Distribution and expression of SLC45A2 in the skin of sheep with different coat colors

Wang, Haidong; Li, Xue; Li, Yanan; Zhao, Bingling; Chen, Tianzhi; Liu, Ying; Chang, Lucheng; Wang, Juan

doi:10.5603/fhc.a2016.0015

Cited by 9 publications

(7 citation statements)

References 34 publications

(46 reference statements)

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“…In our study, the SLC45A2 gene was enriched in the cellular component, melanosome membrane; and in the two BP, melanin biosynthetic process and developmental pigmentation. The expression levels of the SLC45A2 gene were up-regulated in the cyan gray and black groups, which was consistent with studies in small-tailed Han sheep (Wang et al, 2016) and with the results of our RNA-seq analysis.…”

Section: Discussionsupporting

confidence: 92%

Transcriptome profiling analysis reveals key genes of different coat color in sheep skin

Yao¹,

Bao²,

Hong³

et al. 2019

PeerJ

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BackgroundTo investigate the molecular mechanisms determining the coat color of native breed sheep in Xinjiang.MethodsBashibai sheep, Yemule white sheep and Tulufan black sheep were selected. Illumina HiSeq X Ten sequencing technology was used to detect the genes responsible for the white, light brown, black and cyan gray coat colors in sheep. Sequence analysis and functional gene annotation analysis were performed to analyze the results. The signal pathways and differentially expressed genes related to sheep hair color production regulation were screened and finally verified by real-time polymerase chain reaction.ResultsFunctional annotation by Kyoto Encyclopedia of Genes and Genomes analysis revealed significant differences in enrichment of immunity-related pathways as well as melanogenesis synthetic and tyrosine metabolism pathways. Our results showed that the DCT, TYR, TYRP1, PMEL, SLC45A2 and MLANA six genes may be associated with the regulation of coat color development and provide a theoretical basis for selecting natural coat colors of sheep.

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Section: Discussionsupporting

confidence: 92%

Transcriptome profiling analysis reveals key genes of different coat color in sheep skin

Yao¹,

Bao²,

Hong³

et al. 2019

PeerJ

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“…Skin pigmentation in animals is a polygenic trait, and may be influenced by different kinds of multi-gene interactions ( Barsh, 1996 ). The proteins and genes associated with skin color in humans, mice, chickens, and sheep include agouti signaling protein (ASIP) ( Lu et al, 1994 ), melanocyte-stimulating hormone receptor (MSH) ( Robbins & Nadeau, 1993 ), microphthalmia transcription factor (MITF) ( Dong, Yoshiaki & Vijayasaradhi, 2002 ; Vetrini et al, 2004 ; Wang et al, 2018 ), tyrosinase (TYR) ( Deng et al, 2008 ; Yu et al, 2017 ), tyrosinase-related protein-1 (TYRP1) ( Chun et al, 2016 ), dopachrome tautomerase (DCT) ( Xue et al, 2018 ), melanocortin 1 receptor (MC1R) ( Fontanesi et al, 2010 ; Bourgeois et al, 2016 ; Wolf Horrell, Boulanger & D’Orazio, 2016 ), solute carrier family 45 member 2 (SLC45A2) ( Wang et al, 2016 ), and others. There are over 100 known color loci in mice and many of them have been cloned and sequenced ( Bennett & Lamoreux, 2003 ).…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome and histological analyses provide insights into the skin pigmentation in Minxian black fur sheep (Ovis aries)

Shi

Xia

et al. 2021

PeerJ

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Background Minxian black fur (MBF) sheep are found in the northwestern parts of China. These sheep have developed several special traits. Skin color is a phenotype subject to strong natural selection and diverse skin colors are likely a consequence of differences in gene regulation. Methods Skin structure, color differences, and gene expression (determined by RNA sequencing) were evaluated the Minxian black fur and Small-tail Han sheep (n = 3 each group), which are both native Chinese sheep breeds. Results Small-tail Han sheep have a thicker skin and dermis than the Minxian black fur sheep (P < 0.01); however, the quantity of melanin granules is greater (P < 0.01) in Minxian black fur sheep with a more extensive distribution in skin tissue and hair follicles. One hundred thirty-three differentially expressed genes were significantly associated with 37 ontological terms and two critical KEGG pathways for pigmentation (“tyrosine metabolism” and “melanogenesis” pathways). Important genes from those pathways with known involvement in pigmentation included OCA2 melanosomal transmembrane protein (OCA2), dopachrome tautomerase (DCT), tyrosinase (TYR) and tyrosinase related protein (TYRP1), melanocortin 1 receptor (MC1R), and premelanosome protein (PMEL). The results from our histological and transcriptome analyses will form a foundation for additional investigation into the genetic basis and regulation of pigmentation in these sheep breeds.

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“…We have found that many growth factors and genes regulate melanogenesis. For example, we found that ocular albinism type 1 protein (OA1) and glycoprotein nonmetastatic melanoma protein b (GPNMB) regulate melanogenesis in mouse melanocytes; the expression of slc45a2 and endothelin 3 are also related to sheep coat colors [4][5][6][7]; cyclin-dependent kinase 5 could regulate melanogenesis and epidermal structures [8]; and FGF21 regulates the pigmentation of alpaca melanocytes by the ERK1/2 pathway [9]. Moreover, IGF1 can modulate alpaca melanogenesis by the cAMP pathway [10].…”

Section: Introductionmentioning

confidence: 99%

Exosomal miRNA derived from keratinocytes regulates pigmentation in melanocytes

Liu

Gao

et al. 2019

Journal of Dermatological Science

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Background: Pigmentation is controlled by complex mechanisms. Evidence suggests that miRNAs can regulate pigmentation. However, the mechanism has not been fully elucidated. Objective In this study, we revealed a novel mechanism that regulates pigmentation involving exosomes, miRNAs and the crosstalk between keratinocytes and melanocytes. Methods: The expression and localization of exosome specific marker TSG101 in keratinocytes and melanocytes; Changes of melanin content in melanocytes after co-culture of exosome and melanocytes; Expression changes of target gene TYR and its related genes and inhibitory effect of miR-330-5p on pigmentation were studied by using various molecular biological techniques. Results: In this experiment, we used miR-330-5p in keratinocytes to verify the effect of keratinocyte derived exosome on melanocyte pigmentation. First, we found that keratinocytes secrete exosomes carrying miR-330-5p; moreover, greater miR-330-5p expression was found in exosomes derived from keratinocytes that overexpressed miR-330-5p. Second, we found that exosomes derived from keratinocytes with overexpression of miR-330-5p caused a significant increase in miR-330-5p in melanocytes. Finally, exosomes derived from keratinocytes that overexpressed miR-330-5p induced a significant decrease in the production of melanin and expression of TYR in melanocytes. Meanwhile, we overexpressed miR-330-5p in melanocytes, which also proved the inhibitory effect of miR-330-5p on pigmentation. Conclusion: These findings suggest that keratinocytes crosstalk with melanocytes in the epidermal melanin unit via exosomal miRNAs. These studies reveal an important role of exosomes in melanocyte pigmentation, which opens a new pathway of melanogenesis.

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Distribution and expression of SLC45A2 in the skin of sheep with different coat colors

Cited by 9 publications

References 34 publications

Transcriptome profiling analysis reveals key genes of different coat color in sheep skin

Transcriptome profiling analysis reveals key genes of different coat color in sheep skin

Comparative transcriptome and histological analyses provide insights into the skin pigmentation in Minxian black fur sheep (Ovis aries)

Exosomal miRNA derived from keratinocytes regulates pigmentation in melanocytes

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