Distress Analysis of Mice with Cervical Arteriovenous Fistulas

Xie, Wenjie; Palme, Rupert; Schafmayer, Clemens; Zechner, Dietmar; Vollmar, Brigitte; Grambow, Eberhard

doi:10.3390/ani11113051

Cited by 1 publication

(1 citation statement)

References 31 publications

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“…In general, the assessment of animal wellbeing is often based on physiological parameters such as body weight [ 4 , 5 , 6 ] or fecal corticosterone metabolites (FCMs) [ 7 , 8 , 9 , 10 , 11 ]. In addition, clinical signs of distress [ 12 , 13 ]) or behavior such as nesting [ 14 , 15 , 16 , 17 , 18 ] burrowing [ 18 , 19 , 20 , 21 ] or wheel-running [ 22 , 23 ] are also often assessed as indicators for wellbeing of rodents. Although it is believed that a combination of different methods yields a more precise conclusion than relying on single parameters [ 24 , 25 , 26 ], these single parameters should ideally be sensitive enough on their own to discriminate between distressed and non-distressed animals.…”

Section: Introductionmentioning

confidence: 99%

Generalizability, Robustness and Replicability When Evaluating Wellbeing of Laboratory Mice with Various Methods

Zechner

Schulz

Tang

et al. 2022

Animals

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An essential basis for objectively improving the status of animals during in vivo research is the ability to measure the wellbeing of animals in a reliable and scientific manner. Several non-invasive methods such as assessing body weight, burrowing activity, nesting behavior, a distress score and fecal corticosterone metabolites were evaluated in healthy mice and after three surgical interventions or during the progression of four gastrointestinal diseases. The performance of each method in differentiating between healthy and diseased animals was assessed using receiver operating characteristic curves. The ability to differentiate between these two states differed between distinct surgical interventions and distinct gastrointestinal diseases. Thus, the generalizability of these methods for assessing animal wellbeing was low. However, the robustness of these methods when assessing wellbeing in one gastrointestinal disease was high since the same methods were often capable of differentiating between healthy and diseased animals independent of applied drugs. Moreover, the replicability when assessing two distinct cohorts with an identical surgical intervention was also high. These data suggest that scientists can reach valid conclusions about animal wellbeing when using these methods within one specific animal model. This might be important when optimizing methodological aspects for improving animal wellbeing. The lack of generalizability, however, suggests that comparing animal models by using single methods might lead to incorrect conclusions. Thus, these data support the concept of using a combination of several methods when assessing animal welfare.

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