Distinguishing Methicillin-Resistant and Sensitive <i>Staphylococcus aureus</i> Using Volatile Headspace Metabolites

Jia, Beike; Sohnlein, B H; Mortelmans, Kristien; Coggiola, M. J.; Oser, Harald

doi:10.1109/jsen.2009.2035671

Cited by 13 publications

(12 citation statements)

References 9 publications

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“…We were unable to specify this metabolite as a product of anabolism or catabolism, but one possibility is that the propionic acid portions of porphobilinogen are stripped off as easily available carbon and energy sources by S. epidermidis , and the 2,4-dimethyl-1H-pyrrole is discarded as a catabolic byproduct. Additional compounds detected solely in the filtrates of S. epidermidis were benzaldehyde (MHB only), 2-phenylacetaldehyde (all media), and (methyldisulfanyl)methane (BHI and LB), though all three compounds have been reported in prior analyses of S. aureus volatilomes [ 38 , 39 , 40 , 41 ].…”

Section: Resultsmentioning

confidence: 96%

Dependence of the Staphylococcal Volatilome Composition on Microbial Nutrition

Jenkins

Bean

2020

Metabolites

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In vitro cultivation of staphylococci is fundamental to both clinical and research microbiology, but few studies, to-date, have investigated how the differences in rich media can influence the volatilome of cultivated bacteria. The objective of this study was to determine the influence of rich media composition on the chemical characteristics of the volatilomes of Staphylococcus aureus and Staphylococcus epidermidis. S. aureus (ATCC 12600) and S. epidermidis (ATCC 12228) were cultured in triplicate in four rich complex media (brain heart infusion (BHI), lysogeny broth (LB), Mueller Hinton broth (MHB), and tryptic soy broth (TSB)), and the volatile metabolites produced by each culture were analyzed using headspace solid-phase microextraction combined with comprehensive two-dimensional gas chromatography—time-of-flight mass spectrometry (HS-SPME-GC×GC-TOFMS). When comparing the chemical compositions of the staph volatilomes by the presence versus absence of volatiles produced in each medium, we observed few differences. However, when the relative abundances of volatiles were included in the analyses, we observed that culturing staph in media containing free glucose (BHI and TSB) resulted in volatilomes dominated by acids and esters (67%). The low-glucose media (LB and MHB) produced ketones in greatest relative abundances, but the volatilome compositions in these two media were highly dissimilar. We conclude that the staphylococcal volatilome is strongly influenced by the nutritional composition of the growth medium, especially the availability of free glucose, which is much more evident when the relative abundances of the volatiles are analyzed, compared to the presence versus absence.

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Section: Resultsmentioning

confidence: 96%

Dependence of the Staphylococcal Volatilome Composition on Microbial Nutrition

Jenkins

Bean

2020

Metabolites

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“…The ability of volatile metabolites to differentiate between CPE and non-CPE has never previously been assessed, although prior studies have demonstrated the utility of this approach in discriminating between methicillin-resistant and methicillin-susceptible Staphylococcus aureus (MRSA and MSSA, respectively) 36 – 39 , and between vancomycin-resistant and vancomycin-susceptible Enterococcus (VRE and VSE, respectively) 38 . Like CP K .…”

Section: Discussionmentioning

confidence: 99%

Detection of high-risk carbapenem-resistant Klebsiella pneumoniae and Enterobacter cloacae isolates using volatile molecular profiles

Rees

Nasir

Smolinska

et al. 2018

Sci Rep

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Infections caused by carbapenem-resistant Enterobacteriaceae (CRE) are alarming in the clinical setting, as CRE isolates often exhibit resistance to most clinically-available antibiotics. Klebsiella pneumoniae carbapenemase (KPC) is the most common carbapenemase carried by CRE in North America and Europe, frequently detected in isolates of K. pneumoniae, Escherichia coli, and Enterobacter cloacae. Notably, KPC-expressing strains often arise from clonal lineages, with sequence type 258 (ST258) representing the dominant lineage in K. pneumoniae, ST131 in E. coli, and ST78 and ST171 in E. cloacae. Prior studies have demonstrated that carbapenem-resistant K. pneumoniae differs from carbapenem-susceptible K. pneumoniae at both the transcriptomic and soluble metabolomic levels. In the present study, we sought to determine whether carbapenem-resistant and carbapenem-susceptible isolates of K. pneumoniae, E. coli, and E. cloacae produce distinct volatile metabolic profiles. We were able to identify a volatile metabolic fingerprint that could discriminate between CRE and non-CRE with an area under the receiver operating characteristic curve (AUROC) as high as 0.912. Species-specific AUROCs were as high as 0.988 for K. pneumoniae and 1.000 for E. cloacae. Paradoxically, curing of KPC-expressing plasmids from a subset of K. pneumoniae isolates further accentuated the metabolic differences observed between ST258 and non-ST258.

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“…Bacteria must be cultivated in specific culture media [ 18 , 19 , 20 ] containing 10% glucose and sulfur compounds, as the triple-sugar medium iron (TSI) allows the synthesis of a greater diversity of VOCsB [ 21 ]. In the extraction of VOCsB, static headspace solid-phase microextraction (SHS-SPME) has been used to capture these compounds; due to the sensitivity and how they adapt to the bacterial culture, the fibers that have been used in various studies are those of polydimethylsiloxane (PDMS), polyacrylate (PA), and/or divinylbenzene (DVB) for VOCsB of Enterobacteriaceae [ 22 , 23 , 24 ]. However, SHS-SPME is a destructive test, which limits its use in bioassays, so dynamic headspace Super Q solid-phase extraction (DHS-SPE) [ 25 , 26 ] is an alternative to obtain samples that can be tested in attraction bioassays; the two forms of extraction are followed by VOCsB identification by gas chromatographic mass-spectrometry (GC-MS) [ 19 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Attraction of Adults of Cyclocephala lunulata and Cyclocephala barrerai (Coleoptera: Scarabaeoidea: Melolonthidae) towards Bacteria Volatiles Isolated from Their Genital Chambers

et al. 2020

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In the study of the chemical communication of adults of the Melolonthidae family, bacteria have been observed in the epithelium of the genital chamber; possibly, bacteria are involved in the production of sex attractants in their hosts. Therefore, it is important to identify the volatile organic compounds from bacteria (VOCsB) released by these microorganisms and study the biological activity stimulated by VOBCs in adults of Melolonthidae. In this study, bacteria were isolated from the genital chamber of Cyclocephala lunulata and Cyclocephala barrerai, from which VOCsB were extracted using static headspace solid-phase microextraction (SHS-SPME) and dynamic headspace Super Q solid-phase extraction (DHS-SPE) and analyzed using gas chromatography-mass spectrometry. The effect of VOCsB on the hosts and conspecifics was evaluated utilizing an olfactometer and electroantennography (EAG). Two species of Enterobacteria were isolated from the genital chamber of each female species, and VOCsB derived from sulfur-containing compounds, alcohols, esters, and fatty acids were identified. An attraction response was observed in olfactometry studies, and antennal responses to VOCsB were confirmed in EAG bioassays. With these results, new perspectives on the relationship between these beetles and their bacteria emerge, in addition to establishing a basis for management programs in the future.

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Distinguishing Methicillin-Resistant and Sensitive Staphylococcus aureus Using Volatile Headspace Metabolites

Cited by 13 publications

References 9 publications

Dependence of the Staphylococcal Volatilome Composition on Microbial Nutrition

Dependence of the Staphylococcal Volatilome Composition on Microbial Nutrition

Detection of high-risk carbapenem-resistant Klebsiella pneumoniae and Enterobacter cloacae isolates using volatile molecular profiles

Attraction of Adults of Cyclocephala lunulata and Cyclocephala barrerai (Coleoptera: Scarabaeoidea: Melolonthidae) towards Bacteria Volatiles Isolated from Their Genital Chambers

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