Distribution and activity of microorganisms in the deep repository for liquid radioactive waste at the Siberian Chemical Combine

Назина, Т. Н.; Luk’yanova, E. A.; Zakharova, Еlena V.; Ivoilov, V. S.; Полтараус, А. Б.; Kalmykov, Stepan N.; Belyaev, S. S.; Zubkov, A. A.

doi:10.1134/s0026261706060178

Cited by 7 publications

(5 citation statements)

References 14 publications

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“…We found that Gammaproteobacterium HK31.3 associated with the wells HK31 (see Gislason et al, 2010 for description) and P. reactans strain SR3 (Genbank ID GQ258636), share 100% identity over 927 bases at the level of their 16S RNA genes (GenBank accession number BankIt1496454 HK31.3 JQ071900). Pseudomonas strains is a common groundwater microbe frequently observed in repository environments (e.g., Nazina et al, 2006Nazina et al, , 2010. For most experiments, cells were first cultured to a stationary stage on a shaker at 25°C in the darkness using final 10% NB with the following composition: 0.1 g/L glucose, 1.5 g/L peptone, 0.6 g/L NaCl and 0.3 g/L yeast extract.…”

Section: Bacteria Culturesmentioning

confidence: 99%

Effect of the heterotrophic bacterium Pseudomonas reactans on olivine dissolution kinetics and implications for CO2 storage in basalts

Shirokova

Bénézeth

Pokrovsky

et al. 2012

Geochimica et Cosmochimica Acta

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Section: Bacteria Culturesmentioning

confidence: 99%

Effect of the heterotrophic bacterium Pseudomonas reactans on olivine dissolution kinetics and implications for CO2 storage in basalts

Shirokova

Bénézeth

Pokrovsky

et al. 2012

Geochimica et Cosmochimica Acta

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“…With regard to the genera Kocuria and Micrococcus , which, in the present study, also displayed good performance in the uptake and decontamination of uranium, the species Kocuria erythromyxa was able to adsorb 68% of uranium at 0.1 µM [ 54 , 55 ]. The species M. luteus has been shown to bind up to 2.6 mmol uranium/g dry weight [ 56 ].…”

Section: Resultsmentioning

confidence: 95%

First Isolation and Characterization of Bacteria from the Core’s Cooling Pool of an Operating Nuclear Reactor

et al. 2023

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Microbial life can thrive in the most inhospitable places, such as nuclear facilities with high levels of ionizing radiation. Using direct meta-analyses, we have previously highlighted the presence of bacteria belonging to twenty-five different genera in the highly radioactive water of the cooling pool of an operating nuclear reactor core. In the present study, we further characterize this specific environment by isolating and identifying some of these microorganisms and assessing their radiotolerance and their ability to decontaminate uranium. This metal is one of the major radioactive contaminants of anthropogenic origin in the environment due to the nuclear and mining industries and agricultural practices. The microorganisms isolated when sampling was performed during the reactor operation consisted mainly of Actinobacteria and Firmicutes, whereas Proteobacteria were dominant when sampling was performed during the reactor shutdown. We investigated their tolerance to gamma radiation under different conditions. Most of the bacterial strains studied were able to survive 200 Gy irradiation. Some were even able to withstand 1 kGy, with four of them showing more than 10% survival at this dose. We also assessed their uranium uptake capacity. Seven strains were able to remove almost all the uranium from a 5 µM solution. Four strains displayed high efficiency in decontaminating a 50 µM uranium solution, demonstrating promising potential for use in bioremediation processes in environments contaminated by radionuclides.

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“…This, combined with metagenomics data, led the authors to hypothesize that these taxa are responsible for the consumption of hydrogen and obtain their energy by coupling hydrogen oxidation to the reduction of a range of electron acceptors. Although cyanobacteria have previously been reported in various deep environments, their abundance has been difficult to explain [51,52,53,54]. This latest discovery at Rio Tinto suggests that these non-photosynthesizing Cyanobacteria might be active members of the microbial community, rather than inactive cells that were washed down and trapped in the subsurface or arising from contamination during sampling, as previously postulated.…”

Section: Microbial Hydrogen Consumptionmentioning

confidence: 96%

Subsurface Microbial Hydrogen Cycling: Natural Occurrence and Implications for Industry

et al. 2019

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Hydrogen is a key energy source for subsurface microbial processes, particularly in subsurface environments with limited alternative electron donors, and environments that are not well connected to the surface. In addition to consumption of hydrogen, microbial processes such as fermentation and nitrogen fixation produce hydrogen. Hydrogen is also produced by a number of abiotic processes including radiolysis, serpentinization, graphitization, and cataclasis of silicate minerals. Both biotic and abiotically generated hydrogen may become available for consumption by microorganisms, but biotic production and consumption are usually tightly coupled. Understanding the microbiology of hydrogen cycling is relevant to subsurface engineered environments where hydrogen-cycling microorganisms are implicated in gas consumption and production and corrosion in a number of industries including carbon capture and storage, energy gas storage, and radioactive waste disposal. The same hydrogen-cycling microorganisms and processes are important in natural sites with elevated hydrogen and can provide insights into early life on Earth and life on other planets. This review draws together what is known about microbiology in natural environments with elevated hydrogen, and highlights where similar microbial populations could be of relevance to subsurface industry.

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Distribution and activity of microorganisms in the deep repository for liquid radioactive waste at the Siberian Chemical Combine

Cited by 7 publications

References 14 publications

Effect of the heterotrophic bacterium Pseudomonas reactans on olivine dissolution kinetics and implications for CO2 storage in basalts

Effect of the heterotrophic bacterium Pseudomonas reactans on olivine dissolution kinetics and implications for CO2 storage in basalts

First Isolation and Characterization of Bacteria from the Core’s Cooling Pool of an Operating Nuclear Reactor

Subsurface Microbial Hydrogen Cycling: Natural Occurrence and Implications for Industry

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