Distinguishing the viability of a single yeast cell with an ultra-sensitive radio frequency sensor

Abstract

We propose and demonstrate a simple, ultra sensitive radio frequency (RF) sensor to detect a single yeast cell and distinguish its viability in a microfluidic channel. On-chip interference is used to cancel background probing signals to improve sensor sensitivity. Individual viable and nonviable yeast cells (approximately 5.83 +/- 0.85 microm in diameter) are measured with clear sensing and identification of these cells.

Measuring the microwave permittivity of single particles

“…Recent efforts on microwave characterization of single cells include resonator based circuits [5,6] and our previous work in [7,8]. Here we report our results in developing new microwave sensors, measuring single particles in liquids, and extracting the microwave permittivity of these particles with a new algorithm proposed in this work.…”

Three-dimensional electrokinetic particle focusing in a rectangular microchannel

“…Due to the wall-induced electrical repulsion force, neutrally buoyant particles in electrophoresis were observed to migrate toward and travel along the channel axis. Non-neutral particles are, however, anticipated to flow in a focused stream near the top or bottom wall, onto which sensing electrodes may be fabricated for improved electrical detections [7]. Such autonomous particle focusing in a rectangular microchannel may potentially be used in microflow cytometers.…”

A Radio Frequency Sensor For Measurement Of Small Dielectric Property Changes

“…Sensors based on measuring dielectric property changes [6][7][8], such as surface moisture sensors, have been demonstrated as well. Dielectric spectroscopy that measures dielectric properties and their changes has several advantages, including large volume manufacturing and easy integration with control circuitry [9].…”