Distribution and Expression of Visfatin-Positive Cells in the Spleen of Lipopolysaccharide-Stimulated Piglets

Xiao, Ke; Qiu, Changwei; Chen, Min; Yang, Zhi; Jing, Wang; Peng, Kaiman; Song, Hui

doi:10.4067/s0717-95022014000400052

Cited by 4 publications

(3 citation statements)

References 23 publications

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“…Then, we further con rmed the expression changes of the three lncRNAs in liver, spleen, jejunum and thymus of the piglets challenged with LPS at different times (0, 1, 2, 4, 8, 12 and 24 h). The previous studies demonstrated LPS challenge can induce sever in ammation in the piglet model, causing liver injury, intestinal damage and histological changes of spleen [43][44][45][46][47]. Although the three lncRNAs displayed different tissue expression patterns in the piglets, they all shown abundant expression in liver, spleen, jejunum and thymus.…”

Section: Discussionmentioning

confidence: 95%

Construction and Analysis for Dysregulated lncRNAs and mRNAs in Lipopolysaccharide-Induced Porcine Peripheral Blood Mononuclear Cells

Zhang

Chen

et al. 2020

Preprint

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Background: Lipopolysaccharide (LPS) in the outer membrane of Gram-negative bacteria induces an intense inflammatory response in pigs. Long noncoding RNAs (lncRNAs) are emerging as key regulators in inflammation and immunity. However, their functions and profiles in LPS-induced inflammation in pigs are largely unknown. In this study, we profiled global lncRNA and mRNA expression changes in porcine peripheral blood mononuclear cells (PBMCs) treated with lipopolysaccharide (LPS) using lncRNA-seq technique. Result: Totally 43 differentially expressed (DE) lncRNAs and 1082 DE mRNAs were identified in the porcine PBMCs after LPS stimulation. Functional enrichment analysis on DE mRNAs indicated that these genes were involved in inflammation-related signaling pathways, including cytokine-cytokine receptor interaction, TNF, NF-κB, Jak-STAT and TLR signaling pathways. Additionally, co-expression network and function analysis identified the potential lncRNAs related to inflammatory response and immune response. The expression of eight lncRNAs (ENSSSCT00000045208, ENSSSCT00000051636, ENSSSCT00000049770, ENSSSCT00000050966, ENSSSCT00000047491, ENSSSCT00000049750, ENSSSCT00000054262 and ENSSSCT00000044651) was validated in the LPS- treated and non-treated PBMCs by quantitative real-time PCR (qRT-PCR). In LPS-challenged piglets, we identified that three lncRNAs (ENSSSCT00000051636, ENSSSCT00000049770, and ENSSSCT00000047491) were significantly up-regulated in liver, spleen and jejunum tissues after LPS challenge, which revealed that these lncRNAs might be important regulators for inflammation .Conclusion: This study provides the first lncRNA and mRNA transcriptomic landscape of LPS-mediated changes in porcine PBMCs. These findings may provide potential insights into lncRNAs involved in regulating immune and inflammation in pigs.

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Section: Discussionmentioning

confidence: 95%

Construction and Analysis for Dysregulated lncRNAs and mRNAs in Lipopolysaccharide-Induced Porcine Peripheral Blood Mononuclear Cells

Zhang

Chen

et al. 2020

Preprint

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show abstract

“…Previous studies have demonstrated LPS challenge can induce severe inflammation in the piglet model, causing liver injury, intestinal damage and histological changes of spleen. 42 , 46 In liver tissues, lncRNA ENSSSCT00000047491 expression increased approximately 4- to 33-fold, lncRNA ENSSSCT00000049770 increased 4- to 12-fold and lncRNA ENSSSCT00000051636 increased 9- to 64-fold in response to LPS challenge. In the spleen and jejunum, LPS challenge induced an increase in expression of these lncRNAs with a maximum response of 4-fold increase.…”

Section: Discussionmentioning

confidence: 99%

Construction and analysis for dys-regulated lncRNAs and mRNAs in LPS-induced porcine PBMCs

Zhang

Chen

et al. 2021

Innate Immun

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Long non-coding RNAs (lncRNAs) are emerging as key regulators in inflammation. However, their functions and profiles in LPS-induced inflammation in pigs are largely unknown. In this study, we profiled global lncRNA and mRNA expression changes in PBMCs treated with LPS using the lncRNA-seq technique. In total 43 differentially expressed (DE) lncRNAs and 1082 DE mRNAs were identified in porcine PBMCs after LPS stimulation. Functional enrichment analysis on DE mRNAs indicated these genes were involved in inflammation-related signaling pathways, including cytokine–cytokine receptor interaction, TNF-α, NF-κB, Jak-STAT and TLR signaling pathways. In addition, co-expression network and function analysis identified the potential lncRNAs related to inflammatory response and immune response. The expressions of eight lncRNAs (ENSSSCT00000045208, ENSSSCT00000051636, ENSSSCT00000049770, ENSSSCT00000050966, ENSSSCT00000047491, ENSSSCT00000049750, ENSSSCT00000054262 and ENSSSCT00000044651) were validated in the LPS-treated PBMCs by quantitative real-time PCR (qRT-PCR). In LPS-challenged piglets, we identified that expression of three lncRNAs (ENSSSCT00000051636, ENSSSCT00000049770, and ENSSSCT00000047491) was significantly up-regulated in liver, spleen and jejunum tissues after LPS challenge, which indicated that these lncRNAs might be important regulators for inflammation. This study provides the first lncRNA and mRNA transcriptomic landscape of LPS-mediated changes in porcine PBMCs, which might provide potential insights into lncRNAs involved in regulating inflammation in pigs.

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“…These results suggest that the humoral immune response was enhanced by COPE. It has been suggested that prominent germinal centers are indicative of increases in the population of proliferating B-lymphocytes and stimulation of humoral response [11,12].…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory properties of ethanol extract of <i>Canarium ovatum</i> (Burseraceae) pulp

Salvador-Membreve¹,

Cajuday²,

Serrano³

et al. 2018

Trop. J. Pharm Res

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Purpose:To evaluate the immunomodulatory properties of ethanol extract of the pulp of Canarium ovatum (COPE). Methods: The immunomodulatory activity of ethanolic extract of the pulp of C. ovatum was investigated in vivo using Balb/C mice. Extract doses of 300 and 600 mg/kg were orally administered to study its effect on delayed type hypersensitivity and humoral antibody response using sheep red blood cells (SRBC). Acute oral toxicity profile and phytochemical analysis were also determined. Results: Orally -administered COPE did not exhibit any mortality or signs of toxicity at doses 300 -2000 mg/kg. Phytochemical analysis revealed the presence of biologically-active compounds such as sterols, triterpenes, flavonoids, alkaloids, saponins, glycosides and tannins. Treatment with COPE for 7 days stimulated the early phase of DTH response through significant increase in foot pad thickness (111.87 ± 9.97 % at 300 mg/kg, and 91.27 ± 7.81 % at 600 mg/kg), when compared to distilled water and cyclophosphamide (CP) groups. Similarly, COPE significantly enhanced antibody titer, with highest titer at the dose of 300 mg/kg. Histological observations of the spleen showed follicles with active germinal centers and proliferating lymphocytes, which are consistent with the immunostimulatory effects of COPE. Conclusion: These results show that COPE has stimulatory effects on cellular and humoral responses in mice, indicating its potential as an immunostimulatory agent. This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest

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Distribution and Expression of Visfatin-Positive Cells in the Spleen of Lipopolysaccharide-Stimulated Piglets

Cited by 4 publications

References 23 publications

Construction and Analysis for Dysregulated lncRNAs and mRNAs in Lipopolysaccharide-Induced Porcine Peripheral Blood Mononuclear Cells

Construction and Analysis for Dysregulated lncRNAs and mRNAs in Lipopolysaccharide-Induced Porcine Peripheral Blood Mononuclear Cells

Construction and analysis for dys-regulated lncRNAs and mRNAs in LPS-induced porcine PBMCs

Immunomodulatory properties of ethanol extract of <i>Canarium ovatum</i> (Burseraceae) pulp

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