CTX-M gene was detected in 112 isolates (66.3%): bla CTX-M-15 in 108 isolates and bla CTX-M-55 in 4 isolates. We extracted 49 plasmids from selected isolates, and their whole-genome sequences were determined. Fifty resistance genes were detected, and 11 different combinations of replicon types were observed among the 49 plasmids. The network analysis results suggested that the plasmids sharing replicon types tended to form a community, which is based on the predicted gene similarity among the plasmids. Four communities each containing from 4 to 17 plasmids were observed. Three of the four communities contained plasmids detected in different Indian states, suggesting that the interstate dissemination of ancestor plasmids has already occurred. Comparison of the DNA sequences of the bla NDM -positive plasmids detected in this study with known sequences of related plasmids suggested that various mutation events facilitated the evolution of the plasmids and that plasmids with similar genetic backgrounds have widely disseminated in India.

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Antibiotic Resistance and Sanitation in India: Current Situation and Future Perspectives

Sasikaladevi

Eswari

Nambi

2020

The Handbook of Environmental Chemistry

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Distribution and molecular characterization of genes encoding CTX-M and AmpC β-lactamases in Escherichia coli isolated from an Indian urban aquatic environment

Cited by 69 publications

References 49 publications

Prevalence and Molecular Characterization of ESBL Producing Enterobacteriaceae from Highly Polluted Stretch of River Yamuna, India

Prevalence and Molecular Characterization of ESBL Producing Enterobacteriaceae from Highly Polluted Stretch of River Yamuna, India

Distribution and Relationships of Antimicrobial Resistance Determinants among Extended-Spectrum-Cephalosporin-Resistant or Carbapenem-Resistant Escherichia coli Isolates from Rivers and Sewage Treatment Plants in India

Antibiotic Resistance and Sanitation in India: Current Situation and Future Perspectives

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