Distinguishing Zika and Dengue Viruses through Simple Clinical Assessment, Singapore

Yan, Gabriel; Pang, Long; Cook, Alex R.; Ho, Hanley J.; Win, Mar Soe; Khoo, Ai Leng; Wong, Joshua; Lee, Chun Kiat; Yan, Benedict; Jureen, Roland; Ho, Siew Seen; Lye, David C.; Tambyah, Paul Anantharajah; Leo, Yee Sin; Fisher, Dale; Oon, Jolene; Bagdasarian, Natasha; Chow, Angela; Smitasin, Nares; Chai, Louis Yi Ann

doi:10.3201/eid2408.171883

Cited by 33 publications

(42 citation statements)

References 9 publications

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“…Dengue and coronavirus disease 2019 (COVID-19) are difficult to distinguish because they have shared clinical and laboratory features. 1, 2 We describe two patients in Singapore with falsepositive results from rapid serological testing for dengue, who were later confirmed to have severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, the causative virus of COVID-19.…”

Section: Covert Covid-19 and False-positive Dengue Serology In Singaporementioning

confidence: 99%

Covert COVID-19 and false-positive dengue serology in Singapore

Yan

Lee

Lam

et al. 2020

The Lancet Infectious Diseases

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330

332

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Section: Covert Covid-19 and False-positive Dengue Serology In Singaporementioning

confidence: 99%

Covert COVID-19 and false-positive dengue serology in Singapore

Yan

Lee

Lam

et al. 2020

The Lancet Infectious Diseases

Self Cite

330

332

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“…In the dengue virus, 14-3-3 ε binds to the NS3 protein and prevents RIG-I from translocating to the mitochondrial antiviral signaling (MAVS) protein adapter, thereby blocking antiviral signaling [76]. Liu et al reported 14-3-3 ε as a critical mediator for the relocation of RIG-I from cytosol to mitochondrial-associated MAVS, as it establishes a translocon complex with RIG-I and TRIM25, eventually triggering an antiviral response [78].…”

Section: Role Of 14-3-3 Family In Rna Virusesmentioning

confidence: 99%

“…Additionally, our group has shown that N protein induces apoptosis by reducing the expression level of 14-3-3 θ in the absence of growth factors (serum), which contributes to the accumulation of the phosphorylated N protein in the nucleus [78]. Likewise, coronavirus 2, or COVID-19, originated from China sharing similar demographic profiles, and laboratory and radiological findings with SARS and Middle East respiratory syndrome (MERS) [80].…”

Section: Role Of 14-3-3 Family In Rna Virusesmentioning

confidence: 99%

“…Interactions between 14-3-3 and BPLF1 may participate in the regulation of many signaling pathways; ubiquitin ligases, cullin 1 (CUL1) and tripartite motif-containing protein 25 (TRIM25) are the possible partners in this regulation. Furthermore, 14-3-3 proteins and TRIM25 serve as vital co-factors in the signaling of viral nucleic acid sensors, such as retinoic acid-inducible gene I (RIG-I) and MDA5 [78]. In this case, 14-3-3 proteins stabilize the interaction of TRIM25 with RIG-I, thus facilitating the ubiquitination of RIG-I [89].…”

Section: Role Of 14-3-3 In Dna Virusesmentioning

confidence: 99%

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The Multifarious Role of 14-3-3 Family of Proteins in Viral Replication

Nathan

Lal

2020

Viruses

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The 14-3-3 proteins are a family of ubiquitous and exclusively eukaryotic proteins with an astoundingly significant number of binding partners. Their binding alters the activity, stability, localization, and phosphorylation state of a target protein. The association of 14-3-3 proteins with the regulation of a wide range of general and specific signaling pathways suggests their crucial role in health and disease. Recent studies have linked 14-3-3 to several RNA and DNA viruses that may contribute to the pathogenesis and progression of infections. Therefore, comprehensive knowledge of host–virus interactions is vital for understanding the viral life cycle and developing effective therapeutic strategies. Moreover, pharmaceutical research is already moving towards targeting host proteins in the control of virus pathogenesis. As such, targeting the right host protein to interrupt host–virus interactions could be an effective therapeutic strategy. In this review, we generated a 14-3-3 protein interactions roadmap in viruses, using the freely available Virusmentha network, an online virus–virus or virus–host interaction tool. Furthermore, we summarize the role of the 14-3-3 family in RNA and DNA viruses. The participation of 14-3-3 in viral infections underlines its significance as a key regulator for the expression of host and viral proteins.

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“…Consistently, it has been found down-regulated in platelets from dengue patients compared to control 18 , as high levels can promote rapid replication and propagation of the virus 19 . Indeed, the platelet count is one of the diagnostic indices used to distinguish between DENV and ZIKV infections 20 . GSN (Gelsolin) binds to the plus ends of actin monomers or filaments.…”

Section: Machine Learning Highlights Potential New Diagnostic Markersmentioning

confidence: 99%

High-resolution proteomics identifies potential new markers of Zika and dengue infections

Allgoewer

Zhao

Maity

et al. 2019

Preprint

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Distinguishing between Zika and dengue virus infections is critical for treatment and anticipation of complications. However, existing biomarkers have high error rates. To identify new potential diagnostic signatures, we used next-generation proteomics to profile 122 serum samples from 62 Zika or dengue patients. We quantified >500 proteins and identified 26 proteins that were significantly differentially expressed. These proteins typically function in infection and wound healing, with several also linked to pregnancy and brain. Integrating machine learning approaches, we used 7 proteins to predict ZIKV infection correctly in 72% of the cases, outperforming other tools. The three most predictive proteins were Platelet Factor 4 Variant 1, Fibrinogen Alpha, and Gelsolin. Finally, we showed that temporal changes in protein signatures from the same patient can disambiguate some diagnoses and serve as indicators for past infections. Taken together, we demonstrate that serum proteomics can be highly valuable to diagnose even challenging samples.

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Distinguishing Zika and Dengue Viruses through Simple Clinical Assessment, Singapore

Cited by 33 publications

References 9 publications

Covert COVID-19 and false-positive dengue serology in Singapore

Covert COVID-19 and false-positive dengue serology in Singapore

The Multifarious Role of 14-3-3 Family of Proteins in Viral Replication

High-resolution proteomics identifies potential new markers of Zika and dengue infections

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