Distribution and Diversity of Beauveria in Boreal Forests of Northern European Russia

Казарцев, И. А.; Lednev, Georgy R

doi:10.3390/microorganisms9071409

Cited by 5 publications

(14 citation statements)

References 62 publications

(80 reference statements)

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“…The ability of B. caledonica to cause lethal infections on C. sordidus adults and to inhibit Foc conidia germination suggests new possibilities for managing both the pest and the plant pathogen, which may also interact as a complex in banana plantations. 4 The fungus B. caledonica was first isolated from soil in Scotland, 38 and later from bark beetles (Scolytinae) in New Zealand, 39 Ireland 40 and Russia 41 . In Brazil, this species was recently found infecting C. sordidus adults in the mid-west of the country.…”

Section: Discussionmentioning

confidence: 99%

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Natural occurrence of Beauveria caledonica, pathogenicity to Cosmopolites sordidus and antifungal activity against Fusarium oxysporum f. sp. cubense

Mascarin

Marinho-Prado

Assalin

et al. 2022

Pest Management Science

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BACKGROUND: Entomopathogenic fungi can provide a set of ecological services, such as suppressing arthropod pests and plant pathogens. In this study, novel indigenous Beauveria caledonica (Bc) strains were isolated from naturally infected banana weevils (Cosmopolites sordidus) occurring in commercial banana plantations in Brazil.RESULTS: The prevalence of infection by Bc strains on field-caught C. sordidus ranged from 1.3% to 12.9%. Similar to the Beauveria bassiana strains tested, none of the Bc strains caused more than 50% weevil mortality at a concentration of 1 × 10 8 conidia ml −1 . Bc strain CMAA1810 caused the highest mortality in C. sordidus and had enhanced insecticidal activity when formulated with an emulsifiable oil. In paired co-culture assays, this same strain showed a significant growth-inhibitory effect on the causal agent of Fusarium banana wilt (Fusarium oxysporum f. sp. cubense, Foc) of twofold magnitude compared with the control. Cell-free crude filtrates derived from the red-pigmented culture broth of Bc (CMAA1810) strongly reduced Foc conidial viability, and this inhibitory activity was inversely related to the age of the Bc culture. Crude concentrated filtrates from 4-dayold cultures exhibited the strongest antifungal activity (13-fold) compared with untreated Foc conidia. The abundant compound identified in the crude filtrate of Bc was oosporein (1,4-dibenzoquinone) present at a concentration of 0.829 ± 0.018 mg g −1 dry matter, and the antifungal activity of the filtrate was demonstrated.CONCLUSION: These results indicated that Bc strains might have the potential to manage both C. sordidus and Foc, two of the major phytosanitary problems in banana crops worldwide. Further research under field conditions using suitable formulations of virulent Bc strains in combination with the metabolite oosporein is needed to evaluate their efficacy in the management of C. sordidus and Foc in banana plantations.

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Section: Discussionmentioning

confidence: 99%

“…The fungus B. caledonica was first isolated from soil in Scotland, 38 and later from bark beetles (Scolytinae) in New Zealand, 39 Ireland 40 and Russia 41 . In Brazil, this species was recently found infecting C. sordidus adults in the mid‐west of the country 22 .…”

Section: Discussionmentioning

confidence: 99%

Natural occurrence of Beauveria caledonica, pathogenicity to Cosmopolites sordidus and antifungal activity against Fusarium oxysporum f. sp. cubense

Mascarin

Marinho-Prado

Assalin

et al. 2022

Pest Management Science

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“…Deoxyribonucleic Acid (DNA) isolation and amplification of the Thyrotroph Embryonic Factor (TEF) region. The Cetyltrimethylammonium Bromide (CTAB) method was used for isolation (Kazartsev and Lednev, 2021), where the buffer solution contained 100 mM Tris-HCl, pH 8.0, 1.4 M NaCl, 20 mm Ethylenediamine Tetraacetic Acid (EDTA), 2% CTAB and 100 g/mL of proteinase K. The Polymerase Chain Reaction (PCR) (Kazartsev and Lednev, 2021) was performed with primers TEF 1-f (5'-GCTCCCGGTCACCTGAYTTYAT-3' and TEF 1-r (5'-ATGACACCCACAGGACGGTCTG-3').…”

Section: Methodsmentioning

confidence: 99%

“…Sampling from Culture Liquid (CL) was carried out every 3 days. The blastospore titer was calculated according to the standard method using the Goryaev chamber (Kazartsev and Lednev, 2021).…”

Section: Methodsmentioning

confidence: 99%

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Effective Strains of Beauveria Bassiana and B. Pseudobassiana used Against the Asian Locust (Locusta Migratoria L.) in Kazakhstan

Zhumataуeva¹,

Duissembekov²,

Dutbayev³

et al. 2022

OnLine Journal of Biological Sciences

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The purpose of the study was to perform genetic identification of the strains of fungi of the Beauveria genus and evaluate the effect of abiotic factors on the growth and survival of colonies and the productivity of conidia of these fungi, as well as to determine the biological effectiveness of the semi-preparative form of the drug based on Beauveria bassiana in the field. Conventional and modern methods accepted in biology and mycology were used. Identification of strains of Beauveria fungi was carried out by determining the direct nucleotide sequence of the TEF region. Laboratory experiments were carried out to evaluate various factors on the diameter of colony growth, survival in deep and surface cultivation, the type of semi-preparative form of the drug based on B. bassiana and the time of its exposure, and the effect on the biological effects of drugs in the field. Statistical data processing was carried out in the R studio program using the P value. The productivity of conidia of entomopathogenic fungi during surface cultivation depended on the type of strain and the duration of cultivation of conidia. The diameter of the growth of Beauveria fungi colonies was influenced by the factors of the strain, the type of nutrient medium, the temperature of colony cultivation, and the number of days after the start of colony cultivation. The survival of conidia of the B. bassiana fungus on the wings of Locusta migratoria L. orrelated with the indicators of cultivation temperature and germination of conidia and cultivation time. The productivity of Beauveria spp. conidia correlate with the type of strain, the type of substrate, and the time of exposure. The conidia continued to germinate on the 25 th day. Fungi grew better on a substrate based on millet. In the field, the mortality rate of L. migratoria L. and the biological effectiveness of biopesticides were influenced by the factors of the strain, the form of spraying with the bio preparation, and the time elapsed after treatment with the biopesticide.

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Recombinant Beauveria bassiana expressing Bacillus thuringiensis toxin Cyt1Aa: a promising approach for enhancing Aedes mosquito control

Deng,

Li,

Yang

et al. 2024

Microbiol Spectr

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The entomopathogenic fungus Beauveria bassiana provides an eco-friendly substitute to chemical insecticides for mosquito control. Nevertheless, its widespread application has been hindered by its comparatively slow efficacy in eliminating mosquitoes. To augment the potency of B. bassiana against Aedes mosquitoes, a novel recombinant strain, Bb -Cyt1Aa, was developed by incorporating the Bacillus thuringiensis toxin gene Cyt1Aa into B. bassiana . The virulence of Bb -Cyt1Aa was evaluated against Aedes aegypti and Aedes albopictus using insect bioassays. Compared to the wild-type (WT) strain, the median lethal time (LT 50 ) for A. aegypti larvae infected with Bb -Cyt1Aa decreased by 33.3% at a concentration of 1 × 10 8 conidia/mL and by 22.2% at 1 × 10 7 conidia/mL. The LT 50 for A. aegypti adults infected with Bb -Cyt1Aa through conidia ingestion was reduced by 37.5% at 1 × 10 8 conidia/mL and by 33.3% at 1 × 10 7 conidia/mL. Likewise, the LT 50 for A. aegypti adults infected with Bb -Cyt1Aa through cuticle contact decreased by 33.3% and 30.8% at the same concentrations, respectively. Furthermore, the Bb -Cyt1Aa strain also demonstrated increased toxicity against both larval and adult A. albopictus , when compared to the WT strain. In conclusion, our study demonstrated that the expression of B. thuringiensis toxin Cyt1Aa in B. bassiana enhanced its virulence against Aedes mosquitoes. This suggests that B. bassiana expressing Cyt1Aa has potential value for use in mosquito control. IMPORTANCE Beauveria bassiana is a naturally occurring fungus that can be utilized as a bioinsecticide against mosquitoes. Cyt1Aa is a delta-endotoxin protein produced by Bacillus thuringiensis that exhibits specific and potent insecticidal activity against mosquitoes. In our study, the expression of this toxin Cyt1Aa in B. bassiana enhances the virulence of B. bassiana against Aedes aegypti and Aedes albopictus , thereby increasing their effectiveness in killing mosquitoes. This novel strain can be used alongside chemical insecticides to reduce dependence on harmful chemicals, thereby minimizing negative impacts on the environment and human health. Additionally, the potential resistance of B. bassiana against mosquitoes in the future could be overcome by acquiring novel combinations of exogenous toxin genes. The presence of B. bassiana that expresses Cyt1Aa is of significant importance in mosquito control as it enhances genetic diversity, creates novel virulent strains, and contributes to the development of safer and more sustainable methods of mosquito control.

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Distribution and Diversity of Beauveria in Boreal Forests of Northern European Russia

Cited by 5 publications

References 62 publications

Natural occurrence of Beauveria caledonica, pathogenicity to Cosmopolites sordidus and antifungal activity against Fusarium oxysporum f. sp. cubense

Natural occurrence of Beauveria caledonica, pathogenicity to Cosmopolites sordidus and antifungal activity against Fusarium oxysporum f. sp. cubense

Effective Strains of Beauveria Bassiana and B. Pseudobassiana used Against the Asian Locust (Locusta Migratoria L.) in Kazakhstan

Recombinant Beauveria bassiana expressing Bacillus thuringiensis toxin Cyt1Aa: a promising approach for enhancing Aedes mosquito control

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