Distribution and induction of cytochrome P450 1A1 in the rainbow trout brain

Andersson, Tommy; Goksøyr, Anders

doi:10.1007/bf00003438

Cited by 14 publications

(4 citation statements)

References 30 publications

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“…Furthermore, in the present study, EROD activity in microsome was slightly lower than that in tissues. This result was confirmed by Andersson and Goksyr (1994), who found that EROD activity in the whole‐brain homogenate of rainbow trout in a control and a b‐naphthoflavone exposure group was higher than that of microsome in the whole‐brain tissue. At present, no study detecting GST activity in subcellular fractions of bivalves has been found.…”

Section: Discussionsupporting

confidence: 74%

Bioaccumulation, Detoxification, and Biological Macromolecular Damage of Benzo[a]pyrene in Exposure in Tissues and Subcellular Fractions of Scallop Chlamys farreri

Chen

Miao

et al. 2022

Enviro Toxic and Chemistry

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Because of the persistence and high toxicity of benzo[a]pyrene (B[a]P), the bioaccumulation and detoxification mechanisms of B[a]P have been studied extensively at the tissue level; but the data at the subcellular level in bivalves have not been reported. The present study was conducted to investigate the effects of B[a]P exposure on bioaccumulation, detoxification, and biomacromolecular damage in gills, digestive glands, and their subcellular fractions of the scallop Chlamys farreri. The subcellular fraction contains cytoplasm, mitochondria, microsome, nucleus, cell membrane, and overall organelle. The results demonstrated that B[a]P accumulation showed a clear time–dose effect. Based on the time‐dependent accumulation of B[a]P in subcellular fractions, we speculated that the intracellular migration order of B[a]P was cell membrane, organelle, and nucleus in turn. Considering the difference of B[a]P accumulation may be related to B[a]P metabolism, we have further confirmed that the activities of B[a]P metabolizing enzymes in scallop tissues and subcellular fractions were significantly tempted by B[a]P (p < 0.05), including 7‐ethoxyresorufin O‐deethylase (increased), glutathione‐S‐transferase (GST; decreased), and superoxide dismutase (increased). First, GST was detected in bivalve cytoplasm and microsome. Second, B[a]P exposure also caused biomacromolecules damage. The results demonstrated that mitochondria and microsome were more vulnerable to lipid peroxidation than cell membrane and nucleus. Taken together, the present study fills some of the gaps in our knowledge of the bioaccumulation and detoxification mechanisms of C. farreri exposed to B[a]P in subcellular fractions and deeply explores the transportation and the main metabolic and damage sites of polycyclic aromatic hydrocarbons (PAHs) in cells, which helped us to comprehensively understand the toxic mechanism of PAHs on bivalves. Environ Toxicol Chem 2022;41:2353–2364. © 2022 SETAC

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Section: Discussionsupporting

confidence: 74%

Bioaccumulation, Detoxification, and Biological Macromolecular Damage of Benzo[a]pyrene in Exposure in Tissues and Subcellular Fractions of Scallop Chlamys farreri

Chen

Miao

et al. 2022

Enviro Toxic and Chemistry

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“…Therefore, CYP protein in the olfactory bulbs should be constitutively expressed and also highly inducible in response to any acute xenobiotic exposure. Indeed, the presence of constitutive CYP1A1 in fish olfactory systems has been confirmed (Andersson and Goksøyr, 1994;Monod et al, 1994Monod et al, , 1995Ortiz-Delgado et al, 2002), and CYP1A1 immunoreactivities and enzyme activities are induced in the olfactory organs in fish exposed to xenobiotics (Goksøyr and Förlin, 1992;Smolowitz et al, 1992).…”

Section: Discussionmentioning

confidence: 94%

β-naphthoflavone induction of CYP1A in brain of juvenile lake trout(Salvelinus namaycush Walbaum)

Chung-Davidson

Rees

et al. 2004

Journal of Experimental Biology

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Many environmental pollutants induce expression of the cytochrome P450 (CYP) 1A subfamily of genes. We integrated cellular and molecular biological techniques to examine the effects of β-naphthoflavone (BNF) exposure in lake trout brain CYP1A distribution and dynamics. Over a 32-day time-course, real time quantitative reverse transcription polymerase chain reaction (Q-RT-PCR) results showed that CYP1A mRNA induction in response to BNF exposure occurred rapidly and continued to rise in the BNF-treated lake trout after 4·h, with a peak at or after 2·days. Messenger RNA levels fell after 4·days, and this trend continued after 16·days of exposure. In situ hybridization indicated that CYP1A mRNA was universally elevated in the brain of BNF-exposed fish and was mainly expressed in the endothelia and occasionally in the glial cells. CYP1A immunoreactivity was induced in the olfactory bulb and valvula cerebelli of BNF-treated fish. Other brain areas showed constitutive CYP1A immunoreactivity in both control and BNF-treated fish. Some BNF-treated fish contained multifocal hemorrhages in the brain tissue, and these fish had overall depressed CYP1A immunoreactivity in the brain. The relationship between transcriptional and translational effects of BNF exposure in the brain of juvenile lake trout is discussed.

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“…There is also in vivo evidence of enhanced TA metabolism in brains of offspring born to PCB-exposed dams (59), and there are reports that TCDD treatment decreases brain levels of TRP and 5-OH-TA in rats (60). Given that AhR (61) and ARNT (62) have been detected in rats brains using in situ hybridization techniques and the induction of brain CYP1A1 gene expression by HAHs and PAHs in rats (63), humans (64), and trout (65) has been demonstrated, it is possible that potentially serious physiological and toxicological consequences could occur as a result of a perturbation of TRP metabolism by AhR ligands, particularly in the brain.…”

Section: Discussionmentioning

confidence: 99%

Activation of the Ah Receptor by Tryptophan and Tryptophan Metabolites

et al. 1998

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The aryl hydrocarbon receptor (AhR) is a ligand-dependent transcription factor that mediates many of the biological and toxicological actions of a variety of hydrophobic natural and synthetic chemicals, including the environmental contaminant 2,3,7, 8-tetrachlorodibenzo-p-dioxin (TCDD, dioxin). A variety of indole-containing chemicals, such as indole-3-carbinol, indolo[3, 2-b]carbazole, and UV photoproducts of tryptophan (TRP), have previously been identified as ligands for AhR. Here we have examined the ability of endogenous metabolites of tryptophan (TRP) to bind to and activate AhR in vitro and in cells in culture. Although hydroxylated TRP metabolites were inactive, two metabolites, namely tryptamine (TA) and indole acetic acid (IAA), were shown to be AhR agonists. Not only do TA and IAA bind competitively to AhR, but they also can stimulate AhR transformation and DNA binding and induce expression of an AhR-dependent reporter gene in cells. In addition to being an AhR ligand, TA is also a competitive substrate for cytochrome P4501A1, a well-characterized AhR- and TCDD-inducible gene product. Although these compounds are relatively weak ligands, compared to TCDD, they represent some of the first endogenous hydrophilic AhR agonists identified to date.

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Distribution and induction of cytochrome P450 1A1 in the rainbow trout brain

Cited by 14 publications

References 30 publications

Bioaccumulation, Detoxification, and Biological Macromolecular Damage of Benzo[a]pyrene in Exposure in Tissues and Subcellular Fractions of Scallop Chlamys farreri

Bioaccumulation, Detoxification, and Biological Macromolecular Damage of Benzo[a]pyrene in Exposure in Tissues and Subcellular Fractions of Scallop Chlamys farreri

β-naphthoflavone induction of CYP1A in brain of juvenile lake trout(Salvelinus namaycush Walbaum)

Activation of the Ah Receptor by Tryptophan and Tryptophan Metabolites

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