Distribution and immunohistochemical characteristics of cocaine- and amphetamineregulated transcript-positive nerve elements in the pelvic ganglia of the female pig

Zacharko-Siembida, Anna; Matysek, M.; Szalak, Radosław; Radlińska, A; Obszańska, K; Arciszewski, Marcin B.

doi:10.1515/pjvs-2017-0022

Cited by 2 publications

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References 60 publications

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“…All sacral preganglionic neurons are cholinergic and express ChAT and use acetylcholine as the primary transmitter at peripheral synapses in the pelvic ganglia. However, SOM, CAL, and NK1R are examples of markers that identify subclasses of these neurons in the spinal cord or differentiate their projecting terminals in pelvic ganglia ( Sasek et al, 1984 ; Keast and de Groat, 1989 ; Dhami and Mitchell, 1991 ; Keast, 1991 , 1994 ; Papka and McNeill, 1993 ; Jen et al, 1996 ; Pidsudko, 2014 ; Zacharko-Siembida et al, 2017 ). The specific function of classes of sacral preganglionic neurons identified by SOM, CAL, and NK1R in the current study remains to be determined, but in rat pelvic ganglia, preganglionic terminals containing SOM are more commonly associated with evacuative (bladder and bowel) pathways than pathways projecting to the penile cavernosum ( Keast and de Groat, 1989 ).…”

Section: Discussionmentioning

confidence: 99%

Axonal Injury Induces ATF3 in Specific Populations of Sacral Preganglionic Neurons in Male Rats

2018

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Compared to other neurons of the central nervous system, autonomic preganglionic neurons are unusual because most of their axon lies in the periphery. These axons are vulnerable to injury during surgical procedures, yet in comparison to peripheral neurons and somatic motor neurons, the impact of injury on preganglionic neurons is poorly understood. Here, we have investigated the impact of axotomy on sacral preganglionic neurons, a functionally diverse group of neurons required for micturition, defecation, and sexual function. We have previously observed that after axotomy, the injury-related transcription factor activating transcription factor-3 (ATF3) is upregulated in only half of these neurons (Peddie and Keast, 2011: PMID: 21283532). In the current study, we have investigated if this response is constrained to particular subclasses of preganglionic neurons that have specific functions or signaling properties. Seven days after unilateral pelvic nerve transection, we quantified sacral preganglionic neurons expressing ATF3, many but not all of which co-expressed c-Jun. This response was independent of soma size. Subclasses of sacral preganglionic neurons expressed combinations of somatostatin, calbindin, and neurokinin-1 receptor, each of which showed a similar response to injury. We also found that in contrast to thoracolumbar preganglionic neurons, the heat shock protein-25 (Hsp25) was not detected in naive sacral preganglionic neurons but was upregulated in many of these neurons after axotomy; the majority of these Hsp25 neurons expressed ATF3. Together, these studies reveal the molecular complexity of sacral preganglionic neurons and their responses to injury. The simultaneous upregulation of Hsp25 and ATF3 may indicate a distinct mechanism of regenerative capacity after injury.

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