Distribution and Characterization of Dominant Serovars of Listeria Monocytogenes Strains Isolated from Spontaneous Human Abortion in Tehran

Rezaei, Maryam; Pour, Nadia Kazemi; Vandyousefi, J; Zamin, Farokh Rokhbakhsh; Irajian, Gholamreza

doi:10.18502/ijml.v5i4.158

Cited by 3 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Whereas, Kaur et al ( 11 ) found that 2 (50%) L. monocytogenes isolates carried all genes of this research plus iap gene. Rezaei et al ( 29 ) documented the existence of the hlyA gene in all L. monocytogenes isolates with spontaneous abortions. On the other hand, the presence of hlyA gene was detected in only 2 (40%) out of five L. monocytogenes isolates while 3 lacked this gene ( 30 ) probably due to mutation ( 31 ).…”

Section: Discussionmentioning

confidence: 99%

Multiple drug resistance of Listeria monocytogenes isolated from aborted women by using serological and molecular techniques in Diwaniyah city/ Iraq

Al-Mayahi¹,

Jaber²

2020

IJM

View full text Add to dashboard Cite

Background and Objectives: The study was sought to detect the effect of Listeria monocytogenes on pregnant Iraqi women at Al-Diwaniya hospitals and determination of virulence genes and antimicrobial susceptibility of isolates. Materials and Methods: 360 specimens including blood, urine, vaginal and endocervical were collected from 90 patients with spontaneous abortions. Blood samples were displayed to immunological study and remaining specimens were subjected to bacteriological diagnosis. PCR was used to determine the virulence factors and antimicrobial resistance genes. Results: Fifteen positive samples (16.6%) of patients and thirteen isolates (14.5%) from patients were recognized based on ELISA and PCR assay respectively. The general isolation of L. monocytogenes strains in cases of abortive women was 13/270 (4.8%). L. monocytogenes strains were highly virulent because of presence of virulence factors associated genes, namely actA, hlyA, plcA and prfA in all strains. Multiple drug resistance (MAR) index values of 15.4% of isolates were >0.2. Conclusion: It is necessary for conducting susceptibility testing and to select the suitable antibiotics and avoid the effects of these bacteria in pregnant women.

show abstract

Section: Discussionmentioning

confidence: 99%

Multiple drug resistance of Listeria monocytogenes isolated from aborted women by using serological and molecular techniques in Diwaniyah city/ Iraq

Al-Mayahi¹,

Jaber²

2020

IJM

View full text Add to dashboard Cite

show abstract

Extraintestinal infection of Listeria monocytogenes and susceptibility to spontaneous abortion during pregnancy: A systematic review and meta-analysis

Yousefi¹,

Karbalaei²,

Keikha³

2021

WJMA

View full text Add to dashboard Cite

Determination of Dominant Serovars and Molecular Analysis of hly and iap Genes Related to Listeria monocytogenes Strains Isolated from Spontaneous Human Abortions in Tehran

Rezaei

Kazemi-Pour

Vandyousefi³

et al. 2019

Iran J Med Microbiol

View full text Add to dashboard Cite

Article Subject: Molecular Microbiology DOI: Background and Aims: Listeria monocytogenes, a gram positive, facultative, intracellular bacterium is the causative agent of listeriosis that is transmitted to human through raw and ready-to-eat foods. The aim of the present study was to determine dominant serovars of L. monocytogenes isolated from spontaneous abortion using phenotypic and genotypic methods. Materials and Methods: In present study, 258 clinical specimens including placental secretions, vaginal swabs and blood samples from 123 patients with abortion were selected in sterile condition then bacteriological, serological and molecular tests were conducted; dominant serotypes were identified by Multiplex PCR. Results: Out of 28 (%18.8) isolates of L. monocytogenes 21 (%17.7), 5 (%5.7) and 2 (%3.37) were isolated from placental secretions, vaginal swabs and blood respectively. Maximum and minimum isolated of bacteria related to placental secretions and vaginal swabs with 21 and 2 isolates respectively, of which 14 (%50) 1/2a, 10 (%35.7) 4b and 4 (%14.6) 2c serovars were reported for the first time. All of serovars played a key role in the spontaneous abortion as dominant and common serotypes in Iran. All of the isolates 28 (%22.76) showed hlyA gene and 24 isolates (%19.57) were positive for iap gene and compaired with control group there was significant different between the two groups (P<0.0002). Conclusion: The present study showed the isolation dominant and common serotypes of L. monocytogenes from spontaneous abortion and demonstrated that the presence of hlyA and iap were effective genes in increasing aggressive and pathogenicity. Serotypes that lacked the iap gene have less pathogenicity and influenced the pathogenesis in mice. It was also concluded that in the absence of access to molecular tests, performing PI-PLC, Congored and in vivo pathogenicity can be effective in detecting pathogenic serotypes from non-pathogenic L. monocytogenes.

show abstract

Distribution and Characterization of Dominant Serovars of Listeria Monocytogenes Strains Isolated from Spontaneous Human Abortion in Tehran

Cited by 3 publications

References 0 publications

Multiple drug resistance of Listeria monocytogenes isolated from aborted women by using serological and molecular techniques in Diwaniyah city/ Iraq

Multiple drug resistance of Listeria monocytogenes isolated from aborted women by using serological and molecular techniques in Diwaniyah city/ Iraq

Extraintestinal infection of Listeria monocytogenes and susceptibility to spontaneous abortion during pregnancy: A systematic review and meta-analysis

Determination of Dominant Serovars and Molecular Analysis of hly and iap Genes Related to Listeria monocytogenes Strains Isolated from Spontaneous Human Abortions in Tehran

Contact Info

Product

Resources

About