Distribution and dynamics of epidemic and pandemic Vibrio parahaemolyticus virulence factors

Ceccarelli, Daniela; Hasan, Nur A.; Huq, Anwar; Colwell, Rita R.

doi:10.3389/fcimb.2013.00097

Cited by 174 publications

(155 citation statements)

References 103 publications

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“…While most strains are nonpathogenic, many have acquired virulence factors that result in illness when individuals are exposed to V. parahaemolyticus strains carrying these virulence factors (1). V. parahaemolyticus is recognized to be a leading cause of foodborne illness worldwide and is transmitted via the handling and consumption of raw or undercooked contaminated seafood (1).…”

mentioning

confidence: 99%

Genomic Features of Environmental and Clinical Vibrio parahaemolyticus Isolates Lacking Recognized Virulence Factors Are Dissimilar

Ronholm

Petronella

Leung

et al. 2016

Appl Environ Microbiol

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Vibrio parahaemolyticus is a bacterial pathogen that can cause illness after the consumption or handling of contaminated seafood. The primary virulence factors associated with V. parahaemolyticus illness are thermostable direct hemolysin (TDH) and Tdh-related hemolysin (TRH). However, clinical strains lacking tdh and trh have recently been isolated, and these clinical isolates are poorly understood. To help understand the emergence of clinical tdh-and trh-negative isolates, a genomic approach was used to comprehensively compare 4 clinical tdh-and trh-negative isolates with 16 environmental tdh-and trh-negative isolates and 34 clinical isolates positive for tdh or trh, or both, with the objective of identifying genomic features that are unique to clinical tdh-and trh-negative isolates. The prevalence of pathogenicity islands (PAIs) common to clinical isolates was thoroughly examined in each of the clinical tdh-and trh-negative isolates. The tdh PAI was not present in any clinical or environmental tdhand trh-negative isolates. The trh PAI was not present in any environmental isolates; however, in clinical tdh-and trh-negative isolate 10-4238, the majority of the trh PAI including a partial trh1 gene was present, which resulted in reclassification of this isolate as a tdh-negative and trh-positive isolate. In the other clinical tdh-and trh-negative isolates, neither the trh gene nor the trh PAI was present. We identified 862 genes in clinical tdh-and trh-negative isolates but not in environmental tdh-and trh-negative isolates. Many of these genes are highly homologous to genes found in common enteric bacteria and included genes encoding a number of chemotaxis proteins and a novel putative type VI secretion system (T6SS) effector and immunity protein (T6SS1). The availability of genome sequences from clinical V. parahaemolyticus tdh-and trh-negative isolates and the comparative analysis may help provide an understanding of how this pathotype is able to survive in vivo during clinical illness. Vibrio parahaemolyticus is a Gram-negative, halophilic bacterium that is ubiquitous in marine and estuarine environments and is often found colonizing shellfish or shrimp. While most strains are nonpathogenic, many have acquired virulence factors that result in illness when individuals are exposed to V. parahaemolyticus strains carrying these virulence factors (1). V. parahaemolyticus is recognized to be a leading cause of foodborne illness worldwide and is transmitted via the handling and consumption of raw or undercooked contaminated seafood (1). Infections occur both sporadically and in very large outbreaks. The most common manifestation of V. parahaemolyticus infection is acute, watery diarrhea accompanied by abdominal pain and nausea, although symptoms can also be severe and include a dysenterylike illness or septicemia (2). Since most cases of illness caused by V. parahaemolyticus are self-limiting, rates of infection are probably underestimated due to underreporting.Clinical V. parahaemolyticus isolates generally have at...

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confidence: 99%

Genomic Features of Environmental and Clinical Vibrio parahaemolyticus Isolates Lacking Recognized Virulence Factors Are Dissimilar

Ronholm

Petronella

Leung

et al. 2016

Appl Environ Microbiol

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“…yang bersifat patogen terhadap komoditas udang maupun manusia (Ceccarelli, Hasan, Hug, & Colwell, 2013;DePaola Kaysner, Bowers, & Cook, 2000). Bakteri ini terdapat secara alami di lingkungan perairan dan seringkali dijumpai di lingkungan budidaya udang (Abu & Egenonu, 2008;Chitov, Wongdao, Thatum, Puprae, & Sisuwan, 2009;Newton, Kendall, Vugia, Henao, & Maho, 2012).…”

Section: Pendahuluanunclassified

RESISTENSI ANTIBIOTIK PADA Vibrio parahaemolyticus DARI UDANG VANAME ASAL PANTAI UTARA JAWA UNTUK PASAR EKSPOR

Kusmarwati

Yenni

Indriati

2017

JPBKP

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ABSTRAKKeberadaan bakteri V. parahaemolyticus pada produk udang yang bersifat resisten terhadap antibiotik saat ini menjadi permasalahan serius yang berdampak pada jaminan mutu dan keamanan produk. Hal ini menjadi ancaman serius bagi manusia ketika mengkonsumsi udang tersebut. Penelitian ini bertujuan untuk mengetahui resistensi antibiotik dan potensi risiko antibiotik dari bakteri V. parahaemolyticus pada udang vaname. Pengambilan sampel udang vaname segar dari tambak dilakukan pada musim hujan dan musim kemarau di wilayah Pantai Utara Jawa pada bulan Februari hingga Oktober 2015 dengan metode purposive random sampling. Sebanyak 36 isolat bakteri V. parahaemolyticus yang mewakili 103 sampel udang vaname segar dari tambak udang di Jawa Barat, Jawa Tengah dan Jawa Timur telah diuji resistensinya terhadap 8 jenis antibiotik (doksisiklin, nitrofurantoin, siprofloksasin, asam nalidiksat, amoksisilin-asam klavulanat, kloramfenikol, streptomisin, dan eritromisin). Uji kepekaan bakteri terhadap antibiotik dilakukan menggunakan metode Kirby-Bauer. Perhitungan indeks Multiple Antibiotic Resistance (MAR) juga dilakukan untuk mengetahui potensi risiko antibiotik terhadap kesehatan manusia. Hasil analisis menunjukkan bahwa 100% isolat bakteri yang diuji resisten terhadap streptomisin, 90% isolat resisten terhadap eritromisin, dan berikutnya resisten terhadap amoksisilin-asam klavulanat dan nitrofurantoin masing-masing sebesar 83,33% dan 58,33%. Terdapat beberapa antibiotik yang masih mampu melawan bakteri V. parahaemolyticus yaitu siprofloksasin (88,89%), kloramfenikol (81,25%) dan doksisiklin (33,33%). Selain itu, sebanyak 63,89% dari total isolat bakteri tersebut memiliki indeks MAR>0,2 yang mengindikasikan adanya potensi risiko bagi kesehatan. Secara keseluruhan, bakteri V. parahaemolyticus dari sampel yang diambil pada musim hujan menunjukkan resistensi yang lebih tinggi. (doxycycline, nitrofurantoin, ciprofloxacin, nalidixic acid, amoxicillin-clavulanic acid, chloramphenicol, streptomycin, and erythromycin KATA KUNCI : multiple antibiotic resistance, udang, tambak, kloramfenikol ABSTRACT Recently, the occurence of V. parahaemolyticus bacteria in shrimp products that was resistant to antibiotics became a serious problem that affects quality assurance and product safety. It will be a serious threat to humans when consuming the shrimp product. Research was conducted to know antibiotic resistance and antibiotic risk potency of V. parahaemolyticus bacteria on vannamei shrimp. Sampling of fresh vannamei shrimp from the ponds was conducted in rainy and dry season in the Northern Coast of Java from February to October 2015 with purposive randomize sampling method. A total of 36 isolates of V. parahaemolyticus bacteria representing 103 samples of fresh vannamei shrimp from shrimp ponds in West Java, Central Java and East Java have been tested for resistance to 8 types of antibiotics

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“…The presence of this bacterium in the marine environment raises the concern of human on food safety due to the latter potential in causing disease outbreaks depending on the environmental conditions (Ceccarelli et al, 2013).…”

Section: Issn: 2319-7706 Volume 6 Number 6 (2017) Pp 2760-2778mentioning

confidence: 99%

Phenotypic Characterization of Vibrio Species: Application of Indigenous Phages for Biological Control of Vibrio in Aquaculture Live Feeds

Hassan¹

2017

Int.J.Curr.Microbiol.App.Sci

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Distribution and dynamics of epidemic and pandemic Vibrio parahaemolyticus virulence factors

Cited by 174 publications

References 103 publications

Genomic Features of Environmental and Clinical Vibrio parahaemolyticus Isolates Lacking Recognized Virulence Factors Are Dissimilar

Genomic Features of Environmental and Clinical Vibrio parahaemolyticus Isolates Lacking Recognized Virulence Factors Are Dissimilar

RESISTENSI ANTIBIOTIK PADA Vibrio parahaemolyticus DARI UDANG VANAME ASAL PANTAI UTARA JAWA UNTUK PASAR EKSPOR

Phenotypic Characterization of Vibrio Species: Application of Indigenous Phages for Biological Control of Vibrio in Aquaculture Live Feeds

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