Distribution and characterization of class 1 integrons in Salmonella enterica serotype Gallinarum biotype Gallinarum

Kwon, Hanjung; Kim, Tae Eun; Cho, Sun Hee; Seol, Jae Goo; Kim, Bum Joon; Hyun, Jin Won; Park, Kyoung Yoon; Kim, Sun Joong; Yoo, Han Sang

doi:10.1016/s0378-1135(02)00257-2

Cited by 26 publications

(16 citation statements)

References 28 publications

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“…Interestingly, streptomycin/spectinomycin resistance gene cassettes (aadA1 and aadA2) were found to be the most frequent in the variable region of class 1 integrons ( Table 2). The similar predominant pattern of this aadA gene in class 1 integron has been reported in microbes from other species, suggesting that this gene may be either the first cassette to be acquired by an integron and/or may be more stably integrated into the integron than other gene cassettes [2,14].…”

Section: Determination Of the Phage Typesupporting

confidence: 74%

Assessment of Antibiotic Resistance Phenotype and Integrons in Salmonella enterica serovar Typhimurium Isolated from Swine

Rayamajhi

Kang

et al. 2008

The Journal of Veterinary Medical Science

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ABSTRACT. Salmonella enterica serovar Typhimurium (S. Typhimurium) isolated and identified from swine were subjected for the analysis of antibiotic resistance pattern and clinically important class 1 and 2 integrons. In addition, S. Typhimurium isolates exhibiting ampicillin, chloramphenicol, streptomycin, sulfamethoxazole, tetracycline and florfenicol (ACSSuTF) resistance pattern as described in most Salmonella enterica serotype Typhimurium definitive type 104 (DT104) were characterized by polymerase chain reaction. All the isolates were resistant to more than four antibiotics and showed the highest resistance to streptomycin (94.1%), followed by tetracycline (90.1%), ampicillin (64.7%), chloramphenicol (56.8%) and gentamicin (54.9%). MIC value for the ten isolates ranged between 0.125-2 µg/ml for ciprofloxacin. Among the beta-lactams used, only one of the isolate exhibited resistance to ceftiofur (MIC 8 µg/ml). Sixty eight percent of these multi drug resistance (MDR) S. Typhimurium isolates carried clinically important class 1 integron with 1kb (aadA) and/or 2kb (dhfrXII-orfF-aadA2) resistance gene cassettes. This study reports the increasing trend of multi drug resistance (MDR) S. Typhimurium with clinically important class 1 integron in pigs. In addition, emergence of the ACSSuTF-type resistance in S. Typhimurium PT other than DT104 may limit the use of resistance gene markers in its detection methods by PCR.

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Section: Determination Of the Phage Typesupporting

confidence: 74%

Assessment of Antibiotic Resistance Phenotype and Integrons in Salmonella enterica serovar Typhimurium Isolated from Swine

Rayamajhi

Kang

et al. 2008

The Journal of Veterinary Medical Science

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“…Class 1 integrons with a variety of gene cassettes conferring resistance to antimicrobials such as aminoglycosides (aadA1, aadA2, aacA4, and aadB), trimethoprim (dfrA1, dfrA7, and dfrA12), b-lactams (blaPSE1 and blaOXA30), and chloramphenicol (cmlA and cmlA5) have been reported in MDR S. enterica isolates; clonal expansion and horizontal gene transfer may have contributed to the spread of antimicrobial resistance integrons in these organisms (Levesque et al, 1995;Krauland et al, 2009). In a previous study, examination of S. enterica serovar Gallinarum isolates acquired between 1992 and 2001 in South Korea most frequently detected class 1 integrons containing one (aadA), two (aadB-aadA), or three resistance genes (dhfrXII-orfF-aadA) and the integron containing the aadA gene cassette (91%) (Kwon et al, 2002). Although new resistance genes other than aminoglycoside resistance genes were not evident in the integrons of the S. enterica serovar Gallinarum isolates acquired since 2002, the frequency of integrons containing two resistance genes, aadB-aadA, has increased since 2002.…”

Section: Discussionmentioning

confidence: 95%

Characterization of antimicrobial resistance of recentSalmonella entericaserovar Gallinarum isolates from chickens in South Korea

et al. 2010

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Salmonella enterica serovar Gallinarum isolates (n 0105) from chickens in South Korea between 2002 and 2007 were tested for antimicrobial susceptibility by determining minimum inhibitory concentrations of 16 antimicrobials, and their predominant resistance profiles were genetically characterized. Most isolates (99/ 105; 94.3%) were resistant to nalidixic acid and resistant/intermediately resistant to fluoroquinolones, and 63.8% (67/105) of the isolates were resistant to three or more antimicrobials. Forty-two quinolone-resistant isolates, of which the quinolone resistance-determining regions of the gyrA genes were sequenced, contained a substitution of a Ser to a Phe or Tyr at position 83 (71.4%), or a substitution of an Asp to an Asn, Gly, or Tyr at position 87 (28.6%). Fifty-seven sulphamethoxazole-resistant isolates were tested for the presence of class 1 integrons by polymerase chain reaction, and their resistance gene cassettes were analysed by sequencing. Three different class 1 integrons containing the resistance-gene insert aadA (52.6%; n 030), aadB (12.3%; n07), or aadB-aadA (12.3%; n 07) were identified. Most isolates harbouring the integron containing aadB-aadA displayed resistance to all three aminoglycosides tested and also showed increased resistance to fluoroquinolones. These findings suggest that fluoroquinolone resistance may be epidemiologically linked to multiple aminoglycoside resistance.

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“…All isolates were evaluated for the presence of class 1 integrons using the 5ЈCS-3ЈCS primer set (23). PCR was conducted as described previously (20).…”

Section: Bacteriamentioning

confidence: 99%

Chronological Study of Antibiotic Resistances and Their Relevant Genes in Korean Avian Pathogenic Escherichia coli Isolates

Kim¹,

Jeong²,

Cho

et al. 2007

J Clin Microbiol

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Antibiograms and relevant genotypes of Korean avian pathogenic Escherichia coli (APEC) isolates (n ‫؍‬ 101) recovered between 1985 and 2005 were assessed via disc diffusion test, PCR, restriction enzyme analysis, and sequencing. These isolates were highly resistant to tetracycline (84.2%), streptomycin (84.2%), enrofloxacin (71.3%), and ampicillin (67.3%), and most of the tetracycline, streptomycin, enrofloxacin, and ampicillin resistances were associated with tetA and/or tetB, aadA and/or strA-strB, mutations in gyrA and/or parC, and TEM, respectively. Class 1 integrons were detected in 40 isolates (39.6%), and a variety of gene cassettes conferring streptomycin (aadA), gentamicin (aadB), and trimethoprim (dfr) resistances were identified: aadA1a (27.5%), dfrV-orfD (2.5%), aadB-aadA1a (2.5%), dfrI-aadA1a (47.5%), dfrXVII-aadA5 (12.5%), and dfrXII-orfFaadA2 (7.5%). In addition, several types of common promoters (P ant ) of the gene cassettes (hybrid P1, weak P1, or weak P1 plus P2) and single-nucleotide polymorphisms in aadA1a were identified. The results of a chronological analysis demonstrated significant and continuous increases in the frequencies of resistances to several antibiotics (tetracycline, streptomycin, enrofloxacin, ampicillin, and trimethoprim-sulfamethoxazole) and of the relevant resistance genes (tetA, strA-strB, and TEM), mutations in gyrA and parC, and multidrugresistant APEC strains during the period 2000 to 2005.

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Distribution and characterization of class 1 integrons in Salmonella enterica serotype Gallinarum biotype Gallinarum

Cited by 26 publications

References 28 publications

Assessment of Antibiotic Resistance Phenotype and Integrons in Salmonella enterica serovar Typhimurium Isolated from Swine

Assessment of Antibiotic Resistance Phenotype and Integrons in Salmonella enterica serovar Typhimurium Isolated from Swine

Characterization of antimicrobial resistance of recentSalmonella entericaserovar Gallinarum isolates from chickens in South Korea

Chronological Study of Antibiotic Resistances and Their Relevant Genes in Korean Avian Pathogenic Escherichia coli Isolates

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