Distribution and change patterns of free IAA, ABP 1 and PM H+-ATPase during ovary and ovule development of Nicotiana tabacum L.

Chen, Dan; Deng, Yadong; Jie, Zhao

doi:10.1016/j.jplph.2011.08.017

Cited by 8 publications

(4 citation statements)

References 65 publications

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“…Contrary to these findings, our study indicated that a predominant fraction, exceeding 70%, of the SaSAUR proteins was predicted to be localized within the chloroplasts, a pattern similarly observed in maize [69]. Plant chloroplasts are important intracellular organelles that produce ATP (Adenosine triphosphate) through photosynthetic phosphorylation, suggesting that auxins may play a role in the energy transfer process that takes place at the chloroplast membrane [70].…”

Section: Discussioncontrasting

confidence: 82%

Genome-Wide Analysis of the SAUR Gene Family and Its Expression Profiles in Response to Salt Stress in Santalum album

Zhu,

Zheng,

et al. 2024

Plants

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The SAUR (small auxin-up RNA) family constitutes a category of genes that promptly respond to the hormone auxin and play a pivotal role in diverse biological processes encompassing plant growth and the response to abiotic stress. Santalum album L., a semi-parasitic evergreen tree, is renowned for its economically valuable essential oils, positioning it among the most prized tree species. In this study, a meticulous identification and comprehensive analysis of 43 SAUR genes was conducted within S. album. Based on phylogenetic relationships, the SaSAUR genes were systematically categorized into five groups. A collinearity analysis revealed intriguing insights, disclosing 14 segmental duplications and 9 tandem duplications within the SaSAUR genes, emphasizing the pivotal role of duplication in the expansion of this gene family. Noteworthy variations in the expression levels of SaSAUR genes were observed by delving into the SaSAUR transcriptome data from various tissues, including leaves, roots, and heartwood, as well as under salt-stress conditions. Notably, SaSAUR08 and SaSAUR13 were significantly upregulated in heartwood compared with roots and leaves, while SaSAUR18 was markedly more expressed in roots compared with heartwood and leaves. Furthermore, SaSAUR27 and SaSAUR28 were found to respond closely to salt stress, hinting at their potential involvement in the salt-stress response mechanism. This research offers a comprehensive investigation of SAUR genes in S. album and establishes a foundation for future exploration of the SAUR gene family, particularly its relation to growth and salt-stress responses.

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Section: Discussioncontrasting

confidence: 82%

Genome-Wide Analysis of the SAUR Gene Family and Its Expression Profiles in Response to Salt Stress in Santalum album

Zhu,

Zheng,

et al. 2024

Plants

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“…At the level of plant organs, ABP1 is expressed primarily in meristems. However, it can be found throughout the whole plant body of all land plants tested [11], and recently ABP1 was localized in tobacco in ovary, egg cells and in embryos at all developmental stages [12,13]. …”

Section: Introductionmentioning

confidence: 99%

Overexpression of the Auxin Binding PROTEIN1 Modulates PIN-Dependent Auxin Transport in Tobacco Cells

et al. 2013

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BackgroundAuxin binding protein 1 (ABP1) is a putative auxin receptor and its function is indispensable for plant growth and development. ABP1 has been shown to be involved in auxin-dependent regulation of cell division and expansion, in plasma-membrane-related processes such as changes in transmembrane potential, and in the regulation of clathrin-dependent endocytosis. However, the ABP1-regulated downstream pathway remains elusive.Methodology/Principal FindingsUsing auxin transport assays and quantitative analysis of cellular morphology we show that ABP1 regulates auxin efflux from tobacco BY-2 cells. The overexpression of ABP1can counterbalance increased auxin efflux and auxin starvation phenotypes caused by the overexpression of PIN auxin efflux carrier. Relevant mechanism involves the ABP1-controlled vesicle trafficking processes, including positive regulation of endocytosis of PIN auxin efflux carriers, as indicated by fluorescence recovery after photobleaching (FRAP) and pharmacological manipulations.Conclusions/SignificanceThe findings indicate the involvement of ABP1 in control of rate of auxin transport across plasma membrane emphasizing the role of ABP1 in regulation of PIN activity at the plasma membrane, and highlighting the relevance of ABP1 for the formation of developmentally important, PIN-dependent auxin gradients.

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“…Hand pollination using mature pollen can be performed on immature flowers ( Haring et al, 1990 ) in an attempt to bypass the effects of self-incompatibility on species of Brassicaceae ( Hiscock and Dickinson, 1993 ), as well as Solanaceae species ( Chalivendra et al, 2013 ). In Nicotiana tabacum , a species with a Polygonum-type embryo sac ( Huang and Russell, 1992 ), the ovule is not fully developed at anthesis, and the egg cell is not usually observed at the embryo sacs ( Tian and Russell, 1997 ; Lobanova and Enaleeva, 1998 ; De Martinis and Mariani, 1999 ; Chen et al, 2012 ). In this species, the effects of pollination on ovule development have mainly been examined at stages close to anthesis.…”

Section: Introductionmentioning

confidence: 99%

Pollination triggers female gametophyte development in immature Nicotiana tabacum flowers

et al. 2015

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In Nicotiana tabacum, female gametophytes are not fully developed at anthesis, but flower buds pollinated 12 h before anthesis produce mature embryo sacs. We investigated several pollination-associated parameters in N. tabacum flower buds to determine the developmental timing of important events in preparation for successful fertilization. First, we performed hand pollinations in flowers from stages 4 to 11 to study at which developmental stage pollination would produce fruits. A Peroxtesmo test was performed to correlate peroxidase activity on the stigma surface, indicative of stigma receptivity, with fruit set. Pollen tube growth and female gametophyte development were microscopically analyzed in pistils of different developmental stages. Fruits were obtained only after pollinations of flower buds at late stage 7 and older; fruit weight and seed germination capacity increased as the developmental stage of the pollinated flower approached anthesis. Despite positive peroxidase activity and pollen tube growth, pistils at stages 5 and 6 were unable to produce fruits. At late stage 7, female gametophytes were undergoing first mitotic division. After 24 h, female gametophytes of unpollinated pistils were still in the end of the first division, whereas those of pollinated pistils showed egg cells. RT-qPCR assay showed that the expression of the NtEC1 gene, a marker of egg cell development, is considerably higher in pollinated late stage 7 ovaries compared with unpollinated ovaries. To test whether ethylene is the signal eliciting female gametophyte maturation, the expression of ACC synthase was examined in unpollinated and pollinated stage 6 and late stage 7 stigmas/styles. Pollination induced NtACS expression in stage 6 pistils, which are unable to produce fruits. Our results show that pollination is a stimulus capable of triggering female gametophyte development in immature tobacco flowers and suggests the existence of a yet undefined signal sensed by the pistil.

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Distribution and change patterns of free IAA, ABP 1 and PM H+-ATPase during ovary and ovule development of Nicotiana tabacum L.

Cited by 8 publications

References 65 publications

Genome-Wide Analysis of the SAUR Gene Family and Its Expression Profiles in Response to Salt Stress in Santalum album

Genome-Wide Analysis of the SAUR Gene Family and Its Expression Profiles in Response to Salt Stress in Santalum album

Overexpression of the Auxin Binding PROTEIN1 Modulates PIN-Dependent Auxin Transport in Tobacco Cells

Pollination triggers female gametophyte development in immature Nicotiana tabacum flowers

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