Distribution And Antifungal Susceptibility Of Candida Species Isolated From Blood Cultures

Kılınçel, Özge; Akar, Nida; Karamurat, Zeynep Dilara; Çalışkan, Emel; Öksüz, Şükrü; Öztürk, Cihadiye Elif; Şahi̇n, İdris

doi:10.5222/tmcd.2018.256

Cited by 5 publications

(3 citation statements)

References 22 publications

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“…Among non-albicans Candida spp., C. glabrata SC is the most commonly isolated species in North America, Northern China, and Northern Europe, while C. parapsilosis SC ranks first in Southern Europe and Latin America [24][25]. In studies conducted in our country, C. parapsilosis SC has been reported as the most common species among non-albicans species with different rates (6-66%) [11,[26][27][28][29][30][31][32]. Consistent with this, C. parapsilosis SC (16.9%) was the second most frequently isolated species in our center, following C. albicans.…”

Section: Discussionmentioning

confidence: 99%

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Species Distribution and Antifungal Susceptibilities of Candida Species Isolated From Blood Culture

Cilo¹

2023

Cureus

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Introduction Candida species (spp.) are among the leading agents of bloodstream infections. Candidemias are a major cause of morbidity and mortality. Having an understanding of Candida epidemiology and antifungal susceptibility patterns in each center is crucial in guiding the management of candidemia. In this study, the species distribution and antifungal susceptibility of Candida spp. isolated from blood culture at the University of Health Sciences, Bursa Yuksek Ihtisas Training & Research Hospital were examined and the first data on the epidemiology of candidemia in our center were presented. Methods A total of 236 Candida strains isolated from blood cultures in our hospital over a four-year period were analyzed and their antifungal susceptibilities were studied retrospectively. Strains were identified at the species complex (SC) level by the germ tube test, morphology in cornmeal-tween 80 medium, and the automated VITEK 2 Compact (bioMérieux, Marcy-l'Étoile, France) system. Antifungal susceptibility tests were performed on VITEK 2 Compact (bioMérieux, Marcy-l'Étoile, France) system. The susceptibilities of the strains to fluconazole, voriconazole, micafungin, and amphotericin B were determined according to Clinical and Laboratory Standards Institute (CLSI) guidelines and epidemiologic cut-off values. Results Of the Candida (C.) strains, 131 were C. albicans (55.5%), 40 were C. parapsilosis SC (16.9%), 21 were C. tropicalis (8.9%), 19 were C. glabrata SC (8.1%), eight were C. lusitaniae (3.4%), seven were C. kefyr (3.0%), six were C. krusei (2.6%), two were C. guilliermondii (0.8%) and two were C. dubliniensis (0.8%). Amphotericin B resistance was not detected in Candida strains. Micafungin susceptibility was 98.3%, and four C. parapsilosis SC strains (10%) were intermediate (I) to micafungin. Fluconazole susceptibility was 87.2%. Apart from C. krusei strains which intrinsically resistant to fluconazole, three C. parapsilosis (7.5%), one C. glabrata SC (5.3%) strain were resistant (R) to fluconazole, and one C. lusitaniae (12.5%) strain was wild-type (WT). Voriconazole susceptibility of Candida strains was 98.6%. Two C. parapsilosis SC strains were I to voriconazole, while one strain was R. Conclusion In this study, the first epidemiological data of candidemia agents in our hospital were presented. It was determined that rare and naturally resistant species did not cause any problem in our center yet. C. parapsilosis ...

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Section: Discussionmentioning

confidence: 99%

“…In studies from different centers, C. tropicalis or C. glabrata SC ranks second among non-albicans species [11,[26][27][28][29][30][31][32]. In recent years, infections caused by C. glabrata SC have been increasing [33].…”

Section: Discussionmentioning

confidence: 99%

Species Distribution and Antifungal Susceptibilities of Candida Species Isolated From Blood Culture

Cilo¹

2023

Cureus

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“…Dünya genelinde yapılan birçok tek veya çok merkezli kandidemi çalışmalarında, C. parapsilosis tür kompleksinin C. albicans'dan sonra ikinci sırada görüldüğü bildirilmektedir (2,3,(23)(24)(25) . Ülkemizde yapılan çalışmalarda da pek çok merkezde C. albicans'dan sonra en fazla C. parapsilosis tür kompleksine rastlanılmaktadır (5)(6)(7)(8)19,(26)(27)(28)(29) .…”

Section: Bulgularunclassified

Identification of Candida parapsilosis Complex Strains Isolated from Blood Samples at Species Level and Determination of Their Antifungal Susceptibilities

Cilo¹,

Ağca²,

Ener³

2019

TMCD

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ÖZ Amaç: Dünyada ve ülkemizde birçok merkezde, fırsatçı bir enfeksiyon olan kandidemilere ikinci sıklıkta neden olan tür Candida parapsilosis tür kompleksidir. Bu tür kompleksi içinde yer alan Candida parapsilosis sensu stricto, Candida orthopsilosis ve Candida metapsilosis türleri fenotipik olarak birbirinden ayrılamaz ve tanımlanmaları için çeşitli moleküler yöntemlere gereksinim duyulur. Bu çalışmada, laboratuvarımızda kan kültürlerinden izole edilen C. parapsilosis tür kompleksi suşlarının antifungal duyarlılıklarının incelenmesi ve tür düzeyinde tanımlama yapılması amaçlanmıştır. Yöntem: Uludağ Üniversitesi Tıp Fakültesi Tıbbi Mikrobiyoloji Anabilim Dalı stoklarından 118 kan izolatı kullanıldı. İzolatların flukonazol, vorikonazol, itrakonazol, posakonazol, amfoterisin B ve anidulafungine in vitro duyarlılığı CLSI-M27 dokümanına göre saptandı. C. parapsilosis tür kompleksindeki türler, sekonder alkol dehidrogenaz enzimini kodlayan gen bölgesinin (SADH) "polimeraz chain reaction-restriction fragment length polymorphism" (PCR-RFLP) yöntemiyle incelenmesiyle belirlendi. Bulgular: Bu çalışmada, kan kültürlerinden izole edilen 118 C. parapsilosis tür kompleksi suşunda flukonazol direncinin %26.3 oranında olduğu, amfoterisin B ve anidulafunginde ise direncin yok denecek kadar az olduğu saptandı. Antifungal duyarlılığı belirlenen bu suşlar arasında C. orthopsilosis ve C. metapsilosis türlerine rastlanmadı. Sonuç: Bu çalışma ile merkezimizdeki C. parapsilosis tür kompleksindeki yüksek flukonazol direncinin kompleks içindeki türlerle ilişkili olmadığı görüldü. Suşlar arasındaki klonal ilişkinin ve direncin genetik temellerinin araştırılması için ek çalışmaların yapılması gerektiği sonucuna varıldı. Anahtar kelimeler: Candida parapsilosis tür kompleksi, antifungal duyarlılık, PCR-RFLPABSTRACT Objective: Candida parapsilosis species complex is the second most common cause of candidiasis which is an opportunistic infection, in many centers in the world and in our country. Candida parapsilosis sensu stricto, Candida orthopsilosis and Candida metapsilosis within this species complex are indistinguishable from each other phenotypically and various molecular methods are needed for their identification. In this study, we aimed to investigate the antifungal susceptibility of C. parapsilosis species complex isolated from blood cultures in our laboratory and to identify them at species level. Method: A total of 118 blood isolates from the stock culture of Uludağ University Faculty of Medicine Department of Microbiology were used. The in vitro sensitivity of the isolates to fluconazole, voriconazole, itraconazole, posaconazole, amphotericin B and anidulafungin was determined according to the CLSI-M27 document. The species in the C. parapsilosis species complex were determined by the investigation of the gene region encoding the secondary alcohol dehydrogenase enzyme (SADH) using "polymerase chain reaction-restriction fragment length polymorphism" (PCR-RFLP) method. Results: In this study, fluconazole resistance was found to be 26...

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Candida parapsilosis candidemia in children admitted to a tertiary hospital in Turkey: clinical features and antifungal susceptibility

Çetin,

Çay,

Polat

et al. 2024

Microbiol Spectr

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In recent years, the incidence and drug resistance of Candida parapsilosis have increased. Our study aimed to determine the antifungal sensitivity of C. parapsilosis and the clinical and demographic characteristics of children with candidemia. Two hundred pediatric patients with C. parapsilosis candidemia were included in the study between 1 January 2010 and 1 August 2023. Clinical samples were evaluated on a BACTEC-FX-40 automatic blood culture device (Becton Dickinson, USA). Yeast isolates were identified to the species level via identification cards (YST) using the VITEK 2 Compact (bioMeriéux, France) system. Antifungal susceptibility was performed using antifungal cell cards (AST-YST01). Approval for the study was received from the “University Faculty of Medicine” Hospital Clinical Research Ethics Committee. Non-catheter candidemia was detected in 127 (63.5%) patients, and catheter-related candidemia was detected in 73 (36.5%) patients. It was observed that the patients’ history of malignancy, mechanical ventilation, urinary catheter, nasogastric tube, and intensive care unit stay was associated with C. parapsilosis mortality. The mortality rate from candidemia was 9.5%. The most frequently preferred antifungal agents were amphotericin B and fluconazole. The fluconazole drug resistance rate was found to be 6%, and the amphotericin B drug resistance rate was 4%. Because C. parapsilosis candidemia mortality rates can be high depending on risk factors and clinical characteristics, it is important to initiate appropriate and timely antifungal therapy. We think that our study can provide important information about the clinical profiles, distributions, susceptibility profiles, and control of antifungal resistance of C. parapsilosis isolates. IMPORTANCE It has been observed that the frequency and antifungal resistance of Candida parapsilosis have increased recently. In our study, we aimed to determine the antifungal sensitivity of C. parapsilosis and the clinical and demographic characteristics of children with candidemia. It was observed that the patients’ history of malignancy, mechanical ventilation, urinary catheter, nasogastric tube, and intensive care stay was associated with C. parapsilosis mortality. The mortality rate from candidemia was 9.5%. The most frequently preferred antifungal agents were amphotericin B and fluconazole. The fluconazole drug resistance rate was found to be 6%, and the amphotericin B drug resistance rate was 4%. Because C. parapsilosis candidemia mortality rates can be high depending on risk factors and clinical characteristics, it is important to initiate appropriate and timely antifungal therapy.

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Distribution And Antifungal Susceptibility Of Candida Species Isolated From Blood Cultures

Cited by 5 publications

References 22 publications

Species Distribution and Antifungal Susceptibilities of Candida Species Isolated From Blood Culture

Species Distribution and Antifungal Susceptibilities of Candida Species Isolated From Blood Culture

Identification of Candida parapsilosis Complex Strains Isolated from Blood Samples at Species Level and Determination of Their Antifungal Susceptibilities

Candida parapsilosis candidemia in children admitted to a tertiary hospital in Turkey: clinical features and antifungal susceptibility

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