Distribution and genetic variation of hymenolepidid cestodes in murid rodents on the Canary Islands (Spain)

Abstract

BackgroundIn the Canary Islands there are no previous data about tapeworms (Cestoda) of rodents. In order to identify the hymenolepidid species present in these hosts, a survey of 1,017 murine (349 Rattus rattus, 13 Rattus norvegicus and 655 Mus musculus domesticus) was carried out in the whole Archipelago. Molecular studies based on nuclear ITS1 and mitochondrial COI loci were performed to confirm the identifications and to analyse the levels of genetic variation and differentiation.ResultsThree species of hymenolepidids were identified: Hymenolepis diminuta, Rodentolepis microstoma and Rodentolepis fraterna. Hymenolepis diminuta (in rats) and R. microstoma (in mice) showed a widespread distribution in the Archipelago, and R. fraterna was the least spread species, appearing only on five of the islands. The hymenolepidids found on Fuerteventura, Lanzarote and La Graciosa were restricted to one area. The COI network of H. diminuta showed that the haplotypes from Lanzarote and Fuerteventura are the most distant with respect to the other islands, but clearly related among them.ConclusionsFounder effects and biotic and abiotic factors could have played important role in the presence/absence of the hymenolepidid species in determined locations. The haplotypes from the eastern islands (Fuerteventura and Lanzarote) seem to have shared an ancestral haplotype very distant from the most frequent one that was found in the rest of the islands. Two colonization events or a single event with subsequent isolation and reduced gene flow between western-central and eastern islands, have taken place in the Archipelago. The three tapeworms detected are zoonotic species, and their presence among rodents from this Archipelago suggests a potential health risk to human via environmental contamination in high risk areas. However, the relatively low prevalence of infestations detected and the focal distribution of some of these species on certain islands reduce the general transmission risk to human.

Hymenolepis microstoma (Cestoda: Hymenolepididae) en ratones caseros (Mus musculus) de Lima, Perú

“…La identidad genética de H. microstoma se determinó por la alineación de la secuencia parcial del gen cox1. La secuencia obtenida en este estudio tuvo 418pb y fue alienada con secuencias de H. microstoma obtenidas del GenBank (JN258051, LC063188 y AB494473) (Nkouawa et al 2016, Foronda et al 2011, Okamoto et al 1997. Las secuencias de H. microstoma obtenidas del GenBank fueron colectados de ratones (M. musculus) de España y Japón.…”

“…Los principales reservorios de estos cestodos zoonóticos son los roedores comensales Rattus rattus, Rattus norvegicus y Mus musculus (Foronda et al 2011). Estos roedores actúan como hospederos definitivos para Hymenolepis diminuta, Hymenolepis nana (=Rodentolepis nana) e Hymenolepis microstoma (Nkouawa et al 2016, Foronda et al 2011, Macnish et al 2003. Estos cestodos tienen una distribución cosmopolita y son capaces de infectar a humanos (Nkouawa et al 2016).…”

Parasite fauna of rodents (Murinae) from El Hierro (Canary Islands, Spain): a multidisciplinary approach

“…The fact that R. microstoma was the most prevalent species (27.2%) in M. m. domesticus from El Hierro could be due to the high number of mice studied (n = 75) from Frontera forest place, where this cestode was preferentially located, opposite to that occur in other Canary islands where R. microstoma is highly spread (Foronda et al 2011). However, there is a clear seasonal influence in these results (KW = 25.9, p<0.01).…”

Parasites in synanthropic rodents in municipality of the Northwest region of the State of Paran, Brazil

“…Research on the presence of parasites in synanthropic rodents has been done in different countries like Korea, Iran, Spain, Switzerland, Mexico and Poland (Reperante et al, 2009;Yi et al, 2010;Kia et al, 2010;Foronda et al, 2011;Paziewska, 2011;Jiménez et al, 2012;Mowlavi et al, 2013). In Brazil, studies have been done in the states of Paraná (PR) and Sergipe (SE) (Araujo et al, 2010;Guimarães et al, 2014).…”