Distribution and in Vitro Availability of Selenium in Selenium-Containing Storage Protein from Selenium-Enriched Rice Utilizing Optimized Extraction

Abstract

Selenium (Se) distribution in Se-enriched rice and optimization of extraction for Se-containing protein were studied. Se availability in Se-containing protein product was simulated using an in vitro gastrointestinal digestion. The results showed that Se was predominately found as organic Se, whereas inorganic Se comprised only 2.85% of the total Se. The glutelin fraction contained the largest amount of Se, approximately 31.3% of the total Se in the rice gain. Utilizing orthogonal analysis, the optimum extraction conditions were selected at a volume to weight of 20:1, 0.08 M NaOH, an extraction time of 3 h, and at a temperature of 35 degrees C. A Se-containing rice protein product with 83.5% protein and 9.09 microg g(-1) Se was sequestered using the optimal extraction method. This rice protein product with high molecular weight Se-containing protein can readily be digested to low molecular weight peptides and selenomethionine (52.3% of total Se in protein extract).

Steam Flash Explosion Assisted Dissolution of Keratin from Feathers

“…In general, a longer extraction time gave a higher extraction rate. 25 However, in the study, the effect of extraction time became evident at the first hours of extraction, after which the yield of keratin changed not significantly. The effect of extraction temperature was conducted at a constant condition of 0.4% NaOH, 2 h, and a solvent to material ratio of 20:1 ( Figure 1D).…”

Quantification and In Vitro Bioaccessibility of Selenium from Osborne Fractions of Selenium‐Rich Cereal Grains

“…In rice, the small proportion of selenium-containing protein was still bound to the starch matrices (Fang et al 2010). In rice, the small proportion of selenium-containing protein was still bound to the starch matrices (Fang et al 2010).…”

The Correlation Between In Vitro Antioxidant Activity and Immunomodulatory Activity of Enzymatic Hydrolysates from Selenium‐Enriched Rice Protein

“…The preparation of Se‐enriched rice was conducted according to our previous study (Fang and others ). The cultivar of rice ( Oryza sativa L.) used was Wuyunjing 23.…”

“…As one of the leading food crops for more than half of the world's population, rice is an important source of Se, especially for the Chinese, who depend on it as a staple food (Fang and others ). Se‐enriched rice has been widely studied owing to its high value for nutrition and health (Fang and others ; Wang and others ; Giacosa and others ). Hu and others () showed the significantly increased the activity of glutathione peroxidase in mice liver and alter the occurrence chemical‐induced mutation by Se‐enriched rice.…”

“…Our previous study showed that the major Se compound identified in Se‐enriched rice was selenomethionine (SeMet), which represented more than 86% of the total Se extracted (Fang and others ). A subsequent study showed that glutelin, the predominant protein in rice, contained the largest amount of SeMet residues, which proved to be digestible by in vitro gastrointestinal digestion (Fang and others ). To the best of our knowledge, there is no evidence that Se‐containing proteins with high molecular weights could exhibit bioactivities.…”