Distribution, adaptation and physiological meaning of thiols from vertebrate hemoglobins

Reischl, Evaldo; Dafre, Alcir Luiz; Franco, Jeferson Luís; Filho, Danilo Wilhelm

doi:10.1016/j.cbpc.2006.07.015

Cited by 48 publications

(50 citation statements)

References 147 publications

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“…NPSH also shows the antioxidant capacity acting against the formation of free radicals, in the maintenance of the cell redox balance, as well as in the defense against electrophilic agents (Reischl et al, 2007). Our results demonstrate consistency between antioxidant analyses because both NPSH and GST showed higher levels in gill and liver of fish from dam.…”

Section: Discussionsupporting

confidence: 70%

“…An important parameter is the activity of glutathione-S-transferase (GST), catalyzing the conjugation of several xenobiotics with glutathione (GSH), protecting lipids from peroxidation during the detoxification process (Cairrão et al, 2004). The non-protein thiols (NPSH) also show antioxidant capacity acting against the formation of free radicals in the maintenance of the cell redox balance and in defense against electrophilic agents (Reischl et al, 2007). Moreover, metabolic parameters, like the amount of total amino acids, can reveal a stress response in which the organism is exposed.…”

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confidence: 99%

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Spatial and temporal biomarkers responses of Astyanax jacuhiensis (Cope, 1894)(Characiformes: Characidae) from the middle rio Uruguai, Brazil

et al. 2015

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Due to intense agricultural activity in the rio Uruguai (South Brazil), there is the potential for aquatic contamination by agrochemicals. In this region, there are many reservoirs to meet the water demand for rice fields, forming lentic environments. In line with this information, the aim of this study was to show a comparative analysis of some biomarkers, such as lipid peroxidation (TBARS), gluthatione S-transferase (GST), non-protein thiols (NPSH), amino acids (AA) and piscine micronucleus tests (MNE) in Astyanax jacuhiensis from lentic and lotic environments in the middle rio Uruguai region, comparing warm and cold seasons. Eight pesticides were found in water samples, with propoxur having the highest concentration found in both environments and seasons. Fish from the warm season showed higher levels of biochemical biomarkers, and fish from the cold season showed higher levels of MNE and AA. TBARS and AA presented higher levels in fish from the river, while GST, NPSH, MNE and AA presented higher levels in fish from dams. These environments have different characteristics in terms of redox potential, aeration, sedimentation, trophic structure, agrochemicals input and others, which may affect the physiological and biochemical responses of fish in against adverse situations.Devido à intensa atividade agrícola no rio Uruguai (Sul do Brasil), há potencial para contaminação aquática por agrotóxicos. Há muitos reservatórios para atender a demanda de água de campos de arroz, formando ambientes lênticos. De acordo com estas informações, o objetivo do presente estudo foi mostrar uma análise comparativa de alguns biomarcadores como a peroxidação lipídica (TBARS), glutationa S-transferase (GST), tióis não-protéicos (NPSH), aminoácidos (AA) e teste písceo de micronúcleos (MNE) em Astyanax jacuhiensis amostrados em ambientes lóticos e lênticos da região do médio rio Uruguai, comparando estações quentes e frias. Oito pesticidas foram encontrados em amostras de água, sendo propoxur a maior concentração encontrada em ambos os ambientes e estações. Peixes da estação quente apresentaram maiores níveis de biomarcadores bioquímicos e peixes da estação fria apresentaram maiores níveis de MNE e AA. TBARS e AA apresentaram maiores níveis nos peixes de rio, enquanto GST, NPSH, MNE e AA apresentaram níveis mais elevados em peixes da represa. Estes ambientes têm características diferentes, com potencial redox, aeração, sedimentação, estrutura trófica, a entrada de agroquímicos e outros que podem afetar as respostas fisiológicas e bioquímicas de peixe contra situação adversa.

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Section: Discussionsupporting

confidence: 70%

mentioning

confidence: 99%

Spatial and temporal biomarkers responses of Astyanax jacuhiensis (Cope, 1894)(Characiformes: Characidae) from the middle rio Uruguai, Brazil

et al. 2015

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“…Proteins achieve most of their antioxidant effect via its thiol groups (19,20). Additionally, the loss of the thiol group from proteins is the main molecular mechanism leading to structural and functional changes in proteins (18,19).…”

Section: Discussionmentioning

confidence: 99%

Thiol/Disulphide Homeostasis in Autosomal Dominant Polycystic Kidney Disease: A Single Center, Cross-Sectional Study

Esen¹,

Kahvecioğlu²,

Üstündağ³

et al. 2018

Turk Neph Dial Transpl

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OBJECTIVE:The aim of this study was to determine a novel oxidative stress marker, plasma thioldisulphide homoeostasis, in patients with autosomal dominant polycystic kidney disease (APKD). MATERIAL and METHODS:Thirty-four patients with autosomal dominant polycystic kidney disease and 30 healthy volunteers were included in the study. The thiol-disulfide homeostasis assay was performed. Total antioxidant capacity was evaluated via the FRAP assay. RESULTS:The median age was 46 (13) years in the patient group and 41 (13) years in the control group. In the APKD disease group, serum total and native thiol levels were statistically significantly lower (p = 0.002, p = 0.002, respectively). Serum native thiol levels were negatively correlated with age (r:-0.620 p: 0,000), systolic blood pressure (r: -0,697 p: 0,000), and diastolic blood pressure (r: -0,643 p: 0,000). A weak positive correlation was found between the disulfide/native thiol ratio and creatinine (r: 0.564 p <0.001), and a negative correlation was found between the disulfide/native thiol ratio and glomerular filtration rate (r: -0.372 p: 0.030). There was a significant positive correlation between the native thiol level and GFR (r: 0.699, p <0.001) level. CONCLUSION:Our findings suggest that thiol/disulfide homeostasis may be associated with autosomal dominant polycystic kidney disease progression. KEY WORDS:Oxidative stress, Autosomal dominant polycystic kidney disease, Thiol-disulphide, Creatinine ÖZ AMAÇ: Bu çalışmanın amacı, otozomal dominant polikistik böbrek (ODPK) hastalığı olanlarda yeni bir oksidatif stres markeri olan plazma tiyol-disülfid homeostazisini belirlemektir. GEREÇ ve YÖNTEMLER:Otozomal dominant polikistik böbrek hastalığı olan 34 hasta ve 30 sağlıklı gönüllü çalışmaya dahil edildi. Tiyol-disülfid homeostazı ölçüldü. Total antioksidan kapasite FRAP testi ile değerlendirildi. BULGULAR:Hastaların medyan yaşı 46 (13) yıl, kontrol grubunda 41 (13) yıldır. ODPK hastalarında serum total ve nativ tiyol düzeyleri istatistiksel olarak anlamlı derecede düşüktü (sırasıyla p=0,002, p=0,002). Serum nativ tiyol düzeyleri yaş (r:-0,620 p:0,000), sistolik kan basıncı (r:-0,697 p:0,000) ve diyastolik kan basıncıyla (r:-0,643 p:0,000) negatif korelasyon gösterdi. Disülfid / nativ tiyol oranı ile kreatinin arasında (r:0,564 p <0,001) zayıf pozitif korelasyon bulundu, disülfid / nativ tiyol oranı ve glomerüler filtrasyon hızı arasında negatif bir korelasyon bulundu (r:-0,372, p:0,030. Nativ tiyol düzeyi ile GFR arasında pozitif korelasyon bulundu (r:0,699, p<0,001). SONUÇ:Bulgularımız tiyol/disülfid homeostazının otozomal dominant polikistik böbrek hastalığının ilerlemesi ile ilişkili olabileceğini düşündürmektedir.

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“…Trachemys scipta was suggested to contain Hb with a high pool of reactive thiols with antioxidant capacity (Reischl et al, 2007), and we recently measured a total erythrocyte thiol concentration of 24 mmol l −1 in T. scripta (Jacobsen et al, 2012), which is much higher than in most vertebrates but similar to another freshwater turtle, Phrynops hilarii (Reischl, 1986). Given that the erythrocyte glutathione concentration is ~2 mmol l −1 (Reischl, 1986), it would seem that turtle Hb contains many thiols that might participate in quenching ROS produced inside the mitochondriacontaining erythrocytes when they re-oxygenate.…”

Section: Research Articlementioning

confidence: 99%

Nitric oxide metabolites during anoxia and reoxygenation in the anoxia-tolerant vertebrate, Trachemys scripta

Frank

Hansen

Montesanti

et al. 2013

Journal of Experimental Biology

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Moderate elevations of nitrite and nitric oxide (NO) protect mammalian tissues against ischemia (anoxia)-reperfusion damage by inhibiting mitochondrial electron transport complexes and reducing the formation of reactive oxygen species (ROS) upon reoxygenation. Crucian carp appear to exploit this mechanism by upregulating nitrite and other nitrite/NO metabolites (S-nitroso and iron-nitrosyl compounds) in several tissues when exposed to anoxia. We investigated whether this is a common strategy amongst anoxiatolerant vertebrates by evaluating NO metabolites in red-eared slider turtles during long-term (9 days) anoxia and subsequent reoxygenation at low temperature, a situation naturally encountered by turtles in ice-covered ponds. We also measured glutathione in selected tissues and assessed the impact of anoxia on electrolyte status. Anoxia induced major increases in [nitrite] in the heart, pectoral muscle and red blood cells, while [nitrite] was maintained unaltered in brain and liver. Concomitantly, the concentrations of Snitroso and iron-nitrosyl compounds increased, showing that nitrite was used to produce NO and to S-nitrosate cellular molecules during anoxia. The changes were gradually reversed during reoxygenation (1 h and 24 h), testifying that the processes were reversible. The increased NO bioavailability occurred in the absence of NO synthase activity (due to global anoxia) and may involve mobilization of internal/external nitrite reservoirs. Our data support the theory that anoxic upregulation of nitrite and other NO metabolites could be a general cytoprotective strategy amongst anoxia-tolerant vertebrates. The possible mechanisms of nitrite-derived NO and S-nitrosation in protecting cells from destructive Ca 2+ influx during anoxia and in limiting ROS formation during reoxygenation are discussed.

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Distribution, adaptation and physiological meaning of thiols from vertebrate hemoglobins

Cited by 48 publications

References 147 publications

Spatial and temporal biomarkers responses of Astyanax jacuhiensis (Cope, 1894)(Characiformes: Characidae) from the middle rio Uruguai, Brazil

Spatial and temporal biomarkers responses of Astyanax jacuhiensis (Cope, 1894)(Characiformes: Characidae) from the middle rio Uruguai, Brazil

Thiol/Disulphide Homeostasis in Autosomal Dominant Polycystic Kidney Disease: A Single Center, Cross-Sectional Study

Nitric oxide metabolites during anoxia and reoxygenation in the anoxia-tolerant vertebrate, Trachemys scripta

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