Distinguishing the dominant species of pathogen in ethmoidal sinusitis by sequencing DNA dataset analysis

Abstract

Identifying the predominant microbial species in patients with ethmoidal sinusitis is conducive to its successful treatment. The aim of the present study was to determine the microbial composition and the predominant fungal and bacterial species in patients with ethmoidal sinusitis. A sample was obtained from 3 patients with ethmoidal sinusitis and from the ethmoid sinus of 2 healthy volunteers. Those samples were sequenced using an Illumina/Solexa sequencing platform for mapping to human, fungal, and bacterial genomes. Fungal and bacterial expressions in those samples were analyzed through bioinformatics and statistical methods. The sequencing data revealed that the dominant fungal strains in the ethmoidal sinusitis samples compared with the healthy controls (8_S33 and 10_S9) were Aspergillus oryzae and Aspergillus flavus, and the dominant bacterial strains were Haemophilus influenzae and Haemophilus parainfluenzae. Together, these findings indicate that the development of ethmoidal sinusitis is associated with the presence of fungi and bacteria, which may benefit the successful diagnosis and treatment for patients with ethmoidal sinusitis.

Analysis of Microbiota and Mycobiota in Fungal Ball Rhinosinusitis: Specific Interaction between Aspergillus fumigatus and Haemophilus influenza?

“…However, Haemophilus are bacteria that are sometimes difficult to grow, requiring specific vitamins-enriched media [ 62 ]. Working on the sequencing of three ethmoidal sinus samples, the same authors mainly found reads from Aspergillus and Haemophilus [ 65 ]. Interestingly, a study investigating allergic fungal RS ( n = 4) and chronic RS ( n = 7) observed polymicrobial biofilms with Haemophilus and fungal elements in 82% of sinus biopsies [ 17 ].…”

Microbiota Dysbiosis in Fungal Rhinosinusitis

“…Previous studies have discovered inter-microbial interactions between Aspergillus and bacteria [11,12,13,14,15,16]. Haemophilus influenzae and Haemophilus parainfluenzae were reported in ethmoidal sinusitis samples that were analyzed through DNA sequencing using an Illumina/Solexa sequencing platform, and Aspergillus oryzae and Aspergillus flavus were the dominant fungal species [11]. H. influenza was also observed to be a possible coinfection with A. flavus in dual infectious brainstem encephalitis, which may be related to chronic fungal sinusitis [12].…”