Distribution and Identification of Endophytic <i>Streptomyces</i> Species from <i>Schima wallichii</i> as Potential Biocontrol Agents against Fungal Plant Pathogens

Passari, Ajit Kumar; Mishra, Vineet Kumar; Gupta, Vijai Kumar; Saikia, Ratul; Singh, Bhim Pratap

doi:10.5604/17331331.1215611

Cited by 22 publications

(5 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Members of this phylum are well known for their ability to produce secondary metabolites with application in the agricultural, pharmaceutical and medical industries ( Himaman et al, 2016 ). Several studies proposed their use as BCAs ( El-Tarabily et al, 2000 ; Kunoh, 2002 ; Cao et al, 2005 ; Prapagdee et al, 2008 ; Mingma et al, 2014 ). They play key roles as plant growth promoters, disease resistance inducers and drought tolerance stimulators ( Himaman et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Plant Microbiome and Its Link to Plant Health: Host Species, Organs and Pseudomonas syringae pv. actinidiae Infection Shaping Bacterial Phyllosphere Communities of Kiwifruit Plants

Purahong

Orrù²,

Donati

et al. 2018

Front. Plant Sci.

View full text Add to dashboard Cite

Pseudomonas syringae pv. actinidiae (Psa) is the causal agent of the bacterial canker, the most devastating disease of kiwifruit vines. Before entering the host tissues, this pathogen has an epiphytic growth phase on kiwifruit flowers and leaves, thus the ecological interactions within epiphytic bacterial community may greatly influence the onset of the infection process. The bacterial community associated to the two most important cultivated kiwifruit species, Actinidia chinensis and Actinidia deliciosa, was described both on flowers and leaves using Illumina massive parallel sequencing of the V3 and V4 variable regions of the 16S rRNA gene. In addition, the effect of plant infection by Psa on the epiphytic bacterial community structure and biodiversity was investigated. Psa infection affected the phyllosphere microbiome structures in both species, however, its impact was more pronounced on A. deliciosa leaves, where a drastic drop in microbial biodiversity was observed. Furthermore, we also showed that Psa was always present in syndemic association with Pseudomonas syringae pv. syringae and Pseudomonas viridiflava, two other kiwifruit pathogens, suggesting the establishment of a pathogenic consortium leading to a higher pathogenesis capacity. Finally, the analyses of the dynamics of bacterial populations provided useful information for the screening and selection of potential biocontrol agents against Psa.

show abstract

Section: Discussionmentioning

confidence: 99%

Plant Microbiome and Its Link to Plant Health: Host Species, Organs and Pseudomonas syringae pv. actinidiae Infection Shaping Bacterial Phyllosphere Communities of Kiwifruit Plants

Purahong

Orrù²,

Donati

et al. 2018

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…Among the 56 endophytic bacteria obtained from P. ostii, Bacillus was the most dominant genus (55.4% of all isolates); this finding is consistent with previous reports on different hosts (El-Deeb et al 2013). In addition to Bacillus sp., other genera, such as Streptomyces from Schima wallichi (Passari et al 2016) and Leifsonia from ginseng roots, have also been reported in medicinal plants (Qiu et al 2007). Endophytic Bacillus spp.…”

Section: Discussionmentioning

confidence: 99%

“…4). Both Streptomyces and Pseudomonas are prolific producers of bioactive compounds, including various antibiotics, antitumor, and plant growth hormones (Wu et al 2011;Passari et al 2016). Therefore, these NRPS genes may be capable of producing unique bioactive compounds.…”

Section: Discussionmentioning

confidence: 99%

Culturable Endophytes Diversity Isolated from Paeonia ostii and the Genetic Basis for Their Bioactivity

Yang

Zhang

Dong

et al. 2018

Polish Journal of Microbiology

View full text Add to dashboard Cite

A b s t r a c tPaeonia ostii is known for its excellent medicinal values as Chinese traditional plant. To date, the diversity of culturable endophytes associated with P. ostii is in its initial phase of exploration. In this study, 56 endophytic bacteria and 51 endophytic fungi were isolated from P. ostii roots in China. Subsequent characterization of 56 bacterial strains by 16S rDNA gene sequence analysis revealed that nine families and 13 different genera were represented. All the fungal strains were classed into six families and 12 genera based on ITS gene sequence. The biosynthetic potential of all the endophytes was further investigated by the detection of putative polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) genes. The PCR screens were successful in targeting thirteen bacterial PKS, five bacterial NRPS, ten fungal PKS and nine fungal NRPS gene fragments. Bioinformatic analysis of these detected endophyte gene fragments facilitated inference of the potential bioactivity of endophyte bioactive products, suggesting that the isolated endophytes are capable of producing a plethora of secondary metabolites. These results suggest that endophytes isolated from P. ostii had abundant population diversity and biosynthetic potential, which further proved that endophytes are valuable reservoirs of novel bioactive compounds. K e y w o r d s: Paeonia ostii, endophytes, diversity, polyketide synthase, nonribosomal peptide synthetase Yang R.-X. et al.

show abstract

“…Additionally, they operate as hosts for several endophytes, which are known to produce a variety of bioactive substances [ 25 – 27 ]. Endophytic microorganisms showed a wide range of biological activity, including anticancer, antibacterial, antifungal, immunomodulatory, antioxidant, and textile dyeing activities [ 28 – 30 ]. Endophytic fungi are a significant part of the plant microbiota that is present throughout the plant tissues without harming the host.…”

Section: Introductionmentioning

confidence: 99%

Exploring the potential of Rhizopus oryzae AUMC14899 as a novel endophytic fungus for the production of l-tyrosine and its biomedical applications

2023

View full text Add to dashboard Cite

Background A significant threat to the public's health is the rise in antimicrobial resistance among numerous nosocomial bacterial infections. This may be a detriment to present initiatives to enhance the health of immune-compromised patients. Consequently, attention has been devoted to exploring new bioactive compounds in the field of drug discovery from endophytes. Therefore, this study is the first on the production of l-tyrosine (LT) as a promising bio-therapeutic agent from endophytic fungi. Results A new endophytic fungal isolate has been identified for the first time as Rhizopus oryzae AUMC14899 from Opuntia ficus-indica (L.) and submitted to GenBank under the accession number MZ025968. Separation of amino acids in the crude extract of this fungal isolate was carried out, giving a higher content of LT, which is then characterized and purified. LT exhibited strong antibacterial and anti-biofilm activities against multidrug-resistant Gram-negative and Gram-positive bacteria. The recorded minimum inhibitory concentration (MIC) values ranged from 6 to 20 µg/ml. In addition, LT caused a strong reduction in biofilm formation and disrupted the preformed biofilm. Moreover, results indicated that LT supported cell viability, evidencing hemocompatibility and no cytotoxicity. Conclusion Our findings suggest that LT has potential as a therapeutic agent due to its potential antibacterial, anti-biofilm, hemocompatibility, and lack of cytotoxic activities, which may also increase the range of therapy options for skin burn infections, leading to the development of a novel fungal-based drug.

show abstract

Distribution and Identification of Endophytic Streptomyces Species from Schima wallichii as Potential Biocontrol Agents against Fungal Plant Pathogens

Cited by 22 publications

References 50 publications

Plant Microbiome and Its Link to Plant Health: Host Species, Organs and Pseudomonas syringae pv. actinidiae Infection Shaping Bacterial Phyllosphere Communities of Kiwifruit Plants

Plant Microbiome and Its Link to Plant Health: Host Species, Organs and Pseudomonas syringae pv. actinidiae Infection Shaping Bacterial Phyllosphere Communities of Kiwifruit Plants

Culturable Endophytes Diversity Isolated from Paeonia ostii and the Genetic Basis for Their Bioactivity

Exploring the potential of Rhizopus oryzae AUMC14899 as a novel endophytic fungus for the production of l-tyrosine and its biomedical applications

Contact Info

Product

Resources

About