Distribution and effects of polymorphic RANTES gene alleles in HIV/HCV coinfection – A prospective cross-sectional study

Ahlenstiel, Golo; Iwan, Agathe; Nattermann, Jacob; Bueren, Karin; Rockstroh, Jürgen K.; Brackmann, H.‐H.; Kupfer, Bernd; Landt, Olfert; Peled, Amnon; Sauerbruch, Tilman; Spengler, Ulrich; Woitas, Rainer P.

doi:10.3748/wjg.v11.i48.7631

Cited by 15 publications

(11 citation statements)

References 24 publications

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“…The frequency of the RANTES In1.1C allele in this study population (20%) is lower than what has been observed among Asians (28%) [15], but higher than that among Caucasians (10%) [31]. The occurrence of the RANTES -403A allele in Zimbabweans (44%) was higher than that among Caucasians (16%) [32] but was comparable to that observed among Asians (40%) [33]. The RANTES In1.1T>C polymorphism is located in an intronic regulatory sequence and the In1.1C variant is associated with down-regulation of RANTES expression [34], resulting in fewer chemokines available to inhibit HIV binding or to internalize CCR5 receptors on T-cells, thus favoring HIV infection.…”

Section: Chemokine Receptor Variants and Their Association With Hiv Icontrasting

confidence: 41%

CCR2, CX3CR1, RANTES and SDF1 genetic polymorphisms influence HIV infection in a Zimbabwean pediatric population

Mhandire

Duri

Kandawasvika

et al. 2014

J Infect Dev Ctries

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Introduction: There is growing evidence that polymorphisms in chemokine and chemokine receptor genes influence susceptibility to HIV infection and disease progression. However, not much is documented about the prevalence and effects of chemokine and chemokine receptor gene variations in the Zimbabwean population despite the high burden of HIV/AIDS in the country. This study therefore describes polymorphisms in CCR2, CX3CR1, SDF1 and RANTES genes in a Zimbabwean pediatric population and their effects on HIV infection in children born to HIV-infected mothers. Methodology: A total of 106 children between seven and nine years of age comprising 70 perinatally exposed to HIV (34 born infected [EI] and 36 born uninfected [EU]) and 36 unexposed and uninfected (UEUI) controls were recruited. Six allelic variants in four genes were genotyped using PCR-RFLP and sequencing. Results: Frequencies for minor alleles in the HIV uninfected groups (EU and UEUI) were CCR2 190A (16%), SDF1 801A (2%), CX3CR1 745A (9%), CX3CR1 839T (0%), RANTES In 1.1C (20%), and RANTES -403A (44%). There were significant differences between the EI and EU groups in the distribution of CCR2 190G/A genotype (15% versus 39%, respectively, p = 0.02) and CCR2 190G/A-CX3CR1 745G/G genotype combination (0% versus 33%, respectively, p = 0.002). Conclusions: Our findings suggest that chemokine and chemokine receptor gene variants seem to play an important role in the dynamics of HIV infection and could be used as drug or vaccine targets.

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Section: Chemokine Receptor Variants and Their Association With Hiv Icontrasting

confidence: 41%

CCR2, CX3CR1, RANTES and SDF1 genetic polymorphisms influence HIV infection in a Zimbabwean pediatric population

Mhandire

Duri

Kandawasvika

et al. 2014

J Infect Dev Ctries

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“…The two CCL5 promoter polymorphisms, −403G/A and −28C/G, have been shown to be associated with many infectious diseases such as HIV, hepatitis and TB, (An et al. , 2002; Ahlenstiel et al. , 2005; Chu et al.…”

Section: Introductionmentioning

confidence: 99%

CCL5 (RANTES) gene polymorphisms in pulmonary tuberculosis patients of south India

Selvaraj

Alagarasu

Singh

et al. 2011

International Journal of Immunogenetics

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The chemokine CCL5 is known to play an important role in the formation of granuloma during infection with Mycobacterium tuberculosis. Production of CCL5 is influenced by polymorphisms in the CCL5 gene. Hence, in the present study, we investigated whether polymorphisms in the promoter and intron regions of CCL5 gene are associated with susceptibility or resistance to pulmonary tuberculosis in south Indian population. Polymorphisms in the promoter (-403G/A and -28C/G) and intron (In1.1T/C) regions of CCL5 gene were studied in 212 pulmonary tuberculosis (PTB) patients and 213 healthy controls (HCs). Allele and genotype frequencies of CCL5 gene polymorphisms were not different between PTB patients and HCs. When the haplotype and diplotype frequencies were compared, a significantly decreased frequencies of the haplotype A-C-C [P = 0.037; Odds ratio (OR): 0.57; 95% confidence interval (CI): 0.34-0.97] and the diplotype G/A-T/C (P = 0.017; OR: 0.46; 95% CI: 0.24-0.88) were observed among PTB patients when compared with HCs. However, the significant differences observed for the haplotype and the diplotype were lost when corrected for multiple comparisons [Bonferroni correction: A-C-C P corrected (P(c) ) = 0.148 and G/A-T/C P(c) = 0.136]. Though the present results suggest that the CCL5 gene haplotype A-C-C and the diplotype G/A-T/C may be associated with resistance to PTB, further studies with increased sample size may be useful to confirm this present finding as well as to understand the role of CCL5 haplotype and diplotype on genetic susceptibility to TB.

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“…Genotyping of RANTES In1.1C was carried out by previously described protocol [Ahlenstiel et al, 2005] using forward primer: 5 0 -CCT GGT CTT GAC CAC CAC A-3 0 and reverse primer: 5 0 -GCT GAC AGG CAT GAG TCA GA-3 0 . A 343-bp fragment spanning position In1.1C was amplified from extracted genomic DNA.…”

Section: Polymerase Chain Reaction and Sequencingmentioning

confidence: 99%

Association of RANTES −403 G/A, −28 C/G and In1.1 T/C polymorphism with HIV‐1 transmission and progression among North Indians

Rathore

Chatterjee

Sivarama³

et al. 2008

Journal of Medical Virology

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The relationships between host immune factors and HIV-1 disease progression are still in dispute. The RANTES SNPs exhibit distinct ethnic distribution and are associated with different effects on the course of HIV infection. Therefore, impact of RANTES gene polymorphism on HIV-1 transmission and progression needs to be evaluated. The RANTES genotypes were identified by PCR-RFLP method and confirmed by sequencing in HIV-1 seronegative (HSN; n=315), HIV-1 exposed seronegative (HES; n=47) and HIV-1 seropositive (HSP; n=196) patients classified into different clinical stages (i.e. Stages I, II, III). Fisher exact test was used for statistical analysis and Arlequin software for haplotype analysis. RANTES allele -403G, -28C and In1.1 T were the predominant allele in the subject studied. HSP group have higher frequency of RANTES In1.1 T allele compared with HSN (91.32% vs. 86.19%; P=0.013) and HES (91.32% vs. 78.72%; P=0.001). Higher frequency of RANTES In1.1 C allele in Stage III was observed, compared with Stage I (14.28% vs. 6.39%) and was significantly associated with high risk (P=0.047, OR=2.439, C.I.=1.061-5.609). Haplotype II (ACT) was significantly higher in HSP compared with HSN (9.69% vs. 1.58%) and associated with high risk (P<0.001, OR=6.655, C.I.=2.443-18.132). There were no significant differences in RANTES -403 A/G and -28 C/G genotype and allele distribution in all the groups compared. Our results implicate that RANTES In1.1 T allele and haplotype II (ACT) may be a risk factor for HIV-1 transmission while RANTES In1.1 C allele may be risk factor for disease progression among North Indians.

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Distribution and effects of polymorphic RANTES gene alleles in HIV/HCV coinfection – A prospective cross-sectional study

Cited by 15 publications

References 24 publications

CCR2, CX3CR1, RANTES and SDF1 genetic polymorphisms influence HIV infection in a Zimbabwean pediatric population

CCR2, CX3CR1, RANTES and SDF1 genetic polymorphisms influence HIV infection in a Zimbabwean pediatric population

CCL5 (RANTES) gene polymorphisms in pulmonary tuberculosis patients of south India

Association of RANTES −403 G/A, −28 C/G and In1.1 T/C polymorphism with HIV‐1 transmission and progression among North Indians

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