Distribution and Abundance of Archaea in South China Sea Sponge <i>Holoxea</i> sp. and the Presence of Ammonia‐Oxidizing Archaea in Sponge Cells

Liu, Fang; Han, Minqi; Zhang, Fengli; Zhang, Baohua; Li, Zhiyong

doi:10.1155/2011/723696

Cited by 9 publications

(10 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Symbionts in sponges have been found to exist both intracellularly and extracellularly (Liu et al . ; Hentschel et al . ), and in both cases, phagocytic processes have to be modified to allow for this.…”

Section: Discussionmentioning

confidence: 99%

Evolution and function of eukaryotic‐like proteins from sponge symbionts

Reynolds

Thomas

2016

Molecular Ecology

View full text Add to dashboard Cite

Sponges (Porifera) are ancient metazoans that harbour diverse microorganisms, whose symbiotic interactions are essential for the host's health and function. Although symbiosis between bacteria and sponges are ubiquitous, the molecular mechanisms that control these associations are largely unknown. Recent (meta-) genomic analyses discovered an abundance of genes encoding for eukaryotic-like proteins (ELPs) in bacterial symbionts from different sponge species. ELPs belonging to the ankyrin repeat (AR) class from a bacterial symbiont of the sponge Cymbastela concentrica were subsequently found to modulate amoebal phagocytosis. This might be a molecular mechanism, by which symbionts can control their interaction with the sponge. In this study, we investigated the evolution and function of ELPs from other classes and from symbionts found in other sponges to better understand the importance of ELPs for bacteria-eukaryote interactions. Phylogenetic analyses showed that all of the nine ELPs investigated were most closely related to proteins found either in eukaryotes or in bacteria that can live in association with eukaryotes. ELPs were then recombinantly expressed in Escherichia coli and exposed to the amoeba Acanthamoeba castellanii, which is functionally analogous to phagocytic cells in sponges. Phagocytosis assays with E. coli containing three ELP classes (AR, TPR-SEL1 and NHL) showed a significantly higher percentage of amoeba containing bacteria and average number of intracellular bacteria per amoeba when compared to negative controls. The result that various classes of ELPs found in symbionts of different sponges can modulate phagocytosis indicates that they have a broader function in mediating bacteria-sponge interactions.

show abstract

Section: Discussionmentioning

confidence: 99%

Evolution and function of eukaryotic‐like proteins from sponge symbionts

Reynolds

Thomas

2016

Molecular Ecology

View full text Add to dashboard Cite

show abstract

“…Our results are in agreement with previous studies detecting up to 5.5 9 10 7 16S rRNA gene copies per microgram DNA in the mesohyl of Holoxea sp. (Liu et al, 2011). Archaea, represented by few specific clades related to Cenarcharchaeum symbiosum (phylum Thaumarchaeota), are frequently found in sponges, in some cases at very high abundances (Preston et al, 1996;Pape et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Quantification of bacterial and archaeal symbionts in high and low microbial abundance sponges using real-time PCR

Bayer

Kamke

Hentschel

2014

FEMS Microbiol Ecol

View full text Add to dashboard Cite

In spite of considerable insights into the microbial diversity of marine sponges, quantitative information on microbial abundances and community composition remains scarce. Here, we established qPCR assays for the specific quantification of four bacterial phyla of representative sponge symbionts as well as the kingdoms Eubacteria and Archaea. We could show that the 16S rRNA gene numbers of Archaea, Chloroflexi, and the candidate phylum Poribacteria were 4-6 orders of magnitude higher in high microbial abundance (HMA) than in low microbial abundance (LMA) sponges and that actinobacterial 16S rRNA gene numbers were 1-2 orders higher in HMA over LMA sponges, while those for Cyanobacteria were stable between HMA and LMA sponges. Fluorescence in situ hybridization of Aplysina aerophoba tissue sections confirmed the numerical dominance of Chloroflexi, which was followed by Poribacteria. Archaeal and actinobacterial cells were detected in much lower numbers. By use of fluorescence-activated cell sorting as a primer- and probe-independent approach, the dominance of Chloroflexi, Proteobacteria, and Poribacteria in A. aerophoba was confirmed. Our study provides new quantitative insights into the microbiology of sponges and contributes to a better understanding of the HMA/LMA dichotomy.

show abstract

“…Archaeal amoA sequences were distinct from those found in other sponges (Bayer et al 2008, Meyer & Kuever 2008, Steger et al 2008, Hoffmann et al 2009, López-Legentil et al 2010, Turque et al 2010, Liu et al 2011, Han et al 2012, Radax et al 2012, Ribes et al 2012. The closest relative to sequences retrieved from the tropical sponge H. caerulea was from the coral Diploria strigosa (Beman et al 2007), while amoA se quences from water surrounding the sponge were similar to sequences retrieved from the warmwater sponge Siphonochalina sp.…”

Section: Diversity Of Sponge-associated Aoa and Aobmentioning

confidence: 83%

“…Ammonia-oxidizing Archaea (AOA) have also been receiving increasing attention since the discovery of the ammonia-oxidizing thaumarchaeote Cenarchaeum symbiosum associated with the sponge Axinella mexicana (Preston et al 1996). Thaumarchaeotes, in particular, appear capable of oxidizing ammonia (Bayer et al 2008, Turque et al 2010, Liu et al 2011, Pester et al 2011. Recently, the presence of AOB and AOA was described for a diverse range of sponges from the Pacific, Caribbean, Mediterranean and North At lantic (Bayer et al 2008, Meyer & Kuever 2008, Steger et al 2008, López-Legentil et al 2010, Mohamed et al 2010, Liu et al 2011, Radax et al 2012.…”

Section: Introductionmentioning

confidence: 99%

“…Thaumarchaeotes, in particular, appear capable of oxidizing ammonia (Bayer et al 2008, Turque et al 2010, Liu et al 2011, Pester et al 2011. Recently, the presence of AOB and AOA was described for a diverse range of sponges from the Pacific, Caribbean, Mediterranean and North At lantic (Bayer et al 2008, Meyer & Kuever 2008, Steger et al 2008, López-Legentil et al 2010, Mohamed et al 2010, Liu et al 2011, Radax et al 2012. Nitrification by sponges may well be significant in coral reef ecosystems, considering the high biomass of sponges in such systems (Diaz & Rützler 2001, Reitner & Hoffmann 2003, Van Soest et al 2007a).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Diversity and abundance of ammonia-oxidizing Archaea and Bacteria in tropical and cold-water coral reef sponges

Cardoso¹,

Bleijswijk²,

Witte³

et al. 2013

Aquat. Microb. Ecol.

View full text Add to dashboard Cite

We analysed the diversity and abundance of ammonia-oxidizing Archaea (AOA) and Bacteria (AOB) in the shallow warm-water sponge Halisarca caerulea and the deep cold-water sponges Higginsia thielei and Nodastrella nodastrella. The abundance of AOA and AOB was analysed using catalyzed reporter deposition-fluorescence in situ hybridization and (real-time) quantitative PCR (Q-PCR) targeting archaeal and bacterial amoA genes. Archaeal abundance was similar between sponge species, while bacterial abundance was higher in H. caerulea than in N. nodastrella and H. thielei. Q-PCR showed that AOA outnumbered AOB by a factor of 2 to 35, suggesting a larger role of AOA than of AOB in ammonia oxidation in sponges. PCR-denaturing gradient gel electrophoresis was performed to analyse the taxonomic affiliation of the microbial community associated with these sponges. Archaeal and bacterial amoA genes were found in all 3 sponges. The structure of the phylogenetic trees in relation to temperature and sponge species was analysed using all published amoA sequences retrieved from sponges. Temperature was an important factor influencing the distribution of nitrifiers in sponges. Both archaeal and bacterial amoA sponge sequences tended to cluster with sequences retrieved from habitats of similar temperature. This is the first time that similarity in AOB diversity is described between distantly related species (H. thielei belonging to the class Demospongiae, and N. nodastrella to Hexactinellida). The results described here support the idea of a relatively uniform microbial community between distantly related sponges and suggest that temperature (rather than phylogenetic distance) is determining the diversity of AOA and AOB in sponges.

show abstract

Distribution and Abundance of Archaea in South China Sea Sponge Holoxea sp. and the Presence of Ammonia‐Oxidizing Archaea in Sponge Cells

Cited by 9 publications

References 39 publications

Evolution and function of eukaryotic‐like proteins from sponge symbionts

Evolution and function of eukaryotic‐like proteins from sponge symbionts

Quantification of bacterial and archaeal symbionts in high and low microbial abundance sponges using real-time PCR

Diversity and abundance of ammonia-oxidizing Archaea and Bacteria in tropical and cold-water coral reef sponges

Contact Info

Product

Resources

About